In vitro growth characteristics of rat mesothelioma cells in culture.

Abstract:

:The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for normal RPMC. Neoplastic RPMC cultures had a population doubling time of 19 hr versus 30 hr for the normal cells. Plating efficiency in liquid medium was almost 100%. Cultures of neoplastic RPMC were anchorage-independent since 70% of the seeded cells formed colonies after one week; after the second week, colony size was enhanced but colony recovery was not. The serum dependence of neoplastic cells was less than that of the normal cells. 79 out of 100 metaphase cells analyzed had 41 to 43 chromosomes, and the modal number was 42 (38%). A large metacentric chromosome was observed in 77 of the 100 neoplastic metaphase cells analyzed, but not in any normal metaphase cells. In nude mice, the neoplastic RPMC were tumorigenic.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Jaurand MC,Renier A,Van der Meeren A,Magne L,Pinchon MC,Bignon J

doi

10.1111/j.1768-322x.1986.tb00481.x

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

249-56

issue

3

eissn

0248-4900

issn

1768-322X

journal_volume

57

pub_type

杂志文章
  • The cytoskeleton of mammalian spermatozoa.

    abstract::The mammalian spermatozoa are endowed with a unique cytoskeleton which consists both of ubiquitous and specific proteins, some of them arising from gene haploid transcription. In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants. In the ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(94)80001-4

    authors: Fouquet JP,Kann ML

    更新日期:1994-01-01 00:00:00

  • Fission yeast cytoskeletons and cell polarity factors: connecting at the cortex.

    abstract::Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20060048

    authors: La Carbona S,Le Goff C,Le Goff X

    更新日期:2006-11-01 00:00:00

  • ERM proteins: from cellular architecture to cell signaling.

    abstract::ERM (ezrin/radixin/moesin) proteins, concentrated in actin rich cell-surface structures, cross-link actin filaments with the plasma membrane. They are involved in the formation of microvilli, cell-cell adhesion, maintenance of cell shape, cell motility and membrane trafficking. Recent analyses reveal that they are not...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(00)01078-9

    authors: Louvet-Vallée S

    更新日期:2000-08-01 00:00:00

  • Control of CREB phosphorylation and its role for induction of melatonin synthesis in rat pinealocytes.

    abstract::Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(98)80006-3

    authors: Maronde E,Schomerus C,Stehle JH,Korf HW

    更新日期:1997-11-01 00:00:00

  • Immunocytochemical localization of arabinoxylans in the cell wall of maize apical internode after microbial degradation in the rumen.

    abstract::Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1996.tb00988.x

    authors: Migné C,Prensier G,Cornu A,Grenet E

    更新日期:1996-01-01 00:00:00

  • A hypothesis on p34cdc2 sequestration based on the existence of Ca(2+)-coordinated changes in H+ and MPF activities during Xenopus egg activation [corrected].

    abstract::The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/0248-4900(92)90137-p

    authors: Charbonneau M,Grandin N

    更新日期:1992-01-01 00:00:00

  • F3/contactin, a neuronal cell adhesion molecule implicated in axogenesis and myelination.

    abstract::A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(02)00006-0

    authors: Falk J,Bonnon C,Girault JA,Faivre-Sarrailh C

    更新日期:2002-10-01 00:00:00

  • Length of the selectivity filter of aquaporin-1.

    abstract::We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Whittembury G,González E,Gutiérrez AM,Echevarría M,Hernández CS

    更新日期:1997-08-01 00:00:00

  • Bistramide A-induced irreversible arrest of cell proliferation in a non-small-cell bronchopulmonary carcinoma is similar to induction of terminal maturation.

    abstract::Bistramide A, a new toxin isolated from a New Caledonian Urochordata, shows an antiproliferative effect on a non-small-cell lung carcinoma line in vitro and G1-blockade. In this work, the growth arrest induced by bistramide A was shown to be irreversible as assessed by growth kinetics of pretreated cells. Furthermore,...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(05)80196-0

    authors: Riou D,Roussakis C,Robillard N,Biard JF,Verbist JF

    更新日期:1993-01-01 00:00:00

  • Once upon a time there was beta-catenin in cadherin-mediated signalling.

    abstract::beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040531

    authors: Gavard J,Mège RM

    更新日期:2005-12-01 00:00:00

  • Triiodothyronine influences quail myoblast proliferation and differentiation.

    abstract::The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(93)90129-3

    authors: Marchal S,Cassar-Malek I,Pons F,Wrutniak C,Cabello G

    更新日期:1993-01-01 00:00:00

  • Induction and specification of the vertebrate ectodermal placodes: precursors of the cranial sensory organs.

    abstract::The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20040515

    authors: Brugmann SA,Moody SA

    更新日期:2005-05-01 00:00:00

  • Regulation of primary spinal neuron lineages after deletion of a major progenitor.

    abstract::Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/j.biolcel.2004.06.002

    authors: Gallagher BC,Moody SA

    更新日期:2004-09-01 00:00:00

  • Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry.

    abstract::Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction)...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00402.x

    authors: Barni S,Gerzeli G

    更新日期:1985-01-01 00:00:00

  • Pathology of the cytoskeleton of the human sperm flagellum: axonemal and peri-axonemal anomalies.

    abstract::A quantitative ultrastructural study was performed on 56 ejaculates showing anomalies of the sperm axonemal complex. The anomalies comprised either the absence of one, or more often several, axonemal structures, or defective elongation of the doublets. Several characteristics relating to the extent and superimposition...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1984.tb00253.x

    authors: Escalier D,David G

    更新日期:1984-01-01 00:00:00

  • Higher eucaryotic cdc25 proteins are structurally related to phosphoseryl/threonyl protein phosphatases.

    abstract::cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90134-m

    authors: Bellé R,Ollivier E,Guerrucci MA

    更新日期:1992-01-01 00:00:00

  • Inflammatory environment induces gingival tissue-specific mesenchymal stem cells to differentiate towards a pro-fibrotic phenotype.

    abstract:BACKGROUND INFORMATION:Human gingival tissues are prone to hyperplasia under inflammatory stimuli. We have identified gingival tissue-specific mesenchymal stem cells (GMSCs) and found their functional change being correlated with drug-induced gingival hyperplasia. However, whether these cells exhibit characteristics of...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201200064

    authors: Li N,Liu N,Zhou J,Tang L,Ding B,Duan Y,Jin Y

    更新日期:2013-06-01 00:00:00

  • Presence of Chromatium vinosum chaperonins 10 and 60 in mitochondria and peroxisomes of rat hepatocytes.

    abstract::In the present study we report the occurrence of chaperonins, cpn10 and cpn60, in Chromatium vinosum and rat hepatocytes, using specific polyclonal antibodies in conjunction with the protein A-gold immunocytochemical technique. As demonstrated by quantitative evaluations, the immunolabeling for cpn10 and cpn60 in C vi...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1995.tb00943.x

    authors: Vélez-Granell CS,Arias AE,Torres-Ruíz JA,Bendayan M

    更新日期:1995-01-01 00:00:00

  • Poly(A)-binding protein and eRF3 are associated in vivo in human and Xenopus cells.

    abstract::An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(02)01194-2

    authors: Cosson B,Berkova N,Couturier A,Chabelskaya S,Philippe M,Zhouravleva G

    更新日期:2002-09-01 00:00:00

  • Distribution of sugar-binding sites within interphase nuclei and mitotic chromosomes of a human cell line.

    abstract::Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1990.tb00007.x

    authors: Bourgeois CA,Monsigny M

    更新日期:1990-01-01 00:00:00

  • Cytochalasin D induces changes in cell shape and promotes in vitro chondrogenesis: a morphological study.

    abstract::One of the initial events required for the expression of cartilage-specific macromolecules in monolayer cultures is the reversion to the initial round shape of chondrocytes. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of cultured chondrocytes. Our s...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(96)81303-7

    authors: Loty S,Forest N,Boulekbache H,Sautier JM

    更新日期:1995-01-01 00:00:00

  • Densitometric analysis of nuclear DNA content in lentil roots grown in space.

    abstract::Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(94)90055-8

    authors: Driss-Ecole D,Schoevaert D,Noin M,Perbal G

    更新日期:1994-01-01 00:00:00

  • Aquaporin-1 in the peritoneal membrane: implications for peritoneal dialysis and endothelial cell function.

    abstract::PD (peritoneal dialysis) is an established mode of renal replacement therapy, based on the exchange of fluid and solutes between blood in peritoneal capillaries and a dialysate that has been introduced into the peritoneal cavity. The dialysis process involves diffusive and convective transports and osmosis through the...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20040132

    authors: Devuyst O,Ni J,Verbavatz JM

    更新日期:2005-09-01 00:00:00

  • Ajuba: a new microtubule-associated protein that interacts with BUBR1 and Aurora B at kinetochores in metaphase.

    abstract:BACKGROUND INFORMATION:The role of the LIM-domain-containing protein Ajuba was initially described in cell adhesion and migration processes and recently in mitosis as an activator of the Aurora A kinase. RESULTS:In the present study, we show that Ajuba localizes to centrosomes and kinetochores during mitosis. This loc...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20080060

    authors: Ferrand A,Chevrier V,Chauvin JP,Birnbaum D

    更新日期:2009-04-01 00:00:00

  • Structural and immunohistological modifications in olfactory bulb of the staggerer mutant mouse.

    abstract::In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Monnier Z,Bahjaoui-Bouhaddi M,Bride J,Bride M,Math F,Propper A

    更新日期:1999-01-01 00:00:00

  • Sec61 blockade by mycolactone: A central mechanism in Buruli ulcer disease.

    abstract::Infection with Mycobacterium ulcerans results in a necrotising skin disease known as a Buruli ulcer, the pathology of which is directly linked to the bacterial production of the toxin mycolactone. Recent studies have identified the protein translocation machinery of the endoplasmic reticulum (ER) membrane as the prima...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/boc.201800030

    authors: Demangel C,High S

    更新日期:2018-11-01 00:00:00

  • Ultrastructural in situ hybridization: a review of technical aspects.

    abstract::Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:

    authors: Le Guellec D

    更新日期:1998-07-01 00:00:00

  • Construction and characterization of a DNA library from mouse chromosomes 19 purified by flow cytometry.

    abstract::In spite of the constant development concerning physical mapping of eukaryotic genomes, the mouse chromosome 19 remains poorly characterized. In order to improve the possibilities for studying this chromosome, we have constructed a chromosome-specific EcoRI DNA fragment library from mouse chromosomes 19 sorted by flow...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90322-t

    authors: Baron B,Métézeau P,Kiefer-Gachelin H,Goldberg ME

    更新日期:1990-01-01 00:00:00

  • Histochemical and biochemical studies of cholinesterases in the carotid labyrinth of amphibians.

    abstract::Acetylcholinesterase activity was detected in the carotid labyrinth of amphibians by both biochemical and histochemical methods. The histochemical tests showed enzyme activity in surfaces of afferent and efferent nerve terminals in contact with type I cells, but none within the type I cells. Acetylcholinesterase activ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00374.x

    authors: Poullet M,Verdiere M

    更新日期:1985-01-01 00:00:00

  • The periseptal annulus in Escherichia coli.

    abstract::Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1984.tb00274.x

    authors: Anba J,Bernadac A,Pages JM,Lazdunski C

    更新日期:1984-01-01 00:00:00