Abstract:
:The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for normal RPMC. Neoplastic RPMC cultures had a population doubling time of 19 hr versus 30 hr for the normal cells. Plating efficiency in liquid medium was almost 100%. Cultures of neoplastic RPMC were anchorage-independent since 70% of the seeded cells formed colonies after one week; after the second week, colony size was enhanced but colony recovery was not. The serum dependence of neoplastic cells was less than that of the normal cells. 79 out of 100 metaphase cells analyzed had 41 to 43 chromosomes, and the modal number was 42 (38%). A large metacentric chromosome was observed in 77 of the 100 neoplastic metaphase cells analyzed, but not in any normal metaphase cells. In nude mice, the neoplastic RPMC were tumorigenic.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Jaurand MC,Renier A,Van der Meeren A,Magne L,Pinchon MC,Bignon Jdoi
10.1111/j.1768-322x.1986.tb00481.xsubject
Has Abstractpub_date
1986-01-01 00:00:00pages
249-56issue
3eissn
0248-4900issn
1768-322Xjournal_volume
57pub_type
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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