Abstract：
:Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change primary spinal neuronal fates after deletion of a major spinal cord progenitor. After ablation of the V1.2 blastomere of the 16-cell Xenopus embryo, the spinal cord contained normal numbers of Rohon-Beard neurons and primary motoneurons, indicating that the remaining blastomeres numerically reconstituted these populations. Using lineage-tracing techniques we revealed a global response: 10 out of the 15 remaining blastomeres significantly changed the number of one or both neuronal types they produced. This widespread response indicates that position in the early embryo plays an important role in regulating the production of primary spinal neurons. However, not all cells are influenced solely by position; a vegetal cell transplanted into the position of the deleted V1.2 did not take on the neuronal fate of its new position. Thus, restitution of pattern relies on a combination of positional cues and intrinsic fate restrictions.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Gallagher BC,Moody SAdoi
10.1016/j.biolcel.2004.06.002subject
Has Abstractpub_date
2004-09-01 00:00:00pages
539-44issue
7eissn
0248-4900issn
1768-322Xpii
S0248-4900(04)00126-1journal_volume
96pub_type
杂志文章abstract：:Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00375.x
更新日期：1985-01-01 00:00:00
abstract：:The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00361.x
更新日期：1985-01-01 00:00:00
abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90340-9
更新日期：1990-01-01 00:00:00
abstract：:Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(98)80006-3
更新日期：1997-11-01 00:00:00
abstract：BACKGROUND INFORMATION:There have been several studies to understand the influence of stiffness of the culture substrates for different types of adherent cells. It is generally accepted that cell proliferation, spreading and focal adhesions increase with higher matrix stiffness. However, what remains unclear is whether...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201900115
更新日期：2020-10-01 00:00:00
abstract：:The interaction of cells with extracellular matrix components contributes to their specific differentiation. We studied hepatic peroxisomes and their changing features during embryonic development, and we immunolocalized in the same tissue the extracellular matrix components laminin and collagen IV as well as the inte...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:Structured mRNA elements called riboswitches control gene expression by binding to small metabolites. Over a dozen riboswitch classes have been characterized that target a broad range of molecules and vary widely in size and secondary structure. Four of the known riboswitch classes recognize purines or modified purine...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20070088
更新日期：2008-01-01 00:00:00
abstract：:ERM (ezrin/radixin/moesin) proteins, concentrated in actin rich cell-surface structures, cross-link actin filaments with the plasma membrane. They are involved in the formation of microvilli, cell-cell adhesion, maintenance of cell shape, cell motility and membrane trafficking. Recent analyses reveal that they are not...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01078-9
更新日期：2000-08-01 00:00:00
abstract：BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300003
更新日期：2013-08-01 00:00:00
abstract：:The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90343-2
更新日期：1990-01-01 00:00:00
abstract：:This study was undertaken to evaluate the effects of caerulein, a CCK analog, on the different cell populations of the pancreatic tissue and their respective turnover. Rats received saline or caerulein subcutaneously and 3H-thymidine intraperitoneally three times a day for 4 days. They were sacrificed immediately afte...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The mammalian spermatozoa are endowed with a unique cytoskeleton which consists both of ubiquitous and specific proteins, some of them arising from gene haploid transcription. In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants. In the ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(94)80001-4
更新日期：1994-01-01 00:00:00
abstract：:The communication of calcium signals between cells is known to be operative between neurons where these signals integrate intimately with electrical and chemical signal communication at synapses. Recently, it has become clear that glial cells also exchange calcium signals between each other in cultures and in brain sl...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/j.biolcel.2003.10.007
更新日期：2004-02-01 00:00:00
abstract：:After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various siz...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201600042
更新日期：2017-02-01 00:00:00
abstract：:Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1996.tb00988.x
更新日期：1996-01-01 00:00:00
abstract：:During a historical research, we realized that René Couteaux (1909-1999) was the first histologist who stained the postsynaptic structure of the neuromuscular junction. By means of Janus Green B dye, he revealed the membranous 'subneural apparatus' related to the 'synaptic gutter'. Hence, he justified the use of the p...
journal_title：Biology of the cell
pub_type： 传,历史文章,杂志文章
doi：10.1042/BC20050036
更新日期：2006-08-01 00:00:00
abstract：:The internalization of the yolk proteins has been investigated by electron microscopy and cytochemistry in the oocyte of the trout which stores up large quantities of yolk. The oocyte evolution has been followed for 18 months in a homogeneous group of animals. Anionic ferritin has been injected during vitellogenesis. ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00283.x
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Caspases are cysteine proteases that mediate apoptosis (programmed cell death) initiation and execution. Apoptosis is a conserved mechanism shared by all metazoans, although its physiological function and complexity show considerable taxon-dependent variations. To gain insight into the caspase re...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20050018
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND INFORMATION:In eukaryotic cells, autophagy is considered a lysosomal catabolic process which participates in the degradation of intracellular components in a vacuolar structure termed autolysosome. This pathway plays a significant role in the erythropoiesis process, contributing to the clearance of some orga...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201500058
更新日期：2016-04-01 00:00:00
abstract：:A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rathe...
journal_title：Biology of the cell
pub_type： 历史文章,杂志文章,评审
doi：10.1016/s0248-4900(03)00078-9
更新日期：2003-09-01 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:Interaction of sperm and egg at fertilization induces well-coordinated molecular events including specific recognition between species, adhesion and fusion, that lead to the formation of a zygote, a totipotent cell that develops into a new individual. A calcium signal, common to a great number of species, from marine ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01065-0
更新日期：2000-07-01 00:00:00
abstract：:Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form dom...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(94)80004-9
更新日期：1994-01-01 00:00:00
abstract：:A quantitative ultrastructural study was performed on 56 ejaculates showing anomalies of the sperm axonemal complex. The anomalies comprised either the absence of one, or more often several, axonemal structures, or defective elongation of the doublets. Several characteristics relating to the extent and superimposition...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00253.x
更新日期：1984-01-01 00:00:00
abstract：:Brain intricacies and the difficulty that scientists encounter in revealing its function with standard approaches such as electrical stimulation of neurons have led to the exploration of new tools that enable the study of neural circuits in a remote and non-invasive way. To this end, optogenetics has initialised a rev...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201200087
更新日期：2013-10-01 00:00:00
abstract：:This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Our knowledge of cell signalling pathways in the molluscan immune system and their response to immunological challenge is currently poor. The present study focused on the Protein Kinase C (PKC) pathway in the immune cells (haemocytes) of Lymnaea stagnalis and its response following exposure to bacterial lipopolysaccha...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/j.biolcel.2003.09.001
更新日期：2003-11-01 00:00:00
abstract：:The diversity of microtubular networks was analyzed in quail oviduct and in Paramecium cells using conventional and confocal immunofluorescence as well as pre- and post-embedding EM immunocytochemistry with a variety of anti-tubulin antibodies. The 6-11B-1 monoclonal antibody, specific for the post-translational acety...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(91)90069-y
更新日期：1991-01-01 00:00:00
abstract：:Image analysis was used to study the repair process of a circular mechanical lesion of confluent human endothelial cells in culture after irradiation (10 Gy) prior to wounding. Coverage of denuded areas 48 and 96 h after injury of endothelial cells was identical in control and irradiated cultures, although the labelin...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90313-r
更新日期：1990-01-01 00:00:00
abstract：:cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90134-m
更新日期：1992-01-01 00:00:00
