Bacterial lipopolysaccharide modulates protein kinase C signalling in Lymnaea stagnalis haemocytes.

Abstract:

:Our knowledge of cell signalling pathways in the molluscan immune system and their response to immunological challenge is currently poor. The present study focused on the Protein Kinase C (PKC) pathway in the immune cells (haemocytes) of Lymnaea stagnalis and its response following exposure to bacterial lipopolysaccharide (LPS). Western blotting of haemocyte proteins with either anti-PKC (pan) or anti-phospho-PKC (Ser 660) antibodies revealed the presence of two PKC-like immuno-reactive proteins of approximately 76 and 85 kDa. Challenge of haemocytes with LPS transiently increased the phosphorylation of the 85 kDa isoform, with a 2.2-fold increase in phosphorylation levels at 5 min and a return to basal levels after 20 min. This LPS-mediated response was blocked following treatment of haemocytes with GF109203X. PKC activities measured in anti-phospho-PKC immunocomplexes following haemocyte treatment with LPS and GF109203X correlated well with the observed PKC phosphorylation levels. These data show for the first time that the activity of the PKC pathway in molluscan immune cells is modulated by LPS, as it is in mammals, and suggest that cell signalling in the innate immune response may have been conserved through evolution.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Walker AJ,Plows LD

doi

10.1016/j.biolcel.2003.09.001

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

527-33

issue

8

eissn

0248-4900

issn

1768-322X

pii

S0248490003001199

journal_volume

95

pub_type

杂志文章
  • Stimulation by aldosterone of a conductive chloride pathway in toad skin.

    abstract::As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Crabbe J,Beaujean V,Devuyst O

    更新日期:1989-01-01 00:00:00

  • Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry.

    abstract::Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction)...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00402.x

    authors: Barni S,Gerzeli G

    更新日期:1985-01-01 00:00:00

  • Looking for nuclear translation using xenopus oocytes.

    abstract::In eukaryotes transcription and translation takes place in two different compartments separated by the nuclear envelope. Nuclear translation has been reported in several publications, however, all these published results have been criticized, particularly with respect to cytoplasmic contamination of the nuclei. We hav...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(03)00059-5

    authors: Cosson B,Philippe M

    更新日期:2003-07-01 00:00:00

  • Monoclonal antibody against a nuclear matrix antigen in proliferating human cells.

    abstract::A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00539.x

    authors: Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AA

    更新日期:1987-01-01 00:00:00

  • Trafficking and phosphorylation dynamics of AQP4 in histamine-treated human gastric cells.

    abstract:BACKGROUND INFORMATION:AQP4 (aquaporin 4) internalization and a concomitant decrease in the osmotic water permeability coefficient (Pf) after histamine exposure has been reported in AQP4-transfected gastric HGT1 cells. RESULTS:In the present study we report that AQP4 internalization is followed by an increase in AQP4 ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20060068

    authors: Carmosino M,Procino G,Tamma G,Mannucci R,Svelto M,Valenti G

    更新日期:2007-01-01 00:00:00

  • Benzoxazinone derivatives: new fluorescent probes for two-color flow cytometry analysis using one excitation wavelength.

    abstract::A new class of fluorescent dye which upon excitation at 488 nm turns red is shown to be probe-suitable for using in flow cytometry alone or in conjunction with fluorescein derivatives. 7-dimethylamino 3-(p-formylstyryl) 1,4 benzoxazin 2-one is suitable for rendering microorganisms, such as Plasmodium merozoites and ce...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Monsigny M,Midoux P,Le Bris MT,Roche AC,Valeur B

    更新日期:1989-01-01 00:00:00

  • Dopamine-induced programmed cell death is associated with cytochrome c release and caspase-3 activation in snail salivary gland cells.

    abstract:BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070168

    authors: Pirger Z,Rácz B,Kiss T

    更新日期:2009-02-01 00:00:00

  • Microspore-derived embryogenesis in pepper (Capsicum annuum L.): subcellular rearrangements through development.

    abstract::Background information. In vitro-cultured microspores, after an appropriate stress treatment, can switch towards an embryogenic pathway. This process, known as microspore embryogenesis, is an important tool in plant breeding. Basic studies on this process in economically interesting crops, especially in recalcitrant p...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040142

    authors: Bárány I,González-Melendi P,Fadón B,Mitykó J,Risueño MC,Testillano PS

    更新日期:2005-09-01 00:00:00

  • VICKZ proteins: a multi-talented family of regulatory RNA-binding proteins.

    abstract::The highly conserved VICKZ (Vg1 RBP/Vera, IMP-1,2,3, CRD-BP, KOC, ZBP-1) family of RNA-binding proteins recognize specific cis-acting elements in a variety of different RNAs and have been implicated in cell polarity and migration, cell proliferation, and cancer. In just the last two years, the use of transgenic mice, ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20040151

    authors: Yisraeli JK

    更新日期:2005-01-01 00:00:00

  • Action of photoreactive analogs of vasopressin in toad bladder.

    abstract::A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00431.x

    authors: Eggena P,Ma CL,Fahrenholz F,Buku A,Schwartz IL

    更新日期:1985-01-01 00:00:00

  • A morphometric study of subcellular responses in frog pituitary corticotropic cells to constant environmental conditions.

    abstract::Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1986.tb00472.x

    authors: Gracia-Navarro F,Garcia-Navarro S,Garcia-Herdugo G

    更新日期:1986-01-01 00:00:00

  • Cryoelectron microscopy of macromolecular complexes.

    abstract::Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X-ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies. It is now clear that a powerful approach is t...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/j.1768-322x.1994.tb00932.x

    authors: Wade RH,Hewat EA

    更新日期:1994-01-01 00:00:00

  • Centriole and basal body formation during ciliogenesis revisited.

    abstract::This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/0248-4900(91)90075-x

    authors: Dirksen ER

    更新日期:1991-01-01 00:00:00

  • Structural and immunohistological modifications in olfactory bulb of the staggerer mutant mouse.

    abstract::In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Monnier Z,Bahjaoui-Bouhaddi M,Bride J,Bride M,Math F,Propper A

    更新日期:1999-01-01 00:00:00

  • High molecular weight microtubule-associated proteins from pig brain are immunologically related to human erythrocyte membrane proteins spectrin, ankyrin, proteins 4.1 and 4.2.

    abstract::The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00361.x

    authors: Hill AM,Cassoly R,Chetrite G,Pantaloni D

    更新日期:1985-01-01 00:00:00

  • Microtubule diversity in ciliated cells: evidence for its generation by post-translational modification in the axonemes of Paramecium and quail oviduct cells.

    abstract::The diversity of microtubular networks was analyzed in quail oviduct and in Paramecium cells using conventional and confocal immunofluorescence as well as pre- and post-embedding EM immunocytochemistry with a variety of anti-tubulin antibodies. The 6-11B-1 monoclonal antibody, specific for the post-translational acety...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90069-y

    authors: Adoutte A,Delgado P,Fleury A,Levilliers N,Lainé MC,Marty MC,Boisvieux-Ulrich E,Sandoz D

    更新日期:1991-01-01 00:00:00

  • Presence of aquaporin and V-ATPase on the contractile vacuole of Amoeba proteus.

    abstract:BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070091

    authors: Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

    更新日期:2008-03-01 00:00:00

  • Characterization and biological properties of L-HGP, a glycoprotein from the amphibian oviduct with acrosome-stabilizing effects.

    abstract:BACKGROUND INFORMATION:The role of the jelly coat that surrounds the amphibian oocytes has been widely discussed, but is poorly understood. The presence of the jelly coat is essential for fertilization. However, the structure and function of the molecules that comprise the jelly coat have not been thoroughly documented...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20050051

    authors: Krapf D,Vidal M,Arranz SE,Cabada MO

    更新日期:2006-07-01 00:00:00

  • Stress response in Drosophila subobscura: DNA-RNA hybrids and transcriptional activity.

    abstract::Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90140-v

    authors: Arbona M,Cuenca JB,de Frutos R

    更新日期:1992-01-01 00:00:00

  • Apical membrane marker is expressed early in colonic epithelial cells.

    abstract::We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00557.x

    authors: Le Bivic A,Hirn M,Reggio H

    更新日期:1987-01-01 00:00:00

  • Acute changes in temperature or oxygen availability induce ROS fluctuations in Daphnia magna linked with fluctuations of reduced and oxidized glutathione, catalase activity and gene (haemoglobin) expression.

    abstract:BACKGROUND INFORMATION:ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20100145

    authors: Becker D,Brinkmann BF,Zeis B,Paul RJ

    更新日期:2011-08-01 00:00:00

  • The orientation of ciliary basal bodies in quail oviduct is related to the ciliary beating cycle commencement.

    abstract::In mature ciliated cells, the basal feet associated to the basal bodies point out in the direction of the effective stroke of the ciliary beating. In contrast, during ciliogenesis, the basal feet of the newly anchored basal bodies are randomly oriented. The reorientation of basal bodies occurs during the beginning of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00417.x

    authors: Boisvieux-Ulrich E,Laine MC,Sandoz D

    更新日期:1985-01-01 00:00:00

  • Densitometric analysis of nuclear DNA content in lentil roots grown in space.

    abstract::Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(94)90055-8

    authors: Driss-Ecole D,Schoevaert D,Noin M,Perbal G

    更新日期:1994-01-01 00:00:00

  • Length of the selectivity filter of aquaporin-1.

    abstract::We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Whittembury G,González E,Gutiérrez AM,Echevarría M,Hernández CS

    更新日期:1997-08-01 00:00:00

  • Is fat so bad? Modulation of endoplasmic reticulum stress by lipid droplet formation.

    abstract::LDs (lipid droplets) have long been considered as inert particles used by the cells to store fatty acids and sterols as esterified non-toxic lipid species (i.e. triacylglycerols and steryl esters). However, accumulating evidence suggests that LDs behave as a dynamic compartment, which is involved in the regulation of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20100144

    authors: Hapala I,Marza E,Ferreira T

    更新日期:2011-06-01 00:00:00

  • From progesterone to active Cdc2 in Xenopus oocytes: a puzzling signalling pathway.

    abstract::Since almost two decades, it is known that progesterone is responsible of the release of the prophase I arrest of amphibian oocytes and leads to the activation of the universal MPF, through a puzzling transduction pathway. It involves negative regulation of the cAMP-dependent protein kinase (PKA) and synthesis of new ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(01)01126-1

    authors: Karaiskou A,Dupré A,Haccard O,Jessus C

    更新日期:2001-09-01 00:00:00

  • Construction and characterization of a DNA library from mouse chromosomes 19 purified by flow cytometry.

    abstract::In spite of the constant development concerning physical mapping of eukaryotic genomes, the mouse chromosome 19 remains poorly characterized. In order to improve the possibilities for studying this chromosome, we have constructed a chromosome-specific EcoRI DNA fragment library from mouse chromosomes 19 sorted by flow...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90322-t

    authors: Baron B,Métézeau P,Kiefer-Gachelin H,Goldberg ME

    更新日期:1990-01-01 00:00:00

  • Compartmentalized DCC signalling is distinct from DCC localized to lipid rafts.

    abstract:BACKGROUND INFORMATION:Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070108

    authors: Petrie RJ,Zhao B,Bedford F,Lamarche-Vane N

    更新日期:2009-02-01 00:00:00

  • Mapping the cellular distribution of labelled molecules by SIMS microscopy.

    abstract::We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90011-o

    authors: Hindie E,Coulomb B,Beaupain R,Galle P

    更新日期:1992-01-01 00:00:00

  • The lateral line of zebrafish: a model system for the analysis of morphogenesis and neural development in vertebrates.

    abstract::The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2003.10.005

    authors: Dambly-Chaudière C,Sapède D,Soubiran F,Decorde K,Gompel N,Ghysen A

    更新日期:2003-12-01 00:00:00