Abstract：
:As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incubating amphibian skin (mostly Bufo marinus) with Cl- present on the epithelial side only, and after blocking JNa+ by combined treatment with ouabain and amiloride. In such conditions, JCl- was found to be equal to (reversed) Isc; furthermore, when JCl- was "translated" in terms of conductance, gCl-, the latter accounted almost quantitatively for transepithelial conductance, g1. When residual intratissue (i.e. intracellular) electronegativity was eliminated by replacing Na+ with K+, JCl- was larger but Isc and JCl- were still found to reflect each other, and gCl- again accounted for most, if not all, of g1. JCl- in the opposite direction, as a result of Cl- being present only on the dermal side, was negligible, and g1 was very low. Thus, in the absence of sodium transport, when experimental conditions are such that a net inward JCl- obtains, the anion apparently flows only through (a) conductive pathway(s). Aldosterone is probably involved in the regulation of this pathway, as JCl- was much lower when toads were maintained in dilute saline than in water or on moist peat; so was the fraction of the apical surface corresponding to mitochondria-rich cells.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Crabbe J,Beaujean V,Devuyst Osubject
Has Abstractpub_date
1989-01-01 00:00:00pages
173-7issue
1-2eissn
0248-4900issn
1768-322Xjournal_volume
66pub_type
杂志文章abstract：:HT 29-D4 is a clonal cell line, derived from the human colon adenocarcinoma cell line HT 29, which can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (Fantini et al. [1986], J. Cell Sci. 83, 235-249). Both undifferentiated and differentiated HT 29-D4 ce...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201900085
更新日期：2020-05-01 00:00:00
abstract：:We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00557.x
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Caspase-dependent and -independent death mechanisms are involved in apoptosis in a variety of human carcinoma cells treated with antineoplastic compounds. Our laboratory has reported that p53 is a key contributor of mitochondrial apoptosis in cervical carcinoma cells after staurosporine exposure....
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080164
更新日期：2009-06-04 00:00:00
abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90066-3
更新日期：1994-01-01 00:00:00
abstract：:The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040515
更新日期：2005-05-01 00:00:00
abstract：:Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluo...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90129-3
更新日期：1993-01-01 00:00:00
abstract：:We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(02)01220-0
更新日期：2003-01-01 00:00:00
abstract：:Although much of eukaryotic cell biology is highly conserved, the most biomedically relevant organisms are often the most difficult or expensive to study. Non-vertebrate model organisms offer a practical and accessible alternative. Large collections of loss-of-function mutants have now been generated in the nematode C...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070005
更新日期：2007-06-01 00:00:00
abstract：:Ciliary receptor cells, typified by cilia or modified cilia, are very common in the animal kingdom. In addition to the cytoskeleton of their ciliary processes these receptors possess other specific prominent cytoskeletal elements. Two representative systems are presented: i) scolopidia, mechanosensitive sensilla of va...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90441-3
更新日期：1992-01-01 00:00:00
abstract：:Peroxisomes are present in most eukaryotic cell types, and have different enzymatic content and metabolic functions throughout the life scale. The endosymbiotic origin of these DNA-devoid organelles is supported by evolutionary data concerning genes encoding not only most peroxisomal proteins, but also several transcr...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01083-2
更新日期：2000-09-01 00:00:00
abstract：:The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1989.tb00867.x
更新日期：1989-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060012
更新日期：2006-10-01 00:00:00
abstract：:Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20050058
更新日期：2006-03-01 00:00:00
abstract：:For the purpose of investigating the nature of the nervous factor which controls cell proliferation in limb blastema of Newts, we have cultured primary mesenchymous cells from limb blastemas of Axolotl. The cultures were carried out in Petri dishes (Primaria, Falcon) with a basal medium with contained diluted MEM supp...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00512.x
更新日期：1986-01-01 00:00:00
abstract：:Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stag...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00448.x
更新日期：1986-01-01 00:00:00
abstract：:In many cell types, microtubules are preferentially associated with the Golgi apparatus. However, the existence of a functional link between these two organelles is still hypothetical. To gain insight into this question, the relationships between microtubules and the Golgi apparatus were studied in rat hepatocytes dur...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00222.x
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090098
更新日期：2010-02-17 00:00:00
abstract：BACKGROUND INFORMATION:Carcinoma of the oesophagus is the sixth leading cause of cancer death in the western world and is associated with a 5-year survival of less than 15%. Recent evidence suggests that stromal-epithelial interactions are fundamental in carcinogenesis. The advent of co-culture techniques permits the i...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20100071
更新日期：2010-12-01 00:00:00
abstract：:Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The nucleolar lesions provoked by the action of ribonuclease (RNase) on living chick embryo fibroblasts were studied by means of microcinematographic analysis and at the ultrastructural level using oxidized diaminobenzidine as a differentially contrasting stain for nucleic acids. This study has shown that the inductio...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00218.x
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND INFORMATION:ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20100145
更新日期：2011-08-01 00:00:00
abstract：:We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90011-o
更新日期：1992-01-01 00:00:00
abstract：:The micromeres, the first cells to be specified in sea urchin embryos, are generated by unequal cleavage at the fourth cell division. The micromeres differentiate autonomously to form spicules and dispatch signals to induce endomesoderm in the neighbouring macromeres cells in the embryo. Using a calcium indicator Fura...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/j.biolcel.2003.11.009
更新日期：2004-03-01 00:00:00
abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00539.x
更新日期：1987-01-01 00:00:00
abstract：:Apoptotic-like events could be rapidly induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in cells of the mouse plasmacytoma cell line MPC-11 grown in serum-free medium. Indicators for apoptosis were morphological changes visualized by light and electron microscopy, such as chromatin condensation ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND:Integrins are transmembrane alphabeta heterodimer receptors that function as structural and functional bridges between the cytoskeleton and ECM (extracellular matrix) molecules. The RGD (arginine-glycine-aspartate tripeptide motif)-dependent integrin alpha8beta1 has been shown to be involved in various cell ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090060
更新日期：2009-09-14 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:Tensins are focal adhesion molecules that were identified and characterised in the late 1980s to early 1990s. They play an essential role in the control of cell adhesion. Tensins can bind the tail of ß integrin via their phospho tyrosine binding domain, they exhibit various protein interaction domains including a Src ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201600053
更新日期：2017-03-01 00:00:00
