Abstract:
:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (pHe) rapidly reduced the intracellular pH (pHi) in CHO cells. Lowering the pHi to near the neutral range significantly accelerated the growth of VLB-induced paracrystals, compared to that of paracrystals formed at a physiological pHe. However, further cytoplasmic acidification caused by the addition of sodium azide into the culture medium induced the disappearance of typical paracrystals and the appearance of a highly organized meshwork of tubulin appearing as short, thick filaments at the light microscopic level. Treatments using different concentrations of VLB at different pHe's showed that low pHi's (6.7 and 6.3) suppressed paracrystal-formation at lower concentrations of VLB (5 x 10(-6) M and 10(-5) M). At higher concentrations of VLB (5 x 10(-5) M and 10(-4) M), only short filaments were formed at pHi 6.3. Electron microscopy revealed that the filaments had a ladder-like structure probably consisting of a stacked series of fused rings. This indicates that paracrystals may be modified by extremely low pH. These results show that paracrystals are unstable in living cells and that their formation is regulated by environmental pH.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Takanari H,Morita J,Yamanaka H,Yada K,Takahashi A,Izutsu Kdoi
10.1016/0248-4900(94)90066-3subject
Has Abstractpub_date
1994-01-01 00:00:00pages
51-7issue
1eissn
0248-4900issn
1768-322Xpii
0248-4900(94)90066-3journal_volume
82pub_type
杂志文章abstract::Image analysis was used to study the repair process of a circular mechanical lesion of confluent human endothelial cells in culture after irradiation (10 Gy) prior to wounding. Coverage of denuded areas 48 and 96 h after injury of endothelial cells was identical in control and irradiated cultures, although the labelin...
journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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更新日期:1999-01-01 00:00:00
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更新日期:2010-12-01 00:00:00
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journal_title:Biology of the cell
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