Abstract：
:The p126 protein is synthesized by P. falciparum between the 32nd and the 36th hour of the erythrocytic cycle, and is localized in the parasitophorous vacuole. It is processed when schizonts rupture and the major fragments (50, 47 and 18 kDa), which are released into culture supernatant, have been characterized using monoclonal antibodies. The 47 kDa fragment has been mapped at the N-terminus of the molecule. The portion of the protein p126 gene coding for this fragment contains 3 introns and is characterized by a sequence coding for 6 repeats of 8 aminoacids and by repeats of TCA/T-AGT coding for a polyserine sequence of 37 serines in a row for the FCR-3 strain. The 50 kDa fragment is also found in culture supernatant when merozoites are released from mature schizonts. The incubation of mature schizonts with leupeptin inhibits the release of merozoites and, in this case, a 56 kDa intermediate product is found. In those conditions, merozoites were observed free in the erythrocyte cytoplasm, the membrane of the parasitophorous vacuole being destroyed. The 50 kDa fragment can be obtained from the 56 kDa fragment by treatment with trypsin (a protease inhibited by leupeptin). Our results suggest that the processing of the 56 kDa fragment: 1) is protease-dependent, and could depend on a trypsin-like activity; 2) cannot occur after the release of merozoites because of the protease inhibitors contained in the serum; 3) does not occur before the release of merozoites, since no processed products of the protein p126 are observed in unruptured schizonts.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biol Celljournal_title
Biology of the cellauthors
Delplace P,Bhatia A,Cagnard M,Camus D,Colombet G,Debrabant A,Dubremetz JF,Dubreuil N,Prensier G,Fortier Bdoi
10.1016/0248-4900(88)90080-9subject
Has Abstract,Author List Incompletepub_date
1988-01-01 00:00:00pages
215-21issue
2eissn
0248-4900issn
1768-322Xjournal_volume
64pub_type
杂志文章abstract：:This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(91)90075-x
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Carcinoma of the oesophagus is the sixth leading cause of cancer death in the western world and is associated with a 5-year survival of less than 15%. Recent evidence suggests that stromal-epithelial interactions are fundamental in carcinogenesis. The advent of co-culture techniques permits the i...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20100071
更新日期：2010-12-01 00:00:00
abstract：:As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:In many cell types, microtubules are preferentially associated with the Golgi apparatus. However, the existence of a functional link between these two organelles is still hypothetical. To gain insight into this question, the relationships between microtubules and the Golgi apparatus were studied in rat hepatocytes dur...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00222.x
更新日期：1983-01-01 00:00:00
abstract：:I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:Recent advances in understanding the in vitro translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane have established a molecular basis for the initial reactions predicted by the signal hypothesis. The first two events involve a transient arrest of nascent chain elongation, followed by a ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1985.tb00319.x
更新日期：1984-01-01 00:00:00
abstract：:Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00577.x
更新日期：1987-01-01 00:00:00
abstract：:Bistramide A, a new toxin isolated from a New Caledonian Urochordata, shows an antiproliferative effect on a non-small-cell lung carcinoma line in vitro and G1-blockade. In this work, the growth arrest induced by bistramide A was shown to be irreversible as assessed by growth kinetics of pretreated cells. Furthermore,...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(05)80196-0
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Death receptors (DRs) induce intracellular signalling upon engagement of their cognate ligands, leading to apoptosis, cell survival or pro-inflammatory responses. In mammals, DR signalling is mediated by the recruitment of several DD (death domain)-containing molecules, such as FADD (Fas-associat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20050091
更新日期：2006-08-01 00:00:00
abstract：BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201900085
更新日期：2020-05-01 00:00:00
abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90066-3
更新日期：1994-01-01 00:00:00
abstract：:The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90129-3
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Human gingival tissues are prone to hyperplasia under inflammatory stimuli. We have identified gingival tissue-specific mesenchymal stem cells (GMSCs) and found their functional change being correlated with drug-induced gingival hyperplasia. However, whether these cells exhibit characteristics of...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201200064
更新日期：2013-06-01 00:00:00
abstract：:Our recent data suggest that during auto- and allograft recognition in sponges (Porifera), cytokines are differentially expressed. Since the mitogen-activated protein kinase (MAPK) signal transduction modulates the synthesis and release of cytokines, we intended to identify one key molecule of this pathway. Therefore,...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(00)89017-6
更新日期：2000-04-01 00:00:00
abstract：:The aim of a large number of studies on G protein-coupled receptors was centered on understanding the structural basis of their main functional properties. Here, we will briefly review the results obtained on the alpha1-adrenergic receptor subtypes belonging to the rhodopsin-like family of receptors. These findings co...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/j.biolcel.2004.03.009
更新日期：2004-06-01 00:00:00
abstract：BACKGROUND INFORMATION:CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090096
更新日期：2009-12-04 00:00:00
abstract：:Interactions of cells with the extracellular matrix are essential for the control of tissue remodelling, cell migration, and embryogenesis. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where transmembrane adhesion receptors provide a structural link bet...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01101-1
更新日期：2000-10-01 00:00:00
abstract：:In the present study we report the occurrence of chaperonins, cpn10 and cpn60, in Chromatium vinosum and rat hepatocytes, using specific polyclonal antibodies in conjunction with the protein A-gold immunocytochemical technique. As demonstrated by quantitative evaluations, the immunolabeling for cpn10 and cpn60 in C vi...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1995.tb00943.x
更新日期：1995-01-01 00:00:00
abstract：:The centrosome of the slime mould Dictyostelium discoideum displays a morphology markedly different from centriolar centrosomes or yeast spindle pole bodies, while fulfilling the same conserved functions in the organization of the microtubule cytoskeleton. Recent advances suggest that the Dictyostelium centrosome may ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(99)80092-6
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201600050
更新日期：2017-06-01 00:00:00
abstract：:The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(97)89840-1
更新日期：1996-01-01 00:00:00
abstract：:PD (peritoneal dialysis) is an established mode of renal replacement therapy, based on the exchange of fluid and solutes between blood in peritoneal capillaries and a dialysate that has been introduced into the peritoneal cavity. The dialysis process involves diffusive and convective transports and osmosis through the...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040132
更新日期：2005-09-01 00:00:00
abstract：:Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stag...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00448.x
更新日期：1986-01-01 00:00:00
abstract：:Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00574.x
更新日期：1987-01-01 00:00:00
abstract：:The highly conserved VICKZ (Vg1 RBP/Vera, IMP-1,2,3, CRD-BP, KOC, ZBP-1) family of RNA-binding proteins recognize specific cis-acting elements in a variety of different RNAs and have been implicated in cell polarity and migration, cell proliferation, and cancer. In just the last two years, the use of transgenic mice, ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040151
更新日期：2005-01-01 00:00:00
abstract：:The occurrence of the tubulin post-translational modification, polyglycylation, in stable microtubular structures was investigated during morphogenesis in two ciliates, Paramecium and Frontonia atra, belonging to the Epiplasmata group. This analysis was carried out by means of immunofluorescence and post-embedding imm...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(00)01107-2
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND INFORMATION:Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070108
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND INFORMATION:Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nuc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080054
更新日期：2009-03-01 00:00:00
abstract：BACKGROUND INFORMATION:The role of the jelly coat that surrounds the amphibian oocytes has been widely discussed, but is poorly understood. The presence of the jelly coat is essential for fertilization. However, the structure and function of the molecules that comprise the jelly coat have not been thoroughly documented...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20050051
更新日期：2006-07-01 00:00:00
abstract：BACKGROUND INFORMATION:PrAO (primary amine oxidase), also known as SSAO (semicarbazide-sensitive amine oxidase)/VAP-1 (vascular adhesion protein-1), is an enzyme (EC 1.4.3.21) that is highly expressed in blood vessels and participates in many cell processes, including glucose handling or inflammatory leucocyte recruitm...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20110049
更新日期：2011-11-01 00:00:00
