Identification of novel adhesion proteins in mouse peritoneal macrophages.

abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

journal_title：Biology of the cell

authors： Rebelo L,Carmo-Fonseca M,Moura TF

更新日期：1992-01-01 00:00:00

abstract：:The PAS-positive-calcium-sensitive (Ca-s) cells of the pars intermedia (PI) were studied in goldfish kept in fresh water (FW), deionized water (DW), 1/3 sea water (SW) and 1/3 Ca-free SW. Ultrastructural studies show that Ca-s cells of control goldfish kept in FW have a low activity with elongated or deeply indented n...

journal_title：Biology of the cell

authors： Olivereau M,Chambolle P,Dubourg P

更新日期：1983-01-01 00:00:00

abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...

journal_title：Biology of the cell

authors： Denefle JP,Zhu QL,Lechaire JP

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nuc...

journal_title：Biology of the cell

authors： Storck S,Thiry M,Bouvet P

更新日期：2009-03-01 00:00:00

abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....

journal_title：Biology of the cell

authors： Tassin AM,Bornens M

更新日期：1999-05-01 00:00:00

abstract：:HT 29-D4 is a clonal cell line, derived from the human colon adenocarcinoma cell line HT 29, which can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (Fantini et al. [1986], J. Cell Sci. 83, 235-249). Both undifferentiated and differentiated HT 29-D4 ce...

journal_title：Biology of the cell

authors： Fantini J,Verrier B,Marvaldi J,Mauchamp J

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND INFORMATION:αvβ5 integrin and Mer tyrosine kinase (MerTK) receptors reside at the apical surface of the retinal pigment epithelium (RPE) in the eye to promote the diurnal, synchronised phagocytosis of shed photoreceptor outer segment fragments (POS) that is critical for vision. Phagocytosis assays studying R...

journal_title：Biology of the cell

authors： Nandrot EF,Silva KE,Scelfo C,Finnemann SC

更新日期：2012-06-01 00:00:00

abstract：:Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...

journal_title：Biology of the cell

authors： Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

更新日期：1988-01-01 00:00:00

abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...

journal_title：Biology of the cell

authors： Tronchin G,Bouchara JP,Larcher G,Lissitzky JC,Chabasse D

更新日期：1993-01-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Proliferating cell nuclear antigen (PCNA) is a key component of the DNA replication machinery involved in the process of DNA elongation, recombination, methylation and repair. We have used PCNA fused with green fluorescent protein (GFP-PCNA) as a convenient tool to show the progress of S-phase in...

journal_title：Biology of the cell

authors： Kisielewska J,Lu P,Whitaker M

更新日期：2005-03-01 00:00:00

abstract：:We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...

journal_title：Biology of the cell

authors： Whittembury G,González E,Gutiérrez AM,Echevarría M,Hernández CS

更新日期：1997-08-01 00:00:00

abstract：:We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...

journal_title：Biology of the cell

authors： Hindie E,Coulomb B,Beaupain R,Galle P

更新日期：1992-01-01 00:00:00

abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...

journal_title：Biology of the cell

authors： Quemener V,Moulinoux JP,Khan NA,Seiler N

更新日期：1990-01-01 00:00:00

abstract：:We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10(-8) M ouabain, the number of domes seen on Caco-2 cell monolayers grown on plastic dishes wa...

journal_title：Biology of the cell

authors： Ramond MJ,Martinot-Peignoux M,Erlinger S

更新日期：1985-01-01 00:00:00

abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...

journal_title：Biology of the cell

authors： Takanari H,Morita J,Yamanaka H,Yada K,Takahashi A,Izutsu K

更新日期：1994-01-01 00:00:00

abstract：:Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...

journal_title：Biology of the cell

authors： Quintana C,Olmedilla A,Antoine N,Ollacarizqueta A

更新日期：1987-01-01 00:00:00

abstract：:In eukaryotes transcription and translation takes place in two different compartments separated by the nuclear envelope. Nuclear translation has been reported in several publications, however, all these published results have been criticized, particularly with respect to cytoplasmic contamination of the nuclei. We hav...

journal_title：Biology of the cell

authors： Cosson B,Philippe M

更新日期：2003-07-01 00:00:00

abstract：:A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rathe...

journal_title：Biology of the cell

authors： Amos WB,White JG

更新日期：2003-09-01 00:00:00

abstract：BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...

journal_title：Biology of the cell

authors： Zimowska M,Swierczynska M,Ciemerych MA

更新日期：2013-08-01 00:00:00

abstract：:Toad bladder epithelial cells were homogenized and fractionated by Percoll density-gradient centrifugation. Binding of tritium-labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60-min) microsomal supernatant fraction (S). More than two-thirds of the total receptors ...

journal_title：Biology of the cell

authors： Eggena P,Ma CL

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND INFORMATION:AQP4 (aquaporin 4) internalization and a concomitant decrease in the osmotic water permeability coefficient (Pf) after histamine exposure has been reported in AQP4-transfected gastric HGT1 cells. RESULTS:In the present study we report that AQP4 internalization is followed by an increase in AQP4 ...

journal_title：Biology of the cell

authors： Carmosino M,Procino G,Tamma G,Mannucci R,Svelto M,Valenti G

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...

journal_title：Biology of the cell

authors： Buschke DG,Squirrell JM,Fong JJ,Eliceiri KW,Ogle BM

更新日期：2012-06-01 00:00:00

abstract：:We used a high-resolution STEM to attempt to recognize the spatial localization of the macromolecular components of ribosomes isolated from Tetrahymena pyriformis. Images obtained either by the annular dark field detector or by the ratio-contrast mode (Z-contrast) show a ribosome as constituted of rounded dark areas, ...

journal_title：Biology of the cell

authors： Curgy JJ,Iftode F,Perasso R,André J,Mory C,Colliex C

更新日期：1984-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...

journal_title：Biology of the cell

authors： Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

更新日期：2008-03-01 00:00:00

abstract：BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...

journal_title：Biology of the cell

authors： Jablonski EM,Hughes FM Jr

更新日期：2006-01-01 00:00:00

abstract：:Cilia from quail oviduct were isolated with their membrane. The ultrastructural study revealed a good preservation of cilia in the purified fraction. Electrophoresis on SDS-PAGE showed a reproducible pattern of ciliary proteins, the major bands being those of tubulins 57 kDa and dyneins above 250 kDa. Among the minor ...

journal_title：Biology of the cell

authors： Chailley B,Bork K,Gounon P,Sandoz D

更新日期：1986-01-01 00:00:00

abstract：:The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...

journal_title：Biology of the cell

authors： Mattei MG,Duprey P,Li ZL,Mattei JF,Paulin D

更新日期：1989-01-01 00:00:00

abstract：:beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...

journal_title：Biology of the cell

authors： Gavard J,Mège RM

更新日期：2005-12-01 00:00:00

abstract：:The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to sync...

journal_title：Biology of the cell

authors： Helenius A

更新日期：1984-01-01 00:00:00