Looking for nuclear translation using xenopus oocytes.

Abstract:

:In eukaryotes transcription and translation takes place in two different compartments separated by the nuclear envelope. Nuclear translation has been reported in several publications, however, all these published results have been criticized, particularly with respect to cytoplasmic contamination of the nuclei. We have investigated the question of nuclear protein synthesis using a different strategy. We microinjected frog oocytes with different DNA and RNA constructs and searched for translation products in nuclei separated from the cytoplasm by microdissection. We have been unable to detect any translation within the nuclei but rather a saturable translation in the perinuclear cytoplasm associated with the isolated nuclei.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Cosson B,Philippe M

doi

10.1016/s0248-4900(03)00059-5

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

321-5

issue

5

eissn

0248-4900

issn

1768-322X

pii

S0248490003000595

journal_volume

95

pub_type

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