Anti-fibronectin serum inhibits the disorganization of the dermal-epidermal junction in cultured wounded skin.

Abstract:

:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum germinativum cells protruding towards the dermis and in their marginal regions (membrane-associated plaques). Faint staining was visible at the level of the lamina densa and inside some parts of the lamina lucida. In comparison, contrasted ultrathin sections revealed classical disorganization of the dermal-epidermal junction. In the presence of anti-fibronectin serum during the whole time of culture, fibronectin-antifibronectin binding was visualized in the form of sparse cytoplasmic granules in the epidermal processes of the stratum germinativum cells. Contrasted ultrathin sections emphasized the continuity between the tonofilaments, the anchoring filaments and the anchoring fibrils. Briefly, anti-fibronectin serum inhibits the disorganization of the dermal-epidermal junction in cultured wounded skin.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Denefle JP,Zhu QL,Lechaire JP

doi

10.1111/j.1768-322x.1989.tb03013.x

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

91-5

issue

1

eissn

0248-4900

issn

1768-322X

journal_volume

67

pub_type

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