Atomic force microscopy and modeling of natural elastic fibrillin polymers.

abstract：:Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...

journal_title：Biology of the cell

authors： Migné C,Prensier G,Cornu A,Grenet E

更新日期：1996-01-01 00:00:00

abstract：:Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due ...

journal_title：Biology of the cell

authors： Roingeard P,Hourioux C,Blanchard E,Brand D,Ait-Goughoulte M

更新日期：2004-03-01 00:00:00

abstract：:Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...

journal_title：Biology of the cell

authors： Zini N,Scotlandi K,Baldini N,Nini G,Sabatelli P,Maraldi N

更新日期：1995-01-01 00:00:00

abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...

journal_title：Biology of the cell

authors： Palluault F,Dei-Cas E,Slomianny C,Soulez B,Camus D

更新日期：1990-01-01 00:00:00

abstract：:A new class of fluorescent dye which upon excitation at 488 nm turns red is shown to be probe-suitable for using in flow cytometry alone or in conjunction with fluorescein derivatives. 7-dimethylamino 3-(p-formylstyryl) 1,4 benzoxazin 2-one is suitable for rendering microorganisms, such as Plasmodium merozoites and ce...

journal_title：Biology of the cell

authors： Monsigny M,Midoux P,Le Bris MT,Roche AC,Valeur B

更新日期：1989-01-01 00:00:00

abstract：:The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the laye...

journal_title：Biology of the cell

authors： Kattenbach WM,Diniz Junior JA,Benchimol M,de Souza W

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...

journal_title：Biology of the cell

authors： Jablonski EM,Hughes FM Jr

更新日期：2006-01-01 00:00:00

abstract：:Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...

journal_title：Biology of the cell

authors： Desnos C,Huet S,Darchen F

更新日期：2007-08-01 00:00:00

abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...

journal_title：Biology of the cell

authors： Quemener V,Moulinoux JP,Khan NA,Seiler N

更新日期：1990-01-01 00:00:00

abstract：:Since almost two decades, it is known that progesterone is responsible of the release of the prophase I arrest of amphibian oocytes and leads to the activation of the universal MPF, through a puzzling transduction pathway. It involves negative regulation of the cAMP-dependent protein kinase (PKA) and synthesis of new ...

journal_title：Biology of the cell

authors： Karaiskou A,Dupré A,Haccard O,Jessus C

更新日期：2001-09-01 00:00:00

abstract：:Infection with Mycobacterium ulcerans results in a necrotising skin disease known as a Buruli ulcer, the pathology of which is directly linked to the bacterial production of the toxin mycolactone. Recent studies have identified the protein translocation machinery of the endoplasmic reticulum (ER) membrane as the prima...

journal_title：Biology of the cell

authors： Demangel C,High S

更新日期：2018-11-01 00:00:00

abstract：:We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...

journal_title：Biology of the cell

authors： Bernard B,Prétet JL,Charlot JF,Mougin C

更新日期：2003-01-01 00:00:00

abstract：:Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop...

journal_title：Biology of the cell

authors： Kowit JD,Linck RW,Kenney DM

更新日期：1988-01-01 00:00:00

abstract：:ESCRTs (endosomal sorting complexes required for transport) were first discovered in yeast and are known to be required in the biogenesis of the MVB (multivesicular body). Most ESCRT research has been carried out in vitro using models such as yeast and mammalian cells in culture. The role of the ESCRTs genes in endoso...

journal_title：Biology of the cell

authors： Michelet X,Djeddi A,Legouis R

更新日期：2010-01-13 00:00:00

abstract：:LDs (lipid droplets) have long been considered as inert particles used by the cells to store fatty acids and sterols as esterified non-toxic lipid species (i.e. triacylglycerols and steryl esters). However, accumulating evidence suggests that LDs behave as a dynamic compartment, which is involved in the regulation of ...

journal_title：Biology of the cell

authors： Hapala I,Marza E,Ferreira T

更新日期：2011-06-01 00:00:00

abstract：:For the purpose of investigating the nature of the nervous factor which controls cell proliferation in limb blastema of Newts, we have cultured primary mesenchymous cells from limb blastemas of Axolotl. The cultures were carried out in Petri dishes (Primaria, Falcon) with a basal medium with contained diluted MEM supp...

journal_title：Biology of the cell

authors： Albert P,Boilly B

更新日期：1986-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The ribonucleases (RNases) constitute a heterogeneous group of enzymes, which exert diverse and specific biological functions. Several RNases have been shown to control gene expression and cell differentiation. RNASET2, a novel member of the Rh/T2/S family of RNases, exerts micro-environmental co...

journal_title：Biology of the cell

authors： Vidalino L,Monti L,Haase A,Moro A,Acquati F,Taramelli R,Macchi P

更新日期：2012-01-01 00:00:00

abstract：:Background information. In vitro-cultured microspores, after an appropriate stress treatment, can switch towards an embryogenic pathway. This process, known as microspore embryogenesis, is an important tool in plant breeding. Basic studies on this process in economically interesting crops, especially in recalcitrant p...

journal_title：Biology of the cell

authors： Bárány I,González-Melendi P,Fadón B,Mitykó J,Risueño MC,Testillano PS

更新日期：2005-09-01 00:00:00

abstract：BACKGROUND:The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus-encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of se...

journal_title：Biology of the cell

authors： Singha UK,Tripathi A,Smith JT Jr,Quinones L,Saha A,Singha T,Chaudhuri M

更新日期：2020-10-20 00:00:00

abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...

journal_title：Biology of the cell

authors： Espinosa-Cantellano M,Martínez-Palomo A

更新日期：1991-01-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The assembly and maintenance of cilia depend on IFT (intraflagellar transport) mediated by molecular motors and their interplay with IFT proteins. Here, we have analysed the involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia. RESULTS:Electron microscopy revealed tha...

journal_title：Biology of the cell

authors： Sedmak T,Wolfrum U

更新日期：2011-10-01 00:00:00

abstract：:Recent advances in understanding the in vitro translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane have established a molecular basis for the initial reactions predicted by the signal hypothesis. The first two events involve a transient arrest of nascent chain elongation, followed by a ...

journal_title：Biology of the cell

authors： Hortsch M,Meyer DI

更新日期：1984-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...

journal_title：Biology of the cell

authors： Zimowska M,Swierczynska M,Ciemerych MA

更新日期：2013-08-01 00:00:00

abstract：:The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...

journal_title：Biology of the cell

authors： Mattei MG,Duprey P,Li ZL,Mattei JF,Paulin D

更新日期：1989-01-01 00:00:00

abstract：:The membrane content in phospholipids along the secretory pathway in rat pancreatic B-cells was studied in situ by high-resolution cytochemistry, applying the recently introduced phospholipase A2-gold technique. The gold particles were mostly associated with cell membranes, and the various types of membranes were labe...

journal_title：Biology of the cell

authors： Coulombe PA,Bendayan M

更新日期：1989-01-01 00:00:00

abstract：:The communication of calcium signals between cells is known to be operative between neurons where these signals integrate intimately with electrical and chemical signal communication at synapses. Recently, it has become clear that glial cells also exchange calcium signals between each other in cultures and in brain sl...

journal_title：Biology of the cell

authors： Braet K,Cabooter L,Paemeleire K,Leybaert L

更新日期：2004-02-01 00:00:00

abstract：BACKGROUND INFORMATION:Proliferating cell nuclear antigen (PCNA) is a key component of the DNA replication machinery involved in the process of DNA elongation, recombination, methylation and repair. We have used PCNA fused with green fluorescent protein (GFP-PCNA) as a convenient tool to show the progress of S-phase in...

journal_title：Biology of the cell

authors： Kisielewska J,Lu P,Whitaker M

更新日期：2005-03-01 00:00:00

abstract：:Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluo...

journal_title：Biology of the cell

authors： Olins DE,Olins AL,Robert-Nicoud M,Jovin TM,Wehland J,Weber K

更新日期：1989-01-01 00:00:00

abstract：:Our knowledge of cell signalling pathways in the molluscan immune system and their response to immunological challenge is currently poor. The present study focused on the Protein Kinase C (PKC) pathway in the immune cells (haemocytes) of Lymnaea stagnalis and its response following exposure to bacterial lipopolysaccha...

journal_title：Biology of the cell

authors： Walker AJ,Plows LD

更新日期：2003-11-01 00:00:00