Abstract:
BACKGROUND INFORMATION:The ribonucleases (RNases) constitute a heterogeneous group of enzymes, which exert diverse and specific biological functions. Several RNases have been shown to control gene expression and cell differentiation. RNASET2, a novel member of the Rh/T2/S family of RNases, exerts micro-environmental control of malignancy in different experimental models with a general onco-suppressor activity, since it prevents cancer proliferation. Indeed, RNASET2 was found to be downregulated at the transcript level in several primary ovarian tumours or cell lines and in melanoma cell lines. Although recent works shed light on the biological role of RNASET2 in delaying tumour growth, its trafficking within the cell is still poorly understood. RNASET2 seems to play diverse biological roles including turnover of tRNA in yeast as well as rRNA degradation in zebrafish. RESULTS:Here, we have studied the intracellular trafficking of RNASET2 in mammalian cells. RNASET2 co-localizes with markers for the trans-Golgi network (TGN), which is the central sorting and processing station of the secretory pathway. Moreover, using the temperature-sensitive vesicular stomatitis glycoprotein, we demonstrate that RNASET2 undergoes delivery to the plasma membrane. In contrast to other RNA-interacting proteins, RNASET2 does not accumulate in stress granules upon metabolic stress in mammalian cells. Surprisingly, RNASET2 shows co-localization with processing bodies (P-bodies), which increases upon metabolic stress. Finally, cells lacking RNASET2 show a reduced numbers of P-bodies. CONCLUSIONS:In this study, we have identified two distinct cellular pools of RNASET2-containing granules. One pool undergoes membrane delivery using the TGN, and it is released to the extracellular environment. The second pool is recruited into P-bodies, suggesting a possible involvement of RNASET2 in P-body formation in mammalian cells.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Vidalino L,Monti L,Haase A,Moro A,Acquati F,Taramelli R,Macchi Pdoi
10.1111/boc.201100092subject
Has Abstractpub_date
2012-01-01 00:00:00pages
13-21issue
1eissn
0248-4900issn
1768-322Xjournal_volume
104pub_type
杂志文章abstract::The micromeres, the first cells to be specified in sea urchin embryos, are generated by unequal cleavage at the fourth cell division. The micromeres differentiate autonomously to form spicules and dispatch signals to induce endomesoderm in the neighbouring macromeres cells in the embryo. Using a calcium indicator Fura...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/j.biolcel.2003.11.009
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060012
更新日期:2006-10-01 00:00:00
abstract::The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(94)90066-3
更新日期:1994-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(00)01107-2
更新日期:2000-12-01 00:00:00
abstract:BACKGROUND INFORMATION:Human gingival tissues are prone to hyperplasia under inflammatory stimuli. We have identified gingival tissue-specific mesenchymal stem cells (GMSCs) and found their functional change being correlated with drug-induced gingival hyperplasia. However, whether these cells exhibit characteristics of...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200064
更新日期:2013-06-01 00:00:00
abstract::A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(02)00006-0
更新日期:2002-10-01 00:00:00
abstract::Separation by velocity sedimentation at unit gravity according to the STAPUT system of Miller and Phillips was applied to a population of rabbit megakaryocytes previously enriched by density gradient centrifugation. By this means, 80,000 to 100,000 megakaryocytes with 100% purity were collected in eight fractions acco...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00231.x
更新日期:1983-01-01 00:00:00
abstract::Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90016-t
更新日期:1992-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1996.tb00953.x
更新日期:1996-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1998-10-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201900085
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND INFORMATION:Caspases are cysteine proteases that mediate apoptosis (programmed cell death) initiation and execution. Apoptosis is a conserved mechanism shared by all metazoans, although its physiological function and complexity show considerable taxon-dependent variations. To gain insight into the caspase re...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20050018
更新日期:2005-11-01 00:00:00
abstract::Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20090098
更新日期:2010-02-17 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/0248-4900(92)90137-p
更新日期:1992-01-01 00:00:00
abstract::The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the laye...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)84780-0
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:Immunofluorescence microscopy revealed that protein kinase C (PKC)...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201500032
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND INFORMATION:AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino aci...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040091
更新日期:2005-11-01 00:00:00
abstract::Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(88)90072-x
更新日期:1988-01-01 00:00:00
abstract::The aim of a large number of studies on G protein-coupled receptors was centered on understanding the structural basis of their main functional properties. Here, we will briefly review the results obtained on the alpha1-adrenergic receptor subtypes belonging to the rhodopsin-like family of receptors. These findings co...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/j.biolcel.2004.03.009
更新日期:2004-06-01 00:00:00
abstract::We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00557.x
更新日期:1987-01-01 00:00:00
abstract::The centrosome of the slime mould Dictyostelium discoideum displays a morphology markedly different from centriolar centrosomes or yeast spindle pole bodies, while fulfilling the same conserved functions in the organization of the microtubule cytoskeleton. Recent advances suggest that the Dictyostelium centrosome may ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(99)80092-6
更新日期:1999-05-01 00:00:00
abstract::Cilia from quail oviduct were isolated with their membrane. The ultrastructural study revealed a good preservation of cilia in the purified fraction. Electrophoresis on SDS-PAGE showed a reproducible pattern of ciliary proteins, the major bands being those of tubulins 57 kDa and dyneins above 250 kDa. Among the minor ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00487.x
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Carcinoma of the oesophagus is the sixth leading cause of cancer death in the western world and is associated with a 5-year survival of less than 15%. Recent evidence suggests that stromal-epithelial interactions are fundamental in carcinogenesis. The advent of co-culture techniques permits the i...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100071
更新日期:2010-12-01 00:00:00
abstract::In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80017-0
更新日期:1994-01-01 00:00:00
abstract::The major finding of the present study is that the ultrastructural organization of the neuromuscular synapse can be modified by a small, 4-week-long, physiological increase in the locomotor activity of the extensor digitorum longus muscle of normal adult rats trained to walk. This study measures these plastic adaptati...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(99)80078-1
更新日期:1997-03-01 00:00:00
abstract::The oligochaete annelids, belonging to the subfamily Tubificinae, produce two types of spermatozoa: eusperm (the fertilising ones) and parasperm, protecting and carrying the eusperm. The pathway for the production of the two types is common until the onset of meiosis, but then a regular meiosis produces eusperm, where...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(02)01185-1
更新日期:2002-05-01 00:00:00
abstract::A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00431.x
更新日期:1985-01-01 00:00:00
abstract::Gap junctions are widely expressed in the various cell types of the central nervous system. These specialized membrane intercellular junctions provide the morphological support for direct electrical and biochemical communication between adjacent cells. This intercellular coupling is controlled by neurotransmitters and...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(02)00016-3
更新日期:2002-11-01 00:00:00
abstract::beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040531
更新日期:2005-12-01 00:00:00