Megakaryocytes separation in homogeneous classes by unit gravity sedimentation: physico-chemical, ultrastructural and cytophotometric characterizations.

abstract：:The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...

journal_title：Biology of the cell

authors： Hill AM,Cassoly R,Chetrite G,Pantaloni D

更新日期：1985-01-01 00:00:00

abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...

journal_title：Biology of the cell

authors： Denefle JP,Zhu QL,Lechaire JP

更新日期：1989-01-01 00:00:00

abstract：:We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...

journal_title：Biology of the cell

authors： Bernard B,Prétet JL,Charlot JF,Mougin C

更新日期：2003-01-01 00:00:00

abstract：:Acetylcholinesterase activity was detected in the carotid labyrinth of amphibians by both biochemical and histochemical methods. The histochemical tests showed enzyme activity in surfaces of afferent and efferent nerve terminals in contact with type I cells, but none within the type I cells. Acetylcholinesterase activ...

journal_title：Biology of the cell

authors： Poullet M,Verdiere M

更新日期：1985-01-01 00:00:00

abstract：:The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...

journal_title：Biology of the cell

authors： Dambly-Chaudière C,Sapède D,Soubiran F,Decorde K,Gompel N,Ghysen A

更新日期：2003-12-01 00:00:00

abstract：:Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X-ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies. It is now clear that a powerful approach is t...

journal_title：Biology of the cell

authors： Wade RH,Hewat EA

更新日期：1994-01-01 00:00:00

abstract：:The oligochaete annelids, belonging to the subfamily Tubificinae, produce two types of spermatozoa: eusperm (the fertilising ones) and parasperm, protecting and carrying the eusperm. The pathway for the production of the two types is common until the onset of meiosis, but then a regular meiosis produces eusperm, where...

journal_title：Biology of the cell

authors： Ferraguti M,Fascio U,Boi S

更新日期：2002-05-01 00:00:00

abstract：:We studied the behaviour in interphase and mitotic human cells of a 125 kDa (pI 6.5) antigen, associated with the nuclear matrix and detected in proliferating cells. Indirect immunofluorescence with a specific monoclonal antibody reveals that during interphase in WISH and Namalwa cells, as well as phytohaemagglutinin-...

journal_title：Biology of the cell

authors： Todorov IT,Philipova PN,Zhelev NZ,Hadjiolov AA

更新日期：1988-01-01 00:00:00

abstract：:Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...

journal_title：Biology of the cell

authors： Migné C,Prensier G,Cornu A,Grenet E

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...

journal_title：Biology of the cell

authors： Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

更新日期：2008-03-01 00:00:00

abstract：:We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10(-8) M ouabain, the number of domes seen on Caco-2 cell monolayers grown on plastic dishes wa...

journal_title：Biology of the cell

authors： Ramond MJ,Martinot-Peignoux M,Erlinger S

更新日期：1985-01-01 00:00:00

abstract：:Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady-state fluorescence anisotropy and fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemog...

journal_title：Biology of the cell

authors： Kantar A,Giorgi PL,Curatola G,Fiorini R

更新日期：1992-01-01 00:00:00

abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

journal_title：Biology of the cell

authors： Rebelo L,Carmo-Fonseca M,Moura TF

更新日期：1992-01-01 00:00:00

abstract：:We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...

journal_title：Biology of the cell

authors： Whittembury G,González E,Gutiérrez AM,Echevarría M,Hernández CS

更新日期：1997-08-01 00:00:00

abstract：:Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...

journal_title：Biology of the cell

authors： La Carbona S,Le Goff C,Le Goff X

更新日期：2006-11-01 00:00:00

abstract：:Bistramide A, a new toxin isolated from a New Caledonian Urochordata, shows an antiproliferative effect on a non-small-cell lung carcinoma line in vitro and G1-blockade. In this work, the growth arrest induced by bistramide A was shown to be irreversible as assessed by growth kinetics of pretreated cells. Furthermore,...

journal_title：Biology of the cell

authors： Riou D,Roussakis C,Robillard N,Biard JF,Verbist JF

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...

journal_title：Biology of the cell

authors： Buschke DG,Squirrell JM,Fong JJ,Eliceiri KW,Ogle BM

更新日期：2012-06-01 00:00:00

abstract：:A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rathe...

journal_title：Biology of the cell

authors： Amos WB,White JG

更新日期：2003-09-01 00:00:00

abstract：BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...

journal_title：Biology of the cell

authors： Golpour M,Fattahi S,Niaki HA,Hadipoor A,Abedian Z,Ahangarian GR,Parsian H,Mosapour A,Khorasani HR,Vaziri HR,Bijani A,Mostafazadeh A

更新日期：2014-05-01 00:00:00

abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...

journal_title：Biology of the cell

authors： Tronchin G,Bouchara JP,Larcher G,Lissitzky JC,Chabasse D

更新日期：1993-01-01 00:00:00

abstract：:We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...

journal_title：Biology of the cell

authors： Le Bivic A,Hirn M,Reggio H

更新日期：1987-01-01 00:00:00

abstract：:Alpha 2-macroglobulin (alpha 2M) is a plasma inhibitor of proteinases, the steric mechanism of which is based on a considerable conformational change. The typical and distinct H-like shape of alpha 2M-chymotrypsin (alpha 2M-chy) complexes seen by electron microscopy led us to an ultrastructural study of the binding of...

journal_title：Biology of the cell

authors： Boisset N,Taveau JC,Barray M,Van Leuven F,Delain E,Lamy JN

更新日期：1988-01-01 00:00:00

abstract：:A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...

journal_title：Biology of the cell

authors： Hanssen E,Franc S,Garrone R

更新日期：1998-06-01 00:00:00

abstract：:Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...

journal_title：Biology of the cell

authors： Maronde E,Schomerus C,Stehle JH,Korf HW

更新日期：1997-11-01 00:00:00

abstract：:Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....

journal_title：Biology of the cell

authors： Benali R,Dupuit F,Chevillard M,Jacquot J,Haye B,Puchelle E

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The ribonucleases (RNases) constitute a heterogeneous group of enzymes, which exert diverse and specific biological functions. Several RNases have been shown to control gene expression and cell differentiation. RNASET2, a novel member of the Rh/T2/S family of RNases, exerts micro-environmental co...

journal_title：Biology of the cell

authors： Vidalino L,Monti L,Haase A,Moro A,Acquati F,Taramelli R,Macchi P

更新日期：2012-01-01 00:00:00

abstract：:Regulated exocytosis of defensive secretory organelles, the trichocysts, as well as a transient Ca(2+)-influx can be induced in Paramecium by aminoethyldextran (Kerboeuf and Cohen, J Cell Biol (1990) 111, 2527). Knoll et al (Febs Lett (1992) 304, 265) reported that veratridine was also a secretagogue for Paramecium. H...

journal_title：Biology of the cell

authors： Kerboeuf D,Cohen J

更新日期：1996-01-01 00:00:00

abstract：:Changes in the organization of centrosomes in mouse splenic T lymphocytes stimulated by concanavalin A (con A) were examined by electron microscopy of serial sections. In both resting and stimulated lymphocytes the single centrosome consists of a pair of centrioles, satellite bodies, and pericentriolar material. In re...

journal_title：Biology of the cell

authors： Schweitzer I,Brown DL

更新日期：1984-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...

journal_title：Biology of the cell

authors： Calzia D,Barabino S,Bianchini P,Garbarino G,Oneto M,Caicci F,Diaspro A,Tacchetti C,Manni L,Candiani S,Ravera S,Morelli A,Enrico Traverso C,Panfoli I

更新日期：2013-08-01 00:00:00

abstract：:Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...

journal_title：Biology of the cell

authors： Le Guellec D

更新日期：1998-07-01 00:00:00