Abstract:
:Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputter-coated with silver, were found to be free of focal aluminum surface contamination and thus minimised substrate induced artefacts in the tissue aluminium ion image. SIMS imaging of aluminium secondary ions at a mass resolution that eliminated the major molecular interferences, combined with a photomontage technique provided a unique strategy for studying aluminium distribution in tissue unrivalled by other spatially resolved microanalytical techniques such as laser microprobe mass spectrometry or X-ray microanalysis. Using this strategy, high densities of focal aluminium accumulations have been demonstrated in the cerebral cortex of the majority of chronic renal dialysis patients studied. In contrast, such aluminium accumulations were absent in control patients. SIMS imaging of aluminium appeared to provide much better discrimination between the dialysis patient group and the control group than one of the most widely used techniques for measuring aluminium in bulk samples, graphite furnace atomic absorption spectrometry. Preliminary studies have shown the feasibility of quantifying focal aluminium SIMS images obtained from brain tissue using aluminium-loaded brain homogenates as reference standards.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Candy JM,Oakley AE,Mountfort SA,Taylor GA,Morris CM,Bishop HE,Edwardson JAdoi
10.1016/0248-4900(92)90016-tsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
109-18issue
1eissn
0248-4900issn
1768-322Xjournal_volume
74pub_type
杂志文章abstract:BACKGROUND INFORMATION:The mitogen-inducible gene-6 (Mig-6) is a non-kinase scaffolding adaptor protein. It has been shown that Mig-6 may play important roles in regulating stress response, maintaining homeostasis and functioning as a tumour suppressor. In this study, we investigated the role of Mig-6 in cellular senes...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200052
更新日期:2013-10-01 00:00:00
abstract::The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1989.tb00867.x
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The role of the jelly coat that surrounds the amphibian oocytes has been widely discussed, but is poorly understood. The presence of the jelly coat is essential for fertilization. However, the structure and function of the molecules that comprise the jelly coat have not been thoroughly documented...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20050051
更新日期:2006-07-01 00:00:00
abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90343-2
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Caspases are cysteine proteases that mediate apoptosis (programmed cell death) initiation and execution. Apoptosis is a conserved mechanism shared by all metazoans, although its physiological function and complexity show considerable taxon-dependent variations. To gain insight into the caspase re...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20050018
更新日期:2005-11-01 00:00:00
abstract::Previous studies suggest that oligodeoxynucleotide (ODN) cellular uptake is cell cycle-dependent which may have important implications in cancer cell targeting. To further our understanding of ODN transport and activity, this study examines the relationships between the cell cycle, ODN cellular uptake, intracellular t...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Co-isolated residual nuclear shells and residual nucleoli from membrane-depleted rat liver nuclei were spread according to Kleinschmidt's method. Comparison of the spread residual structures isolated from nuclear shells and spread pore complex-lamina isolated from nuclear envelopes showed that these residual structure...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00438.x
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The endometrial epithelial cell membrane is a key interface in female reproductive biology. Steroid hormones play a predominant role in cyclic changes which occur at this interface during the female menstrual cycle. Specific changes in the morphology of the endometrial epithelial cell surface bec...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080189
更新日期:2009-06-04 00:00:00
abstract::Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00274.x
更新日期:1984-01-01 00:00:00
abstract::This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::A new class of fluorescent dye which upon excitation at 488 nm turns red is shown to be probe-suitable for using in flow cytometry alone or in conjunction with fluorescein derivatives. 7-dimethylamino 3-(p-formylstyryl) 1,4 benzoxazin 2-one is suitable for rendering microorganisms, such as Plasmodium merozoites and ce...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the tw...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00523.x
更新日期:1987-01-01 00:00:00
abstract::A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00431.x
更新日期:1985-01-01 00:00:00
abstract::Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90140-v
更新日期:1992-01-01 00:00:00
abstract::A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rathe...
journal_title:Biology of the cell
pub_type: 历史文章,杂志文章,评审
doi:10.1016/s0248-4900(03)00078-9
更新日期:2003-09-01 00:00:00
abstract::The water permeability of collecting ducts is greatly increased by the antidiuretic hormone, vasopressin (VP). Freeze-fracture studies were carried out to test if this permeability increase is associated with the appearance of intramembrane particle (IMP) aggregates and whether increased doses of VP lead to an increas...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20050058
更新日期:2006-03-01 00:00:00
abstract::This review highlights new developments in miRNA as diagnostic and surveillance tools in diseases damaging the renal proximal tubule mediated by endothelin in the field of renal carcinoma, proteinuric kidney disease and tubulotoxicity. A new mechanism in the miRNA regulation of proteins leads to the binding of the miR...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/boc.201800059
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300003
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND INFORMATION:Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nuc...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080054
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND INFORMATION:Available data concerning the sperm morphology of teleost fishes demonstrate wide variation. In the present study, the spermatozoa of Siberian sturgeon (Acipenser baerii Brandt, 1869), a chondrostean fish, was investigated. In contrast with teleost fish, chondrostean spermatozoa have a head with ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060060
更新日期:2007-02-01 00:00:00
abstract::Although much of eukaryotic cell biology is highly conserved, the most biomedically relevant organisms are often the most difficult or expensive to study. Non-vertebrate model organisms offer a practical and accessible alternative. Large collections of loss-of-function mutants have now been generated in the nematode C...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070005
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND INFORMATION:Homotypic internalisation of tumour cells has frequently been observed in tumour tissue sections. Events of non-professional phagocytosis, however, may also occur in normal tissue if the number of dying cells exceeds the phagocytic capacity of professional phagocytes such as macrophages and dendr...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201400090
更新日期:2015-10-01 00:00:00
abstract::Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage-like differentiation i.e. the pres...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00309.x
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND INFORMATION:In eukaryotic cells, autophagy is considered a lysosomal catabolic process which participates in the degradation of intracellular components in a vacuolar structure termed autolysosome. This pathway plays a significant role in the erythropoiesis process, contributing to the clearance of some orga...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201500058
更新日期:2016-04-01 00:00:00
abstract::In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80017-0
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100112
更新日期:2011-11-01 00:00:00
abstract::Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)89429-9
更新日期:1995-01-01 00:00:00
abstract::The isolation, characterization, and expression of a novel cDNA encoding a Trypanosoma cruzi polypeptide (TcAc2), homologous to various small stress proteins and glutathione S-transferases, are described. The deduced amino-acid sequence revealed two domains sharing 27% identity and an additional 27% similarity to each...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(94)90011-6
更新日期:1994-01-01 00:00:00
abstract::The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90037-2
更新日期:1992-01-01 00:00:00