Internalization and recycling of lutropin receptors upon stimulation by porcine lutropin and human choriogonadotropin in porcine Leydig cells.

Abstract:

:Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the two hormones at 35 degrees C. Both hormones produced a rapid dose-dependent decrease in the total number of the gonadotropin receptors present on the cell surface with a half-life of 8-10 min. This decrease was reversible upon hormone removal and receptors were recovered on the cell surface with the same half-life of 8-10 min. With pLH, the receptors were recycled in a free state, but in the presence of hCG the receptors were recycled in an occupied state. This difference could be related to the higher affinity of hCG for the receptor in 150 mM NaCl buffer (Ka = 1.6 X 10(9) for hCG and 1.5 X 10(7) for pLH) and higher stability to acid pH of the hCG-receptor complex (dissociation pK = 3.7 for hCG and 4.5 for pLH).

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Genty N,Salesse R,Garnier J

doi

10.1111/j.1768-322x.1987.tb00523.x

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

129-35

issue

2

eissn

0248-4900

issn

1768-322X

journal_volume

59

pub_type

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