How the confocal laser scanning microscope entered biological research.

Abstract:

:A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rather than vice-versa and a high magnification at the level of the detector, allowing the use of a macroscopic iris. These were followed by an achromatic all-reflective relay system, a non-confocal transmission detector and novel software for control and basic image processing. This design was commercialized successfully and has been produced and developed over 17 years, surviving challenges from alternative technologies, including solid-state scanning systems. Lessons are pointed out from the unusual nature of the original funding and research environment. Attention is drawn to the slow adoption of the instrument in diagnostic medicine, despite promising applications.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Amos WB,White JG

doi

10.1016/s0248-4900(03)00078-9

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

335-42

issue

6

eissn

0248-4900

issn

1768-322X

pii

S0248490003000789

journal_volume

95

pub_type

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