Benzoxazinone derivatives: new fluorescent probes for two-color flow cytometry analysis using one excitation wavelength.

abstract：:beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...

journal_title：Biology of the cell

authors： Gavard J,Mège RM

更新日期：2005-12-01 00:00:00

abstract：:Genome replication and assembly of viruses often takes place in specific intracellular compartments where viral components concentrate, thereby increasing the efficiency of the processes. For a number of viruses the formation of 'factories' has been described, which consist of perinuclear or cytoplasmic foci that most...

journal_title：Biology of the cell

authors： Novoa RR,Calderita G,Arranz R,Fontana J,Granzow H,Risco C

更新日期：2005-02-01 00:00:00

abstract：:Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that are released from HCV polyprotein by signal peptidase cleavage. These proteins assemble as a noncovalent heterodimer that is retained in the endoplasmic reticulum. The transmembrane domains of E1 and E2 are multifunctional and play a major rol...

journal_title：Biology of the cell

authors： Voisset C,Dubuisson J

更新日期：2004-08-01 00:00:00

abstract：:Previous experiments have revealed a relatively weak electrostatic binding capacity of in vitro reconstituted intermediate filaments (IFs) as well as of natural IFs of whole cell mount preparations for purified ribosomal particles of mammalian origin. In order to demonstrate that such associations also occur in vivo, ...

journal_title：Biology of the cell

authors： Traub P,Bauer C,Hartig R,Grüb S,Stahl J

更新日期：1998-07-01 00:00:00

abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...

journal_title：Biology of the cell

authors： Espinosa-Cantellano M,Martínez-Palomo A

更新日期：1991-01-01 00:00:00

abstract：:The diversity of microtubular networks was analyzed in quail oviduct and in Paramecium cells using conventional and confocal immunofluorescence as well as pre- and post-embedding EM immunocytochemistry with a variety of anti-tubulin antibodies. The 6-11B-1 monoclonal antibody, specific for the post-translational acety...

journal_title：Biology of the cell

authors： Adoutte A,Delgado P,Fleury A,Levilliers N,Lainé MC,Marty MC,Boisvieux-Ulrich E,Sandoz D

更新日期：1991-01-01 00:00:00

abstract：:Gap junctions are widely expressed in the various cell types of the central nervous system. These specialized membrane intercellular junctions provide the morphological support for direct electrical and biochemical communication between adjacent cells. This intercellular coupling is controlled by neurotransmitters and...

journal_title：Biology of the cell

authors： Rouach N,Avignone E,Même W,Koulakoff A,Venance L,Blomstrand F,Giaume C

更新日期：2002-11-01 00:00:00

abstract：:Cells sense and respond to the biochemical and physical properties of the extracellular matrix (ECM) through adhesive structures that bridge the cell cytoskeleton and the surrounding environment. Integrin-mediated adhesions interact with specific ECM proteins and sense the rigidity of the substrate to trigger signalli...

journal_title：Biology of the cell

authors： Seetharaman S,Etienne-Manneville S

更新日期：2018-03-01 00:00:00

abstract：:A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...

journal_title：Biology of the cell

authors： Eggena P,Ma CL,Fahrenholz F,Buku A,Schwartz IL

更新日期：1985-01-01 00:00:00

abstract：BACKGROUND INFORMATION:AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino aci...

journal_title：Biology of the cell

authors： Li H,Kamiie J,Morishita Y,Yoshida Y,Yaoita E,Ishibashi K,Yamamoto T

更新日期：2005-11-01 00:00:00

abstract：BACKGROUND INFORMATION:At fertilization in mammalian eggs, the sperm induces a series of Ca(2+) oscillations via the production of inositol 1,4,5-trisphosphate. Increased inositol 1,4,5-trisphosphate production appears to be triggered by a sperm-derived PLCzeta (phospholipase C-zeta) that enters the egg after gamete fu...

journal_title：Biology of the cell

authors： Yu Y,Halet G,Lai FA,Swann K

更新日期：2008-11-01 00:00:00

abstract：:Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due ...

journal_title：Biology of the cell

authors： Roingeard P,Hourioux C,Blanchard E,Brand D,Ait-Goughoulte M

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND INFORMATION:Autofluorescence spectroscopy is a powerful tool for molecular histology and for following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, it is mostly limited to visible and near infrared excitation of the samples. Several interestin...

journal_title：Biology of the cell

authors： Jamme F,Kascakova S,Villette S,Allouche F,Pallu S,Rouam V,Réfrégiers M

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...

journal_title：Biology of the cell

authors： Silva AP,Kaufmann JE,Vivancos C,Fakan S,Cavadas C,Shaw P,Brunner HR,Vischer U,Grouzmann E

更新日期：2005-06-01 00:00:00

abstract：BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...

journal_title：Biology of the cell

authors： Zimowska M,Swierczynska M,Ciemerych MA

更新日期：2013-08-01 00:00:00

abstract：:Co-isolated residual nuclear shells and residual nucleoli from membrane-depleted rat liver nuclei were spread according to Kleinschmidt's method. Comparison of the spread residual structures isolated from nuclear shells and spread pore complex-lamina isolated from nuclear envelopes showed that these residual structure...

journal_title：Biology of the cell

authors： Bureau J,Hubert J,Bouteille M

更新日期：1986-01-01 00:00:00

abstract：:Although much of eukaryotic cell biology is highly conserved, the most biomedically relevant organisms are often the most difficult or expensive to study. Non-vertebrate model organisms offer a practical and accessible alternative. Large collections of loss-of-function mutants have now been generated in the nematode C...

journal_title：Biology of the cell

authors： Haag ES

更新日期：2007-06-01 00:00:00

abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

journal_title：Biology of the cell

authors： Rebelo L,Carmo-Fonseca M,Moura TF

更新日期：1992-01-01 00:00:00

abstract：:Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...

journal_title：Biology of the cell

authors： Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

更新日期：1988-01-01 00:00:00

abstract：:Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stag...

journal_title：Biology of the cell

authors： Jessus C,Huchon D,Ozon R

更新日期：1986-01-01 00:00:00

abstract：:This review highlights new developments in miRNA as diagnostic and surveillance tools in diseases damaging the renal proximal tubule mediated by endothelin in the field of renal carcinoma, proteinuric kidney disease and tubulotoxicity. A new mechanism in the miRNA regulation of proteins leads to the binding of the miR...

journal_title：Biology of the cell

authors： Fries JWU

更新日期：2019-07-01 00:00:00

abstract：:Cilia from quail oviduct were isolated with their membrane. The ultrastructural study revealed a good preservation of cilia in the purified fraction. Electrophoresis on SDS-PAGE showed a reproducible pattern of ciliary proteins, the major bands being those of tubulins 57 kDa and dyneins above 250 kDa. Among the minor ...

journal_title：Biology of the cell

authors： Chailley B,Bork K,Gounon P,Sandoz D

更新日期：1986-01-01 00:00:00

abstract：:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...

journal_title：Biology of the cell

authors： Chau MF,Orias E

更新日期：1996-01-01 00:00:00

abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...

journal_title：Biology of the cell

authors： Quemener V,Moulinoux JP,Khan NA,Seiler N

更新日期：1990-01-01 00:00:00

abstract：:Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...

journal_title：Biology of the cell

authors： Guissi-Kadri S,Dhainaut-Courtois N,Porchet M,Curgy JJ

更新日期：1991-01-01 00:00:00

abstract：:Circulating glucocorticoid (GC) levels are thought to modulate the basal activity of pro-opiomelanocortin (POMC) neurons within the mediobasal hypothalamus (MBH) of the male rat. In a recent study we demonstrated that Fos-immunoreactivity (Fos-IR) was spontaneously induced throughout the dark phase of the light/dark c...

journal_title：Biology of the cell

authors： Jamali KA,Tramu G

更新日期：1997-12-01 00:00:00

abstract：:When mouse peritoneal macrophages were made to adhere firmly on glass surface and then removed by sequential treatment with hypotonic triethanolamine and Nonidet P-40, a set of proteins were found to be left behind at the sites of adherent cells. Such glass-adherent proteins were detected as round or ellipsoidal patch...

journal_title：Biology of the cell

authors： Tomita M,Ishikawa H

更新日期：1992-01-01 00:00:00

abstract：:HT 29-D4 is a clonal cell line, derived from the human colon adenocarcinoma cell line HT 29, which can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (Fantini et al. [1986], J. Cell Sci. 83, 235-249). Both undifferentiated and differentiated HT 29-D4 ce...

journal_title：Biology of the cell

authors： Fantini J,Verrier B,Marvaldi J,Mauchamp J

更新日期：1989-01-01 00:00:00

abstract：:The aim of a large number of studies on G protein-coupled receptors was centered on understanding the structural basis of their main functional properties. Here, we will briefly review the results obtained on the alpha1-adrenergic receptor subtypes belonging to the rhodopsin-like family of receptors. These findings co...

journal_title：Biology of the cell

authors： Cotecchia S,Stanasila L,Diviani D,Björklöf K,Rossier O,Fanelli F

更新日期：2004-06-01 00:00:00

abstract：:We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...

journal_title：Biology of the cell

authors： Bernard B,Prétet JL,Charlot JF,Mougin C

更新日期：2003-01-01 00:00:00