Neuropeptide Y expression, localization and cellular transducing effects in HUVEC.

abstract：BACKGROUND INFORMATION:Proliferating cell nuclear antigen (PCNA) is a key component of the DNA replication machinery involved in the process of DNA elongation, recombination, methylation and repair. We have used PCNA fused with green fluorescent protein (GFP-PCNA) as a convenient tool to show the progress of S-phase in...

journal_title：Biology of the cell

authors： Kisielewska J,Lu P,Whitaker M

更新日期：2005-03-01 00:00:00

abstract：:Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...

journal_title：Biology of the cell

authors： Arbona M,Cuenca JB,de Frutos R

更新日期：1992-01-01 00:00:00

abstract：:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...

journal_title：Biology of the cell

authors： Chau MF,Orias E

更新日期：1996-01-01 00:00:00

abstract：:Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due ...

journal_title：Biology of the cell

authors： Roingeard P,Hourioux C,Blanchard E,Brand D,Ait-Goughoulte M

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND INFORMATION:The ribonucleases (RNases) constitute a heterogeneous group of enzymes, which exert diverse and specific biological functions. Several RNases have been shown to control gene expression and cell differentiation. RNASET2, a novel member of the Rh/T2/S family of RNases, exerts micro-environmental co...

journal_title：Biology of the cell

authors： Vidalino L,Monti L,Haase A,Moro A,Acquati F,Taramelli R,Macchi P

更新日期：2012-01-01 00:00:00

abstract：:Photoperiodic control of several biological rhythms is exerted through the inhibitory effect of light on melatonin synthesis in the pineal organ. Hydroxyindole-O-methyltransferase (HIO-MT), the last acting-enzyme in melatonin biosynthesis, constitutes a specific marker of melatoninergic cells. In the present study, an...

journal_title：Biology of the cell

authors： Guerlotte J,Voisin P,Brisson P,Faure JP,Collin JP

更新日期：1988-01-01 00:00:00

abstract：:Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop...

journal_title：Biology of the cell

authors： Kowit JD,Linck RW,Kenney DM

更新日期：1988-01-01 00:00:00

abstract：:SaOS-2 cell line presents osteoblastic characteristics which can be modulated by specific agonists involving also phosphoinositide breakdown. In order to determine whether SaOS-2 cells display a phosphoinositide signalling system not only at the cytosol-cell membrane level but also, as recently reported for other cell...

journal_title：Biology of the cell

authors： Maraldi NM,Zini N,Santi S,Bavelloni A,Valmori A,Marmiroli S,Ognibene A

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...

journal_title：Biology of the cell

authors： Pirger Z,Rácz B,Kiss T

更新日期：2009-02-01 00:00:00

abstract：:Since almost two decades, it is known that progesterone is responsible of the release of the prophase I arrest of amphibian oocytes and leads to the activation of the universal MPF, through a puzzling transduction pathway. It involves negative regulation of the cAMP-dependent protein kinase (PKA) and synthesis of new ...

journal_title：Biology of the cell

authors： Karaiskou A,Dupré A,Haccard O,Jessus C

更新日期：2001-09-01 00:00:00

abstract：:Our recent data suggest that during auto- and allograft recognition in sponges (Porifera), cytokines are differentially expressed. Since the mitogen-activated protein kinase (MAPK) signal transduction modulates the synthesis and release of cytokines, we intended to identify one key molecule of this pathway. Therefore,...

journal_title：Biology of the cell

authors： Böhm M,Schröder HC,Müller IM,Müller WE,Gamulin V

更新日期：2000-04-01 00:00:00

abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

journal_title：Biology of the cell

authors： Rebelo L,Carmo-Fonseca M,Moura TF

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...

journal_title：Biology of the cell

authors： Li Y,Manaligod JM,Weeks DL

更新日期：2010-02-17 00:00:00

abstract：BACKGROUND INFORMATION:In a previous study, we demonstrated that human neutrophils can develop membrane tubulovesicular extensions (TVEs) that are 160-250 nm in width and several micrometres long. These extensions, or cytonemes, are capable of establishing long-range contacts with other cells or bacteria. Cytonemes con...

journal_title：Biology of the cell

authors： Galkina SI,Fedorova NV,Serebryakova MV,Arifulin EA,Stadnichuk VI,Gaponova TV,Baratova LA,Sud'ina GF

更新日期：2015-05-01 00:00:00

abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...

journal_title：Biology of the cell

authors： Palluault F,Dei-Cas E,Slomianny C,Soulez B,Camus D

更新日期：1990-01-01 00:00:00

abstract：:Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of...

journal_title：Biology of the cell

authors： Noaillac-Depeyre J

更新日期：1985-01-01 00:00:00

abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...

journal_title：Biology of the cell

authors： N'Da E,Bonnanfant-Jais ML,Moreau N,Angelier N

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...

journal_title：Biology of the cell

authors： Zhang J,Duan X,Zhang H,Deng Z,Zhou Z,Wen N,Smith AJ,Zhao W,Jin Y

更新日期：2006-10-01 00:00:00

abstract：:The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...

journal_title：Biology of the cell

authors： Charbonneau M,Grandin N

更新日期：1992-01-01 00:00:00

abstract：:This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...

journal_title：Biology of the cell

authors： Dirksen ER

更新日期：1991-01-01 00:00:00

abstract：:ERM (ezrin/radixin/moesin) proteins, concentrated in actin rich cell-surface structures, cross-link actin filaments with the plasma membrane. They are involved in the formation of microvilli, cell-cell adhesion, maintenance of cell shape, cell motility and membrane trafficking. Recent analyses reveal that they are not...

journal_title：Biology of the cell

authors： Louvet-Vallée S

更新日期：2000-08-01 00:00:00

abstract：:As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...

journal_title：Biology of the cell

authors： Crabbe J,Beaujean V,Devuyst O

更新日期：1989-01-01 00:00:00

abstract：:HT 29-D4 is a clonal cell line, derived from the human colon adenocarcinoma cell line HT 29, which can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (Fantini et al. [1986], J. Cell Sci. 83, 235-249). Both undifferentiated and differentiated HT 29-D4 ce...

journal_title：Biology of the cell

authors： Fantini J,Verrier B,Marvaldi J,Mauchamp J

更新日期：1989-01-01 00:00:00

abstract：:Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the tw...

journal_title：Biology of the cell

authors： Genty N,Salesse R,Garnier J

更新日期：1987-01-01 00:00:00

abstract：:We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10(-8) M ouabain, the number of domes seen on Caco-2 cell monolayers grown on plastic dishes wa...

journal_title：Biology of the cell

authors： Ramond MJ,Martinot-Peignoux M,Erlinger S

更新日期：1985-01-01 00:00:00

abstract：:The water permeability of collecting ducts is greatly increased by the antidiuretic hormone, vasopressin (VP). Freeze-fracture studies were carried out to test if this permeability increase is associated with the appearance of intramembrane particle (IMP) aggregates and whether increased doses of VP lead to an increas...

journal_title：Biology of the cell

authors： Kubat B,Lorenzen M,Reale E

更新日期：1989-01-01 00:00:00

abstract：:The interaction of cells with extracellular matrix components contributes to their specific differentiation. We studied hepatic peroxisomes and their changing features during embryonic development, and we immunolocalized in the same tissue the extracellular matrix components laminin and collagen IV as well as the inte...

journal_title：Biology of the cell

authors： Depreter M,Nardacci R,Tytgat T,Espeel M,Stefanini S,Roels F

更新日期：1998-12-01 00:00:00

abstract：:Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...

journal_title：Biology of the cell

authors： La Carbona S,Le Goff C,Le Goff X

更新日期：2006-11-01 00:00:00

abstract：BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...

journal_title：Biology of the cell

authors： Clément CMH,Deffieu MS,Dorobantu CM,Baumert TF,Ayala-Nunez NV,Mély Y,Ronde P,Gaudin R

更新日期：2020-05-01 00:00:00

abstract：:A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...

journal_title：Biology of the cell

authors： Hanssen E,Franc S,Garrone R

更新日期：1998-06-01 00:00:00