Molecular heterogeneity of the isolated surface glycoprotein from variant AnTat 1.1 of Trypanosoma brucei brucei.

Abstract:

:Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D-mannopyranoside, beta-mercaptoethanol, and sodium dodecyl sulfate. These VSG fractions showed heterogeneous behaviour on reverse-phase high performance liquid chromatography. The 3 VSG fractions as well as the myristylated VSG of AnTat 1.1 essentially consist of dimer VSG forms linked through a disulfide bridge, as judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis, under reducing and nonreducing conditions.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

doi

10.1016/0248-4900(88)90072-x

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

131-5

issue

2

eissn

0248-4900

issn

1768-322X

pii

0248-4900(88)90072-X

journal_volume

64

pub_type

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