Abstract:
:The water permeability of collecting ducts is greatly increased by the antidiuretic hormone, vasopressin (VP). Freeze-fracture studies were carried out to test if this permeability increase is associated with the appearance of intramembrane particle (IMP) aggregates and whether increased doses of VP lead to an increase in the number and size of particle aggregates in the luminal membrane of principal cells in the isolated cortical collecting duct. Unstimulated cells expressed 17 +/- 6.5 particle aggregates per 100 microns 2. Stimulation with VP at concentrations of 20 or 200 microU/ml increased the number of particle aggregates significantly to 129 +/- 15.8 and 324 +/- 45.8, respectively. The size of the particle aggregates increased from 0.0012 microns 2 under control conditions to 0.025 microns 2 at 20 microU/ml VP and to 0.063 microns 2 at 200 microU/ml VP. In addition, the total area occupied by the IMP increased from 0.02 microns 2/100 microns 2 (controls) to 3.17% and 20.38% (after 20 and 200 microU ADH/ml, respectively). Particle aggregates were also observed in the luminal plasma membrane of isolated collecting ducts fixed immediately after dissection, resembling the in vivo status. These results demonstrate that a dose-dependent relationship exists between the concentration of the applied VP and the number of particle aggregates, as well as the size of the aggregates. Cytoplasmic tubular vesicles in fusion with the apical membrane were observed.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Kubat B,Lorenzen M,Reale Esubject
Has Abstractpub_date
1989-01-01 00:00:00pages
59-63issue
1-2eissn
0248-4900issn
1768-322Xjournal_volume
66pub_type
杂志文章abstract::Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady-state fluorescence anisotropy and fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemog...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90133-l
更新日期:1992-01-01 00:00:00
abstract::The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the laye...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)84780-0
更新日期:1996-01-01 00:00:00
abstract::Cilia from quail oviduct were isolated with their membrane. The ultrastructural study revealed a good preservation of cilia in the purified fraction. Electrophoresis on SDS-PAGE showed a reproducible pattern of ciliary proteins, the major bands being those of tubulins 57 kDa and dyneins above 250 kDa. Among the minor ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00487.x
更新日期:1986-01-01 00:00:00
abstract::In spite of the constant development concerning physical mapping of eukaryotic genomes, the mouse chromosome 19 remains poorly characterized. In order to improve the possibilities for studying this chromosome, we have constructed a chromosome-specific EcoRI DNA fragment library from mouse chromosomes 19 sorted by flow...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90322-t
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Human gingival tissues are prone to hyperplasia under inflammatory stimuli. We have identified gingival tissue-specific mesenchymal stem cells (GMSCs) and found their functional change being correlated with drug-induced gingival hyperplasia. However, whether these cells exhibit characteristics of...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200064
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201600050
更新日期:2017-06-01 00:00:00
abstract::The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1989.tb00867.x
更新日期:1989-01-01 00:00:00
abstract::Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20050058
更新日期:2006-03-01 00:00:00
abstract::Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00574.x
更新日期:1987-01-01 00:00:00
abstract::A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(02)00006-0
更新日期:2002-10-01 00:00:00
abstract::Netrins are secreted proteins that play a crucial role in neuronal migration and in axon guidance during the development of the nervous system. Netrin-1 has been shown to interact with the transmembrane receptors DCC and UNC5H and these receptors appeared of key importance in mediating the chemotropic activity of netr...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(03)00072-8
更新日期:2003-10-01 00:00:00
abstract:BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20090098
更新日期:2010-02-17 00:00:00
abstract::The ultrastructure of the Drosophila subobscura chromosome regions around the breakpoints of the complex E1 + 2 + 9 + 12 gene arrangement was analyzed. This overlapping inversion is formed by the association of the E1, E2, E9, and E12 simple inversions. Ultrastructure of sections involving 58D/59A, 61C/D, 62D/63A, 64B...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90359-b
更新日期:1990-01-01 00:00:00
abstract::The interaction of cells with extracellular matrix components contributes to their specific differentiation. We studied hepatic peroxisomes and their changing features during embryonic development, and we immunolocalized in the same tissue the extracellular matrix components laminin and collagen IV as well as the inte...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Effect of estradiol-17 beta on rat vaginal epithelial cells (VEC) was studied by transmission electron microscopy. During the normal estrous cycle in adult rats keratinization and exfoliation of VEC was observed only in the estrus phase, though tonofilament bundles were seen distributed in the cytoplasm of the VEC dur...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300063
更新日期:2014-05-01 00:00:00
abstract::Interaction of sperm and egg at fertilization induces well-coordinated molecular events including specific recognition between species, adhesion and fusion, that lead to the formation of a zygote, a totipotent cell that develops into a new individual. A calcium signal, common to a great number of species, from marine ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(00)01065-0
更新日期:2000-07-01 00:00:00
abstract::Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1990.tb00007.x
更新日期:1990-01-01 00:00:00
abstract::A quantitative ultrastructural study was performed on 56 ejaculates showing anomalies of the sperm axonemal complex. The anomalies comprised either the absence of one, or more often several, axonemal structures, or defective elongation of the doublets. Several characteristics relating to the extent and superimposition...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00253.x
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND INFORMATION:αvβ5 integrin and Mer tyrosine kinase (MerTK) receptors reside at the apical surface of the retinal pigment epithelium (RPE) in the eye to promote the diurnal, synchronised phagocytosis of shed photoreceptor outer segment fragments (POS) that is critical for vision. Phagocytosis assays studying R...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100076
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040131
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The Wiskott-Aldrich syndrome protein and scar homolog (WASH) complex is the major Arp2/3 activator at the surface of endosomes. The branched actin network, that the WASH complex induces, contributes to cargo sorting and scission of transport intermediates destined for most endosomal routes. A maj...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200038
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100099
更新日期:2012-08-01 00:00:00
abstract::The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90037-2
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040102
更新日期:2005-06-01 00:00:00
abstract::Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00577.x
更新日期:1987-01-01 00:00:00
abstract::A new class of fluorescent dye which upon excitation at 488 nm turns red is shown to be probe-suitable for using in flow cytometry alone or in conjunction with fluorescein derivatives. 7-dimethylamino 3-(p-formylstyryl) 1,4 benzoxazin 2-one is suitable for rendering microorganisms, such as Plasmodium merozoites and ce...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90340-9
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND INFORMATION:There have been several studies to understand the influence of stiffness of the culture substrates for different types of adherent cells. It is generally accepted that cell proliferation, spreading and focal adhesions increase with higher matrix stiffness. However, what remains unclear is whether...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201900115
更新日期:2020-10-01 00:00:00
abstract::The distribution of viral RNA molecules in HeLa cells infected with adenovirus type 5 (Ad5) was determined by in situ hybridization at the ultrastructural level at an intermediate stage of nuclear transformation, when viral DNA synthetic activities were maximal but progeny viruses were still sparse. Transcription site...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90060-z
更新日期:1991-01-01 00:00:00