Ultrastructural in situ hybridization: a review of technical aspects.

abstract：:In many cell types, microtubules are preferentially associated with the Golgi apparatus. However, the existence of a functional link between these two organelles is still hypothetical. To gain insight into this question, the relationships between microtubules and the Golgi apparatus were studied in rat hepatocytes dur...

journal_title：Biology of the cell

authors： Maurice M,Bellon B,Mahu JL,Feldmann G

更新日期：1983-01-01 00:00:00

abstract：:Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...

journal_title：Biology of the cell

authors： Desnos C,Huet S,Darchen F

更新日期：2007-08-01 00:00:00

abstract：:This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...

journal_title：Biology of the cell

authors： Eggena P,Buku A

更新日期：1989-01-01 00:00:00

abstract：:Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...

journal_title：Biology of the cell

authors： Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

更新日期：1988-01-01 00:00:00

abstract：:Changes in the organization of centrosomes in mouse splenic T lymphocytes stimulated by concanavalin A (con A) were examined by electron microscopy of serial sections. In both resting and stimulated lymphocytes the single centrosome consists of a pair of centrioles, satellite bodies, and pericentriolar material. In re...

journal_title：Biology of the cell

authors： Schweitzer I,Brown DL

更新日期：1984-01-01 00:00:00

abstract：:Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...

journal_title：Biology of the cell

authors： Arbona M,Cuenca JB,de Frutos R

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...

journal_title：Biology of the cell

authors： Petersson J,Ageberg M,Sandén C,Olofsson T,Gullberg U,Drott K

更新日期：2012-08-01 00:00:00

abstract：:Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, ...

journal_title：Biology of the cell

authors： Maftah A,Huet O,Gallet PF,Ratinaud MH

更新日期：1993-01-01 00:00:00

abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...

journal_title：Biology of the cell

authors： Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AA

更新日期：1987-01-01 00:00:00

abstract：:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...

journal_title：Biology of the cell

authors： Chau MF,Orias E

更新日期：1996-01-01 00:00:00

abstract：:Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...

journal_title：Biology of the cell

authors： Candy JM,Oakley AE,Mountfort SA,Taylor GA,Morris CM,Bishop HE,Edwardson JA

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND INFORMATION:CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficu...

journal_title：Biology of the cell

authors： Kolotuev I,Schwab Y,Labouesse M

更新日期：2009-12-04 00:00:00

abstract：BACKGROUND INFORMATION:Carcinoma of the oesophagus is the sixth leading cause of cancer death in the western world and is associated with a 5-year survival of less than 15%. Recent evidence suggests that stromal-epithelial interactions are fundamental in carcinogenesis. The advent of co-culture techniques permits the i...

journal_title：Biology of the cell

authors： Underwood TJ,Derouet MF,White MJ,Noble F,Moutasim KA,Smith E,Drew PA,Thomas GJ,Primrose JN,Blaydes JP

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND INFORMATION:Epithelial cadherin (E-cadherin) is involved in cell-cell adhesion through its extracellular domain, whereas the intracellular domain interacts with adaptor proteins, i.e. β-catenin, links E-cadherin to the actin cytoskeleton and participates in signal transduction events. E-cadherin protects mam...

journal_title：Biology of the cell

authors： Rosso M,Lapyckyj L,Amiano N,Besso MJ,Sánchez M,Chuluyan E,Vazquez-Levin MH

更新日期：2014-09-01 00:00:00

abstract：:Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of...

journal_title：Biology of the cell

authors： Noaillac-Depeyre J

更新日期：1985-01-01 00:00:00

abstract：:RNA helicases of the DEAD-box and related families have been found to be required for all processes involving RNA molecules. Biochemical and genetic analyses have shown that at least two RNA helicases are required for translation initiation in yeast. Although it is generally believed that these enzymes are necessary t...

journal_title：Biology of the cell

authors： Linder P

更新日期：2003-05-01 00:00:00

abstract：:Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...

journal_title：Biology of the cell

authors： Roger L,Gadea G,Roux P

更新日期：2006-03-01 00:00:00

abstract：:In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...

journal_title：Biology of the cell

authors： Monnier Z,Bahjaoui-Bouhaddi M,Bride J,Bride M,Math F,Propper A

更新日期：1999-01-01 00:00:00

abstract：:The centrosome of the slime mould Dictyostelium discoideum displays a morphology markedly different from centriolar centrosomes or yeast spindle pole bodies, while fulfilling the same conserved functions in the organization of the microtubule cytoskeleton. Recent advances suggest that the Dictyostelium centrosome may ...

journal_title：Biology of the cell

authors： Daunderer C,Schliwa M,Gräf R

更新日期：1999-05-01 00:00:00

abstract：BACKGROUND INFORMATION:At fertilization in mammalian eggs, the sperm induces a series of Ca(2+) oscillations via the production of inositol 1,4,5-trisphosphate. Increased inositol 1,4,5-trisphosphate production appears to be triggered by a sperm-derived PLCzeta (phospholipase C-zeta) that enters the egg after gamete fu...

journal_title：Biology of the cell

authors： Yu Y,Halet G,Lai FA,Swann K

更新日期：2008-11-01 00:00:00

abstract：:This study was undertaken to evaluate whether hypertrophy and hyperplasia of the pancreatic acinar cells induced by caerulein remained after termination of the hormonal treatment. Rats received subcutaneous injections of saline or caerulein for 4 days and were killed immediately after termination of treatment or 2, 15...

journal_title：Biology of the cell

authors： Morisset J,Grondin G

更新日期：1989-01-01 00:00:00

abstract：:Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage-like differentiation i.e. the pres...

journal_title：Biology of the cell

authors： Ronot X,Sene C,Boschetti E,Hartmann DJ,Adolphe M

更新日期：1984-01-01 00:00:00

abstract：:The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...

journal_title：Biology of the cell

authors： Brugmann SA,Moody SA

更新日期：2005-05-01 00:00:00

abstract：BACKGROUND INFORMATION:The endometrial epithelial cell membrane is a key interface in female reproductive biology. Steroid hormones play a predominant role in cyclic changes which occur at this interface during the female menstrual cycle. Specific changes in the morphology of the endometrial epithelial cell surface bec...

journal_title：Biology of the cell

authors： Francis LW,Lewis PD,Gonzalez D,Ryder TA,Webb G,Joels LA,White JO,Wright CJ,Conlan RS

更新日期：2009-06-04 00:00:00

abstract：:This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...

journal_title：Biology of the cell

authors： Dirksen ER

更新日期：1991-01-01 00:00:00

abstract：:Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...

journal_title：Biology of the cell

authors： Anba J,Bernadac A,Pages JM,Lazdunski C

更新日期：1984-01-01 00:00:00

abstract：:The occurrence of the tubulin post-translational modification, polyglycylation, in stable microtubular structures was investigated during morphogenesis in two ciliates, Paramecium and Frontonia atra, belonging to the Epiplasmata group. This analysis was carried out by means of immunofluorescence and post-embedding imm...

journal_title：Biology of the cell

authors： Iftode F,Clérot JC,Levilliers N,Bré MH

更新日期：2000-12-01 00:00:00

abstract：:The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

journal_title：Biology of the cell

authors： Dacosta B,Ryter A,Mounier J,Sansonetti P

更新日期：1990-01-01 00:00:00

abstract：:As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...

journal_title：Biology of the cell

authors： Crabbe J,Beaujean V,Devuyst O

更新日期：1989-01-01 00:00:00

abstract：:Flow DNA analysis was performed on samples from 71 surgically removed lung cancers and from 145 patients undergoing bronchoscopy. Abnormal DNA stem lines, characterized by their DNA index (DI), were frequently observed in operated lung carcinomas (87%). Two or three abnormal DNA stem lines were discovered simultaneous...

journal_title：Biology of the cell

authors： Chassevent A,Berruchon J,Bertrand G,Chretien MF,Tuchais C,George P,Larra F,Rebel A,Oury M

更新日期：1986-01-01 00:00:00