Flow cytometry's contribution to the measurement of cell functions.

abstract：:Revision of the reference map of the polytene chromosomes of Drosophila subobscura was started by means of the electron microscope. We present a map of regions 70B to 72D of the E chromosome obtained from squashed and thin-sectioned salivary glands. It was observed that the total number of bands in divisions 70B to 72...

journal_title：Biology of the cell

authors： Pérez-Alonso M,Cuenca JB,De Frutos R

更新日期：1988-01-01 00:00:00

abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...

journal_title：Biology of the cell

authors： N'Da E,Bonnanfant-Jais ML,Moreau N,Angelier N

更新日期：1990-01-01 00:00:00

abstract：:Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...

journal_title：Biology of the cell

authors： Gracia-Navarro F,Garcia-Navarro S,Garcia-Herdugo G

更新日期：1986-01-01 00:00:00

abstract：:This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...

journal_title：Biology of the cell

authors： Eggena P,Buku A

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...

journal_title：Biology of the cell

authors： de Andrade Rosa I,Caruso MB,de Oliveira Santos E,Gonzaga L,Zingali RB,de Vasconcelos ATR,de Souza W,Benchimol M

更新日期：2017-06-01 00:00:00

abstract：:The communication of calcium signals between cells is known to be operative between neurons where these signals integrate intimately with electrical and chemical signal communication at synapses. Recently, it has become clear that glial cells also exchange calcium signals between each other in cultures and in brain sl...

journal_title：Biology of the cell

authors： Braet K,Cabooter L,Paemeleire K,Leybaert L

更新日期：2004-02-01 00:00:00

abstract：:Gap junctions are widely expressed in the various cell types of the central nervous system. These specialized membrane intercellular junctions provide the morphological support for direct electrical and biochemical communication between adjacent cells. This intercellular coupling is controlled by neurotransmitters and...

journal_title：Biology of the cell

authors： Rouach N,Avignone E,Même W,Koulakoff A,Venance L,Blomstrand F,Giaume C

更新日期：2002-11-01 00:00:00

abstract：:A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rathe...

journal_title：Biology of the cell

authors： Amos WB,White JG

更新日期：2003-09-01 00:00:00

abstract：:The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to sync...

journal_title：Biology of the cell

authors： Helenius A

更新日期：1984-01-01 00:00:00

abstract：:Background information. In vitro-cultured microspores, after an appropriate stress treatment, can switch towards an embryogenic pathway. This process, known as microspore embryogenesis, is an important tool in plant breeding. Basic studies on this process in economically interesting crops, especially in recalcitrant p...

journal_title：Biology of the cell

authors： Bárány I,González-Melendi P,Fadón B,Mitykó J,Risueño MC,Testillano PS

更新日期：2005-09-01 00:00:00

abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...

journal_title：Biology of the cell

authors： Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AA

更新日期：1987-01-01 00:00:00

abstract：:The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...

journal_title：Biology of the cell

authors： Brugmann SA,Moody SA

更新日期：2005-05-01 00:00:00

abstract：:Circulating glucocorticoid (GC) levels are thought to modulate the basal activity of pro-opiomelanocortin (POMC) neurons within the mediobasal hypothalamus (MBH) of the male rat. In a recent study we demonstrated that Fos-immunoreactivity (Fos-IR) was spontaneously induced throughout the dark phase of the light/dark c...

journal_title：Biology of the cell

authors： Jamali KA,Tramu G

更新日期：1997-12-01 00:00:00

abstract：:Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal degenerative diseases, characterized by the progressive death of rod and cone photoreceptors. A tremendous genetic heterogeneity is associated with the RP phenotype. Most mutations affect rods selectively and, through an unknown pathway, cause the...

journal_title：Biology of the cell

authors： Delyfer MN,Léveillard T,Mohand-Saïd S,Hicks D,Picaud S,Sahel JA

更新日期：2004-05-01 00:00:00

abstract：:Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...

journal_title：Biology of the cell

authors： Candy JM,Oakley AE,Mountfort SA,Taylor GA,Morris CM,Bishop HE,Edwardson JA

更新日期：1992-01-01 00:00:00

abstract：:Interactions of cells with the extracellular matrix are essential for the control of tissue remodelling, cell migration, and embryogenesis. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where transmembrane adhesion receptors provide a structural link bet...

journal_title：Biology of the cell

authors： Petit V,Thiery JP

更新日期：2000-10-01 00:00:00

abstract：:Background information. Transport of water and small neutral solutes across plasma membranes is facilitated by AQP (aquaporin) and aquaglyceroporin channels, which belong to the MIP (major intrinsic protein) family. So far, more than 800 MIP proteins have been identified on the basis of sequence homology, but only les...

journal_title：Biology of the cell

authors： Hubert JF,Duchesne L,Delamarche C,Vaysse A,Gueuné H,Raguénès-Nicol C

更新日期：2005-09-01 00:00:00

abstract：BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...

journal_title：Biology of the cell

authors： Buschke DG,Squirrell JM,Fong JJ,Eliceiri KW,Ogle BM

更新日期：2012-06-01 00:00:00

abstract：:An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...

journal_title：Biology of the cell

authors： Cosson B,Berkova N,Couturier A,Chabelskaya S,Philippe M,Zhouravleva G

更新日期：2002-09-01 00:00:00

abstract：:A new class of fluorescent dye which upon excitation at 488 nm turns red is shown to be probe-suitable for using in flow cytometry alone or in conjunction with fluorescein derivatives. 7-dimethylamino 3-(p-formylstyryl) 1,4 benzoxazin 2-one is suitable for rendering microorganisms, such as Plasmodium merozoites and ce...

journal_title：Biology of the cell

authors： Monsigny M,Midoux P,Le Bris MT,Roche AC,Valeur B

更新日期：1989-01-01 00:00:00

abstract：:Alpha 2-macroglobulin (alpha 2M) is a plasma inhibitor of proteinases, the steric mechanism of which is based on a considerable conformational change. The typical and distinct H-like shape of alpha 2M-chymotrypsin (alpha 2M-chy) complexes seen by electron microscopy led us to an ultrastructural study of the binding of...

journal_title：Biology of the cell

authors： Boisset N,Taveau JC,Barray M,Van Leuven F,Delain E,Lamy JN

更新日期：1988-01-01 00:00:00

abstract：:Serial sections analysis of spermatocytes of the rodent Phyllotis darwini, reveal a persistent positional relationship between nucleolar material and centrioles. Pore rich areas interposed between these nucleocytoplasmic components, are represented graphically. Centriole replication takes place during the pachytene st...

journal_title：Biology of the cell

authors： Couve E

更新日期：1984-01-01 00:00:00

abstract：:We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...

journal_title：Biology of the cell

authors： Mohr M,Kolb H,Kolb-Bachofen V,Schlepper-Schäfer J

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...

journal_title：Biology of the cell

authors： Zimowska M,Swierczynska M,Ciemerych MA

更新日期：2013-08-01 00:00:00

abstract：:Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the tw...

journal_title：Biology of the cell

authors： Genty N,Salesse R,Garnier J

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family ...

journal_title：Biology of the cell

authors： Petrie RJ,Zhao B,Bedford F,Lamarche-Vane N

更新日期：2009-02-01 00:00:00

abstract：BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...

journal_title：Biology of the cell

authors： Li Y,Manaligod JM,Weeks DL

更新日期：2010-02-17 00:00:00

abstract：:This review highlights new developments in miRNA as diagnostic and surveillance tools in diseases damaging the renal proximal tubule mediated by endothelin in the field of renal carcinoma, proteinuric kidney disease and tubulotoxicity. A new mechanism in the miRNA regulation of proteins leads to the binding of the miR...

journal_title：Biology of the cell

authors： Fries JWU

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND INFORMATION:AQP4 (aquaporin 4) internalization and a concomitant decrease in the osmotic water permeability coefficient (Pf) after histamine exposure has been reported in AQP4-transfected gastric HGT1 cells. RESULTS:In the present study we report that AQP4 internalization is followed by an increase in AQP4 ...

journal_title：Biology of the cell

authors： Carmosino M,Procino G,Tamma G,Mannucci R,Svelto M,Valenti G

更新日期：2007-01-01 00:00:00

abstract：:Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change...

journal_title：Biology of the cell

authors： Gallagher BC,Moody SA

更新日期：2004-09-01 00:00:00