Abstract：
:Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop" shape of MB cytoskeletons in unfixed suspensions where the microtubule coils had a mean diameter of 2.87 microns (+/- 0.18 micron, SD). Microtubules were uncoiled by brief exposure to trypsin (2 ng/micrograms protein) or by NaCl (154-600 mM) but not by DNase I, which removed approximately 40% of total actin, but had no effect on dark-field images of microtubule coils. As microtubules uncoiled, a single fiber emerged from the hoop and gradually lengthened as the brightness of the hoop diminished; these fibers correspond to the single microtubules seen by EM. Polypeptides of coiled and uncoiled MB cytoskeletons were analyzed by SDS-PAGE. When microtubules became uncoiled, no changes in the major components (alpha- and beta-tubulin, IEF-51K, or actin) were found. However, a number (greater than 10) of minor polypeptides, each less than 5% of total cytoskeletal protein and with an Mr ranging from 80,000- greater than 260,000, were decreased in "uncoiled" MB cytoskeletons. These results implicate one or more of these minor polypeptides in maintenance of hoop integrity. Dark-field light microscopy thus provides an approach toward investigating the mechanism(s) involved in maintaining the microtubule coil of the platelet marginal band.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Kowit JD,Linck RW,Kenney DMdoi
10.1016/0248-4900(88)90002-0subject
Has Abstractpub_date
1988-01-01 00:00:00pages
283-91issue
3eissn
0248-4900issn
1768-322Xjournal_volume
64pub_type
杂志文章abstract：:The daily rhythm in the activity of arylalkylamine N-acetyltransferase (AA-NAT) controls the rhythm in melatonin synthesis in the pineal gland. In the rat, transcriptional regulatory mechanisms play a major role in determining the observed pattern of AA-NAT gene expression. Remarkably, high levels of AA-NAT transcript...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1993.tb00899.x
更新日期：1993-01-01 00:00:00
abstract：:The nucleolar lesions provoked by the action of ribonuclease (RNase) on living chick embryo fibroblasts were studied by means of microcinematographic analysis and at the ultrastructural level using oxidized diaminobenzidine as a differentially contrasting stain for nucleic acids. This study has shown that the inductio...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00218.x
更新日期：1983-01-01 00:00:00
abstract：:cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90134-m
更新日期：1992-01-01 00:00:00
abstract：:The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the laye...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)84780-0
更新日期：1996-01-01 00:00:00
abstract：:Regulated exocytosis of defensive secretory organelles, the trichocysts, as well as a transient Ca(2+)-influx can be induced in Paramecium by aminoethyldextran (Kerboeuf and Cohen, J Cell Biol (1990) 111, 2527). Knoll et al (Febs Lett (1992) 304, 265) reported that veratridine was also a secretagogue for Paramecium. H...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1996.tb00953.x
更新日期：1996-01-01 00:00:00
abstract：:beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20040531
更新日期：2005-12-01 00:00:00
abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1991.tb03015.x
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND INFORMATION:ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20100145
更新日期：2011-08-01 00:00:00
abstract：:In mature ciliated cells, the basal feet associated to the basal bodies point out in the direction of the effective stroke of the ciliary beating. In contrast, during ciliogenesis, the basal feet of the newly anchored basal bodies are randomly oriented. The reorientation of basal bodies occurs during the beginning of ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00417.x
更新日期：1985-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Caspase-dependent and -independent death mechanisms are involved in apoptosis in a variety of human carcinoma cells treated with antineoplastic compounds. Our laboratory has reported that p53 is a key contributor of mitochondrial apoptosis in cervical carcinoma cells after staurosporine exposure....
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080164
更新日期：2009-06-04 00:00:00
abstract：:A quantitative ultrastructural study was performed on 56 ejaculates showing anomalies of the sperm axonemal complex. The anomalies comprised either the absence of one, or more often several, axonemal structures, or defective elongation of the doublets. Several characteristics relating to the extent and superimposition...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00253.x
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Homotypic internalisation of tumour cells has frequently been observed in tumour tissue sections. Events of non-professional phagocytosis, however, may also occur in normal tissue if the number of dying cells exceeds the phagocytic capacity of professional phagocytes such as macrophages and dendr...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201400090
更新日期：2015-10-01 00:00:00
abstract：BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070091
更新日期：2008-03-01 00:00:00
abstract：:Photoperiodic control of several biological rhythms is exerted through the inhibitory effect of light on melatonin synthesis in the pineal organ. Hydroxyindole-O-methyltransferase (HIO-MT), the last acting-enzyme in melatonin biosynthesis, constitutes a specific marker of melatoninergic cells. In the present study, an...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(88)90098-6
更新日期：1988-01-01 00:00:00
abstract：:Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00574.x
更新日期：1987-01-01 00:00:00
abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90362-7
更新日期：1990-01-01 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(02)00006-0
更新日期：2002-10-01 00:00:00
abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90340-9
更新日期：1990-01-01 00:00:00
abstract：:Ciliary receptor cells, typified by cilia or modified cilia, are very common in the animal kingdom. In addition to the cytoskeleton of their ciliary processes these receptors possess other specific prominent cytoskeletal elements. Two representative systems are presented: i) scolopidia, mechanosensitive sensilla of va...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90441-3
更新日期：1992-01-01 00:00:00
abstract：:Oocyte maturation and early development have been intensively studied for well over 100 years. The earliest theory proposed that after fertilisation and during cell division determinants were unequally distributed to control cell fate; experimental proof came from using frog eggs (Roux, 1888). After understanding the ...
journal_title：Biology of the cell
pub_type： 传,社论,历史文章
doi：10.1016/j.biolcel.2004.01.002
更新日期：2004-04-01 00:00:00
abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90369-e
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND INFORMATION:In eukaryotic cells, autophagy is considered a lysosomal catabolic process which participates in the degradation of intracellular components in a vacuolar structure termed autolysosome. This pathway plays a significant role in the erythropoiesis process, contributing to the clearance of some orga...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201500058
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND INFORMATION:Autofluorescence spectroscopy is a powerful tool for molecular histology and for following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, it is mostly limited to visible and near infrared excitation of the samples. Several interestin...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201200075
更新日期：2013-07-01 00:00:00
abstract：:The dodecasatellite is a 11/12 bp tandemly repeated sequence which is overrepresented, with regard to its genomic representation, in extrachromosomal circular DNAs from D melanogaster embryos. Here we show that a bacterial plasmid carrying a cluster of dodecasatellite is not able to replicate efficiently in cultured c...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90262-d
更新日期：1993-01-01 00:00:00
abstract：:Structured mRNA elements called riboswitches control gene expression by binding to small metabolites. Over a dozen riboswitch classes have been characterized that target a broad range of molecules and vary widely in size and secondary structure. Four of the known riboswitch classes recognize purines or modified purine...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20070088
更新日期：2008-01-01 00:00:00
abstract：:Brain intricacies and the difficulty that scientists encounter in revealing its function with standard approaches such as electrical stimulation of neurons have led to the exploration of new tools that enable the study of neural circuits in a remote and non-invasive way. To this end, optogenetics has initialised a rev...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201200087
更新日期：2013-10-01 00:00:00
abstract：:The p126 protein is synthesized by P. falciparum between the 32nd and the 36th hour of the erythrocytic cycle, and is localized in the parasitophorous vacuole. It is processed when schizonts rupture and the major fragments (50, 47 and 18 kDa), which are released into culture supernatant, have been characterized using ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(88)90080-9
更新日期：1988-01-01 00:00:00
