Abstract:
BACKGROUND:The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus-encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of several novel proteins in association with a relatively conserved protein TbTim17. Tandem affinity purification of the TbTim17 protein complex revealed TbTim54 as a potential component of this complex. RESULTS:TbTim54, a trypanosome-specific IMS protein, is peripherally associated with the IM and is present in a protein complex slightly larger than the TbTim17 complex. TbTim54 knockdown (KD) reduced the import of TbTim17 and compromised the integrity of the TbTim17 complex. TbTim54 KD inhibited the in vitro mitochondrial import and assembly of the internal signal-containing mitochondrial carrier proteins MCP3, MCP5 and MCP11 to a greater extent than TbTim17 KD. Furthermore, TbTim54 KD, but not TbTim17 KD, significantly hampered the mitochondrial targeting of ectopically expressed MCP3 and MCP11. These observations along with our previous finding that the mitochondrial import of N-terminal signal-containing proteins like cytochrome oxidase subunit 4 and MRP2 was affected to a greater extent by TbTim17 KD than TbTim54 KD indicating a substrate-specificity of TbTim54 for internal-signal containing mitochondrial proteins. In other organisms, small Tim chaperones in the IMS are known to participate in the translocation of MCPs. We found that TbTim54 can directly interact with at least two of the six known small TbTim proteins, TbTim11 and TbTim13, as well as with the N-terminal domain of TbTim17. CONCLUSION:TbTim54 interacts with TbTim17. It also plays a crucial role in the mitochondrial import and complex assembly of internal signal-containing IM proteins in T. brucei. SIGNIFICANCE:We are the first to characterise TbTim54, a novel TbTim that is involved primarily in the mitochondrial import of MCPs and TbTim17 in T. brucei.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Singha UK,Tripathi A,Smith JT Jr,Quinones L,Saha A,Singha T,Chaudhuri Mdoi
10.1111/boc.202000054subject
Has Abstractpub_date
2020-10-20 00:00:00eissn
0248-4900issn
1768-322Xpub_type
杂志文章abstract::During a historical research, we realized that René Couteaux (1909-1999) was the first histologist who stained the postsynaptic structure of the neuromuscular junction. By means of Janus Green B dye, he revealed the membranous 'subneural apparatus' related to the 'synaptic gutter'. Hence, he justified the use of the p...
journal_title:Biology of the cell
pub_type: 传,历史文章,杂志文章
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更新日期:2006-08-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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doi:10.1016/0248-4900(94)90066-3
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00523.x
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80017-0
更新日期:1994-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90313-r
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0248-4900(05)80196-0
更新日期:1993-01-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1996.tb00988.x
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00383.x
更新日期:1985-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90060-z
更新日期:1991-01-01 00:00:00
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更新日期:1999-05-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2011-10-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300013
更新日期:2013-07-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00558.x
更新日期:1987-01-01 00:00:00