Sites of transcription of adenovirus type 5 genomes in relation to early viral DNA replication in infected HeLa cells. A high resolution in situ hybridization and autoradiographical study.

Abstract:

:The distribution of viral RNA molecules in HeLa cells infected with adenovirus type 5 (Ad5) was determined by in situ hybridization at the ultrastructural level at an intermediate stage of nuclear transformation, when viral DNA synthetic activities were maximal but progeny viruses were still sparse. Transcription sites of the viral DNA were localized by short pulse, high resolution autoradiography. Nascent viral RNA was found mainly within the nuclear compartment identified at the peripheral replicative zone, which is known to be the main replicative site of Ad5 viral genomes. Viral RNA molecules also were present, but to a markedly lesser extent, within the contiguous single-stranded (ss) DNA accumulation site, another intranuclear virus-induced structure in which some replication of viral genomes also occurs. Two other virus-induced nuclear structures contained viral RNA, the occasional exceptionally enlarged clusters of interchromatin granules and the compact rings, both DNA-free structures of unknown significance but which might play a role in the process of maturation of the Ad5 primary transcripts. Viral messenger RNA molecules were localized over the large areas of the cytoplasm which contain numerous ribosomes. Our analysis of the effects of various enzymatic pretreatments of the sections of infected cells on the revelation of nascent RNA by in situ hybridization is reviewed.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Puvion-Dutilleul F,Puvion E

doi

10.1016/0248-4900(91)90060-z

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

135-47

issue

1-2

eissn

0248-4900

issn

1768-322X

pii

0248-4900(91)90060-Z

journal_volume

71

pub_type

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