Abstract:
:We studied the behaviour in interphase and mitotic human cells of a 125 kDa (pI 6.5) antigen, associated with the nuclear matrix and detected in proliferating cells. Indirect immunofluorescence with a specific monoclonal antibody reveals that during interphase in WISH and Namalwa cells, as well as phytohaemagglutinin-stimulated lymphocytes, the antigen displays a speckled distribution in the nucleoplasm of all cells. At early prophase the fluorescence intensity of the coalesced speckles increases markedly. During metaphase and anaphase the antigen gives maximal fluorescence distributed diffusely in the nucleoplasm, while chromosomes remain negative. At anaphase and cytokinesis the antigen is still cytoplasmic, but fluorescence intensity decreases. Two-dimensional gel electrophoresis and immunoblotting reveal that the p125/6.5 antigen displays a net increase in isolated mitotic cells as compared to interphase cells. These results suggest that the p125/6.5 protein participates in late G2 phase and G2/M transition events preparing the cell for mitosis.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Todorov IT,Philipova PN,Zhelev NZ,Hadjiolov AAsubject
Has Abstractpub_date
1988-01-01 00:00:00pages
105-10issue
2eissn
0248-4900issn
1768-322Xpii
0248-4900(88)90026-3journal_volume
62pub_type
杂志文章abstract::In mature ciliated cells, the basal feet associated to the basal bodies point out in the direction of the effective stroke of the ciliary beating. In contrast, during ciliogenesis, the basal feet of the newly anchored basal bodies are randomly oriented. The reorientation of basal bodies occurs during the beginning of ...
journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1987-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1998-12-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00274.x
更新日期:1984-01-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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更新日期:1988-01-01 00:00:00
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