TPA induces apoptosis in MPC-11 mouse plasmacytoma cells grown in serum-free medium.

Abstract:

:Apoptotic-like events could be rapidly induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in cells of the mouse plasmacytoma cell line MPC-11 grown in serum-free medium. Indicators for apoptosis were morphological changes visualized by light and electron microscopy, such as chromatin condensation and the formation of cellular buds and fragments, as well as biochemical indices like the appearance of the so-called 'DNA ladder'. Additionally, in these cells which are usually devoid of significant amounts of cytoplasmic intermediate filament (cIF) proteins, synthesis and accumulation of the cIF protein vimentin was rapidly induced by TPA treatment and almost all cells became vimentin-positive. Later on, substantial amounts of vimentin and lamin B degradation products appeared, and an increasing fraction of cells displayed low or even undetectable quantities of intact vimentin. This subpopulation was characterized via microscopy to be in the late stages of apoptosis. We suggest that in MPC-11 cells undergoing apoptosis in response to TPA treatment vimentin as well as lamin B are degraded, leading to a rearrangement and eventual loss of their respective filament networks.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Giese G,Schmidt J,Gilbert M,Albrecht R,Traub P

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

99-111

issue

2

eissn

0248-4900

issn

1768-322X

pii

S0248490099800690

journal_volume

89

pub_type

杂志文章
  • Dynamics of pancreatic tissue cells in the rat exposed to long-term caerulein treatment. 2. Comparative analysis of the various cell types and their growth.

    abstract::This study was undertaken to evaluate the effects of caerulein, a CCK analog, on the different cell populations of the pancreatic tissue and their respective turnover. Rats received saline or caerulein subcutaneously and 3H-thymidine intraperitoneally three times a day for 4 days. They were sacrificed immediately afte...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Morisset J,Grondin G

    更新日期:1989-01-01 00:00:00

  • Integrin diversity brings specificity in mechanotransduction.

    abstract::Cells sense and respond to the biochemical and physical properties of the extracellular matrix (ECM) through adhesive structures that bridge the cell cytoskeleton and the surrounding environment. Integrin-mediated adhesions interact with specific ECM proteins and sense the rigidity of the substrate to trigger signalli...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/boc.201700060

    authors: Seetharaman S,Etienne-Manneville S

    更新日期:2018-03-01 00:00:00

  • Effects of acidic pH on the formation of vinblastine-induced paracrystals in Chinese hamster ovary cells.

    abstract::The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(94)90066-3

    authors: Takanari H,Morita J,Yamanaka H,Yada K,Takahashi A,Izutsu K

    更新日期:1994-01-01 00:00:00

  • Vascular cell lines expressing SSAO/VAP-1: a new experimental tool to study its involvement in vascular diseases.

    abstract:BACKGROUND INFORMATION:PrAO (primary amine oxidase), also known as SSAO (semicarbazide-sensitive amine oxidase)/VAP-1 (vascular adhesion protein-1), is an enzyme (EC 1.4.3.21) that is highly expressed in blood vessels and participates in many cell processes, including glucose handling or inflammatory leucocyte recruitm...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20110049

    authors: Solé M,Unzeta M

    更新日期:2011-11-01 00:00:00

  • Synthesis of frog virus 3 proteins occurs on intermediate filament-bound polyribosomes.

    abstract::Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Murti K,Goorha R

    更新日期:1989-01-01 00:00:00

  • Immunodetection of lipopolysaccharide in macrophages during the processing of non invasive Shigella dysenteriae.

    abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90343-2

    authors: Dacosta B,Ryter A,Mounier J,Sansonetti P

    更新日期:1990-01-01 00:00:00

  • Changes in organization and microtubule assembly activity of the centrosome during lymphocyte stimulation.

    abstract::Changes in the organization of centrosomes in mouse splenic T lymphocytes stimulated by concanavalin A (con A) were examined by electron microscopy of serial sections. In both resting and stimulated lymphocytes the single centrosome consists of a pair of centrioles, satellite bodies, and pericentriolar material. In re...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00332.x

    authors: Schweitzer I,Brown DL

    更新日期:1984-01-01 00:00:00

  • Neuropeptide Y expression, localization and cellular transducing effects in HUVEC.

    abstract:BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040102

    authors: Silva AP,Kaufmann JE,Vivancos C,Fakan S,Cavadas C,Shaw P,Brunner HR,Vischer U,Grouzmann E

    更新日期:2005-06-01 00:00:00

  • Precursors of neuropeptides in the marine worm Nereis diversicolor. In vitro translation of a precursor related to human prepro-cholecystokinin and immunolocalization of this precursor in the nervous system.

    abstract::Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90054-q

    authors: Guissi-Kadri S,Dhainaut-Courtois N,Porchet M,Curgy JJ

    更新日期:1991-01-01 00:00:00

  • EYA1 mutations associated with the branchio-oto-renal syndrome result in defective otic development in Xenopus laevis.

    abstract:BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20090098

    authors: Li Y,Manaligod JM,Weeks DL

    更新日期:2010-02-17 00:00:00

  • The lateral line of zebrafish: a model system for the analysis of morphogenesis and neural development in vertebrates.

    abstract::The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2003.10.005

    authors: Dambly-Chaudière C,Sapède D,Soubiran F,Decorde K,Gompel N,Ghysen A

    更新日期:2003-12-01 00:00:00

  • Human papillomaviruses type 16+ and 18+ cervical carcinoma cells are sensitive to staurosporine-mediated apoptosis.

    abstract::We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(02)01220-0

    authors: Bernard B,Prétet JL,Charlot JF,Mougin C

    更新日期:2003-01-01 00:00:00

  • Egg activation: upstream of the fertilization calcium signal.

    abstract::Interaction of sperm and egg at fertilization induces well-coordinated molecular events including specific recognition between species, adhesion and fusion, that lead to the formation of a zygote, a totipotent cell that develops into a new individual. A calcium signal, common to a great number of species, from marine ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(00)01065-0

    authors: Ciapa B,Chiri S

    更新日期:2000-07-01 00:00:00

  • Compartmentalized DCC signalling is distinct from DCC localized to lipid rafts.

    abstract:BACKGROUND INFORMATION:Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070108

    authors: Petrie RJ,Zhao B,Bedford F,Lamarche-Vane N

    更新日期:2009-02-01 00:00:00

  • Ultrastructural changes in the pars intermedia of the goldfish kept in calcium-free environments.

    abstract::The PAS-positive-calcium-sensitive (Ca-s) cells of the pars intermedia (PI) were studied in goldfish kept in fresh water (FW), deionized water (DW), 1/3 sea water (SW) and 1/3 Ca-free SW. Ultrastructural studies show that Ca-s cells of control goldfish kept in FW have a low activity with elongated or deeply indented n...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1984.tb00202.x

    authors: Olivereau M,Chambolle P,Dubourg P

    更新日期:1983-01-01 00:00:00

  • VICKZ proteins: a multi-talented family of regulatory RNA-binding proteins.

    abstract::The highly conserved VICKZ (Vg1 RBP/Vera, IMP-1,2,3, CRD-BP, KOC, ZBP-1) family of RNA-binding proteins recognize specific cis-acting elements in a variety of different RNAs and have been implicated in cell polarity and migration, cell proliferation, and cancer. In just the last two years, the use of transgenic mice, ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20040151

    authors: Yisraeli JK

    更新日期:2005-01-01 00:00:00

  • Characterization of cell types in human breast tumor primary cultures.

    abstract::Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00574.x

    authors: Muller D,Fricker JP,Millon-Collard R,Abecassis J,Pusel J,Eber M,Methlin G

    更新日期:1987-01-01 00:00:00

  • Synthesis of melatonin by the pineal modified photoreceptors of birds immunocytochemical localization of hydroxyindole-O-methyltransferase.

    abstract::Photoperiodic control of several biological rhythms is exerted through the inhibitory effect of light on melatonin synthesis in the pineal organ. Hydroxyindole-O-methyltransferase (HIO-MT), the last acting-enzyme in melatonin biosynthesis, constitutes a specific marker of melatoninergic cells. In the present study, an...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(88)90098-6

    authors: Guerlotte J,Voisin P,Brisson P,Faure JP,Collin JP

    更新日期:1988-01-01 00:00:00

  • Polarity reversal of inside-out thyroid follicles cultured within collagen gel: an ultrastructural study.

    abstract::Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1986.tb00463.x

    authors: Barriere H,Chambard M,Mauchamp J,Gabrion J

    更新日期:1986-01-01 00:00:00

  • Distribution of microtubules during the breakdown of the nuclear envelope of the Xenopus oocyte: an immunocytochemical study.

    abstract::Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stag...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1986.tb00448.x

    authors: Jessus C,Huchon D,Ozon R

    更新日期:1986-01-01 00:00:00

  • Functional hepatitis C virus envelope glycoproteins.

    abstract::Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that are released from HCV polyprotein by signal peptidase cleavage. These proteins assemble as a noncovalent heterodimer that is retained in the endoplasmic reticulum. The transmembrane domains of E1 and E2 are multifunctional and play a major rol...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2004.03.008

    authors: Voisset C,Dubuisson J

    更新日期:2004-08-01 00:00:00

  • F3/contactin, a neuronal cell adhesion molecule implicated in axogenesis and myelination.

    abstract::A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(02)00006-0

    authors: Falk J,Bonnon C,Girault JA,Faivre-Sarrailh C

    更新日期:2002-10-01 00:00:00

  • Progesterone induces nano-scale molecular modifications on endometrial epithelial cell surfaces.

    abstract:BACKGROUND INFORMATION:The endometrial epithelial cell membrane is a key interface in female reproductive biology. Steroid hormones play a predominant role in cyclic changes which occur at this interface during the female menstrual cycle. Specific changes in the morphology of the endometrial epithelial cell surface bec...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20080189

    authors: Francis LW,Lewis PD,Gonzalez D,Ryder TA,Webb G,Joels LA,White JO,Wright CJ,Conlan RS

    更新日期:2009-06-04 00:00:00

  • 'Should I stay or should I go?': myosin V function in organelle trafficking.

    abstract::Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20070021

    authors: Desnos C,Huet S,Darchen F

    更新日期:2007-08-01 00:00:00

  • Expression and localization of two isoforms of AQP10 in human small intestine.

    abstract:BACKGROUND INFORMATION:AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino aci...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040091

    authors: Li H,Kamiie J,Morishita Y,Yoshida Y,Yaoita E,Ishibashi K,Yamamoto T

    更新日期:2005-11-01 00:00:00

  • The periseptal annulus in Escherichia coli.

    abstract::Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1984.tb00274.x

    authors: Anba J,Bernadac A,Pages JM,Lazdunski C

    更新日期:1984-01-01 00:00:00

  • Changes in a nuclear matrix antigen during the cell cycle: interphase and mitotic cells.

    abstract::We studied the behaviour in interphase and mitotic human cells of a 125 kDa (pI 6.5) antigen, associated with the nuclear matrix and detected in proliferating cells. Indirect immunofluorescence with a specific monoclonal antibody reveals that during interphase in WISH and Namalwa cells, as well as phytohaemagglutinin-...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Todorov IT,Philipova PN,Zhelev NZ,Hadjiolov AA

    更新日期:1988-01-01 00:00:00

  • The p53 target gene TRIM22 directly or indirectly interacts with the translation initiation factor eIF4E and inhibits the binding of eIF4E to eIF4G.

    abstract:BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201100099

    authors: Petersson J,Ageberg M,Sandén C,Olofsson T,Gullberg U,Drott K

    更新日期:2012-08-01 00:00:00

  • Histochemical and biochemical studies of cholinesterases in the carotid labyrinth of amphibians.

    abstract::Acetylcholinesterase activity was detected in the carotid labyrinth of amphibians by both biochemical and histochemical methods. The histochemical tests showed enzyme activity in surfaces of afferent and efferent nerve terminals in contact with type I cells, but none within the type I cells. Acetylcholinesterase activ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00374.x

    authors: Poullet M,Verdiere M

    更新日期:1985-01-01 00:00:00

  • Expression of the lactotransferrin receptor during the differentiation process of the megakaryocyte Dami cell line.

    abstract::In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(94)80017-0

    authors: Nillesse N,Pierce A,Lecocq M,Benaissa M,Spik G

    更新日期:1994-01-01 00:00:00