Abstract：
:In 2001, with-no-lysine (WNK) kinases were identified as the genes responsible for the human hereditary hypertensive disease pseudohypoaldosteronism type II (PHAII). It took a further 6 years to clarify that WNK kinases participate in a signaling cascade with oxidative stress-responsive gene 1 (OSR1), Ste20-related proline-alanine-rich kinase (SPAK), and thiazide-sensitive NaCl cotransporter (NCC) in the kidney and the constitutive activation of this signaling cascade is the molecular basis of PHAII. Since this discovery, the WNK-OSR1/SPAK-NCC signaling cascade has been shown to be involved not only in PHAII but also in the regulation of blood pressure under normal and pathogenic conditions, such as hyperinsulinemia. However, the molecular mechanisms of WNK kinase regulation by dietary and hormonal factors and by PHAII-causing mutations remain poorly understood. In 2012, two additional genes responsible for PHAII, Kelch-like 3 (KLHL3) and Cullin3, were identified. At the time of their discovery, the molecular mechanisms underlying the interaction between these genes and their involvement in PHAII were unknown. Here we review the pathophysiological roles of the WNK signaling cascade clarified to date and introduce a new mechanism of WNK kinase regulation by KLHL3 and Cullin3, which provides insight on previously unknown mechanisms of WNK kinase regulation.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Uchida S,Sohara E,Rai T,Sasaki Sdoi
10.1111/boc.201300069subject
Has Abstractpub_date
2014-02-01 00:00:00pages
45-56issue
2eissn
0248-4900issn
1768-322Xjournal_volume
106pub_type
杂志文章,评审abstract：:Image cytometry was applied to study the intracellular localization of autofluorescence and the influence of an oxidative stress on this emission. K562 erythroleukemia cancer cells were analyzed with a microspectrofluorometer, coupled with a Argon laser (Ar+) (363 nm). From each cell, 15 x 15 emission spectra were rec...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(01)01135-2
更新日期：2001-11-01 00:00:00
abstract：:The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to sync...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00297.x
更新日期：1984-01-01 00:00:00
abstract：:Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)89429-9
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:Immunofluorescence microscopy revealed that protein kinase C (PKC)...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201500032
更新日期：2015-09-01 00:00:00
abstract：:Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction)...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00402.x
更新日期：1985-01-01 00:00:00
abstract：:Previous studies suggest that oligodeoxynucleotide (ODN) cellular uptake is cell cycle-dependent which may have important implications in cancer cell targeting. To further our understanding of ODN transport and activity, this study examines the relationships between the cell cycle, ODN cellular uptake, intracellular t...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(94)80017-0
更新日期：1994-01-01 00:00:00
abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1990.tb00007.x
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090098
更新日期：2010-02-17 00:00:00
abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(05)80189-3
更新日期：1993-01-01 00:00:00
abstract：:The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00481.x
更新日期：1986-01-01 00:00:00
abstract：:In many cell types, microtubules are preferentially associated with the Golgi apparatus. However, the existence of a functional link between these two organelles is still hypothetical. To gain insight into this question, the relationships between microtubules and the Golgi apparatus were studied in rat hepatocytes dur...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00222.x
更新日期：1983-01-01 00:00:00
abstract：:The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90052-3
更新日期：1994-01-01 00:00:00
abstract：:When mouse peritoneal macrophages were made to adhere firmly on glass surface and then removed by sequential treatment with hypotonic triethanolamine and Nonidet P-40, a set of proteins were found to be left behind at the sites of adherent cells. Such glass-adherent proteins were detected as round or ellipsoidal patch...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90200-k
更新日期：1992-01-01 00:00:00
abstract：:The highly conserved VICKZ (Vg1 RBP/Vera, IMP-1,2,3, CRD-BP, KOC, ZBP-1) family of RNA-binding proteins recognize specific cis-acting elements in a variety of different RNAs and have been implicated in cell polarity and migration, cell proliferation, and cancer. In just the last two years, the use of transgenic mice, ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040151
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND INFORMATION:In a previous study, we demonstrated that human neutrophils can develop membrane tubulovesicular extensions (TVEs) that are 160-250 nm in width and several micrometres long. These extensions, or cytonemes, are capable of establishing long-range contacts with other cells or bacteria. Cytonemes con...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201400063
更新日期：2015-05-01 00:00:00
abstract：:The internalization of the yolk proteins has been investigated by electron microscopy and cytochemistry in the oocyte of the trout which stores up large quantities of yolk. The oocyte evolution has been followed for 18 months in a homogeneous group of animals. Anionic ferritin has been injected during vitellogenesis. ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00283.x
更新日期：1984-01-01 00:00:00
abstract：:The interaction of cells with extracellular matrix components contributes to their specific differentiation. We studied hepatic peroxisomes and their changing features during embryonic development, and we immunolocalized in the same tissue the extracellular matrix components laminin and collagen IV as well as the inte...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90037-2
更新日期：1992-01-01 00:00:00
abstract：:The development of a single, very large (25-35 microns diameter) nucleolus during oogenesis in the crustacean Acanthocyclops vernalis is described. The nucleolus is the site of ribosomal RNA production in the egg, as shown by in situ hybridization, and apparently the only source, as accessory cells are not observed. R...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90340-9
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201100099
更新日期：2012-08-01 00:00:00
abstract：:Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00577.x
更新日期：1987-01-01 00:00:00
abstract：:Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20060048
更新日期：2006-11-01 00:00:00
abstract：:Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X-ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies. It is now clear that a powerful approach is t...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1994.tb00932.x
更新日期：1994-01-01 00:00:00
abstract：:Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(93)90119-y
更新日期：1993-01-01 00:00:00
abstract：:Netrins are secreted proteins that play a crucial role in neuronal migration and in axon guidance during the development of the nervous system. Netrin-1 has been shown to interact with the transmembrane receptors DCC and UNC5H and these receptors appeared of key importance in mediating the chemotropic activity of netr...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(03)00072-8
更新日期：2003-10-01 00:00:00
abstract：:Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00274.x
更新日期：1984-01-01 00:00:00
abstract：:I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90140-v
更新日期：1992-01-01 00:00:00
