Abstract:
:The daily rhythm in the activity of arylalkylamine N-acetyltransferase (AA-NAT) controls the rhythm in melatonin synthesis in the pineal gland. In the rat, transcriptional regulatory mechanisms play a major role in determining the observed pattern of AA-NAT gene expression. Remarkably, high levels of AA-NAT transcripts can only be detected in the night pineal; significant levels can also be found in the retina. To characterize the regulatory events that impinge upon the activity of the AA-NAT gene we embarked on the systematic analysis of the AA-NAT promoter. To this end we transfected several AA-NAT promoter derivative constructs to monitor reporter gene activity in both pineal and non-pineal primary cell cultures. Our studies revealed a cooperative arrangement between upstream promoter and downstream intronic regions which appear to contain most of the key elements necessary to ensure the proper spatio-temporal pattern of AA-NAT gene expression.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Baler R,Covington S,Klein DCsubject
Has Abstractpub_date
1999-12-01 00:00:00pages
699-705issue
9eissn
0248-4900issn
1768-322Xpii
S0248-4900(00)88533-0journal_volume
91pub_type
杂志文章abstract::Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(05)80189-3
更新日期:1993-01-01 00:00:00
abstract::The PAS-positive-calcium-sensitive (Ca-s) cells of the pars intermedia (PI) were studied in goldfish kept in fresh water (FW), deionized water (DW), 1/3 sea water (SW) and 1/3 Ca-free SW. Ultrastructural studies show that Ca-s cells of control goldfish kept in FW have a low activity with elongated or deeply indented n...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00202.x
更新日期:1983-01-01 00:00:00
abstract::Background information. In vitro-cultured microspores, after an appropriate stress treatment, can switch towards an embryogenic pathway. This process, known as microspore embryogenesis, is an important tool in plant breeding. Basic studies on this process in economically interesting crops, especially in recalcitrant p...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040142
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND INFORMATION:TSPO (translocator protein), previously known as PBR (peripheral-type benzodiazepine receptor), is a ubiquitous 18 kDa transmembrane protein that participates in diverse cell functions. High-affinity TSPO ligands are best known for their ability to stimulate cholesterol transport in organs synthe...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070157
更新日期:2008-07-01 00:00:00
abstract::In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00361.x
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060105
更新日期:2007-07-01 00:00:00
abstract::Effect of estradiol-17 beta on rat vaginal epithelial cells (VEC) was studied by transmission electron microscopy. During the normal estrous cycle in adult rats keratinization and exfoliation of VEC was observed only in the estrus phase, though tonofilament bundles were seen distributed in the cytoplasm of the VEC dur...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The communication of calcium signals between cells is known to be operative between neurons where these signals integrate intimately with electrical and chemical signal communication at synapses. Recently, it has become clear that glial cells also exchange calcium signals between each other in cultures and in brain sl...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/j.biolcel.2003.10.007
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND INFORMATION:ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100145
更新日期:2011-08-01 00:00:00
abstract::Infection with Mycobacterium ulcerans results in a necrotising skin disease known as a Buruli ulcer, the pathology of which is directly linked to the bacterial production of the toxin mycolactone. Recent studies have identified the protein translocation machinery of the endoplasmic reticulum (ER) membrane as the prima...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/boc.201800030
更新日期:2018-11-01 00:00:00
abstract::Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00577.x
更新日期:1987-01-01 00:00:00
abstract::An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(02)01194-2
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND INFORMATION:Loss of sensitivity to TGF-beta1 (transforming growth factor beta1)-induced growth arrest is an important step towards malignant transformation in human epithelial cells, and Id-1 (inhibitor of differentiation or DNA binding-1) has been associated with cell proliferation and cell-cycle progressio...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060026
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND INFORMATION:Meningothelial cells (MECs) are the cellular components of the meninges protecting the brain and as such provide important barrier function for the central nervous system building the interface between neuronal tissue and the cerebrospinal fluid (CSF). MECs were previously shown to be involved in...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300013
更新日期:2013-07-01 00:00:00
abstract::Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00472.x
更新日期:1986-01-01 00:00:00
abstract::Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)89429-9
更新日期:1995-01-01 00:00:00
abstract::The membrane content in phospholipids along the secretory pathway in rat pancreatic B-cells was studied in situ by high-resolution cytochemistry, applying the recently introduced phospholipase A2-gold technique. The gold particles were mostly associated with cell membranes, and the various types of membranes were labe...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Image cytometry was applied to study the intracellular localization of autofluorescence and the influence of an oxidative stress on this emission. K562 erythroleukemia cancer cells were analyzed with a microspectrofluorometer, coupled with a Argon laser (Ar+) (363 nm). From each cell, 15 x 15 emission spectra were rec...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(01)01135-2
更新日期:2001-11-01 00:00:00
abstract::Previous studies suggest that oligodeoxynucleotide (ODN) cellular uptake is cell cycle-dependent which may have important implications in cancer cell targeting. To further our understanding of ODN transport and activity, this study examines the relationships between the cell cycle, ODN cellular uptake, intracellular t...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::The nucleolar lesions provoked by the action of ribonuclease (RNase) on living chick embryo fibroblasts were studied by means of microcinematographic analysis and at the ultrastructural level using oxidized diaminobenzidine as a differentially contrasting stain for nucleic acids. This study has shown that the inductio...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00218.x
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The Wiskott-Aldrich syndrome protein and scar homolog (WASH) complex is the major Arp2/3 activator at the surface of endosomes. The branched actin network, that the WASH complex induces, contributes to cargo sorting and scission of transport intermediates destined for most endosomal routes. A maj...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200038
更新日期:2013-05-01 00:00:00
abstract::Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1998-07-01 00:00:00
abstract::During a historical research, we realized that René Couteaux (1909-1999) was the first histologist who stained the postsynaptic structure of the neuromuscular junction. By means of Janus Green B dye, he revealed the membranous 'subneural apparatus' related to the 'synaptic gutter'. Hence, he justified the use of the p...
journal_title:Biology of the cell
pub_type: 传,历史文章,杂志文章
doi:10.1042/BC20050036
更新日期:2006-08-01 00:00:00
abstract::Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00274.x
更新日期:1984-01-01 00:00:00
abstract::The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/j.1768-322x.1991.tb03015.x
更新日期:1991-01-01 00:00:00
abstract::Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90026-w
更新日期:1992-01-01 00:00:00
abstract::A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(98)80018-x
更新日期:1998-06-01 00:00:00
abstract::Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20050058
更新日期:2006-03-01 00:00:00