Characterization of the bacterial flora isolated from a pilot-scale lagoon processing swine manure.

abstract：:Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated...

journal_title：Canadian journal of microbiology

authors： Saleena LM,Loganathan P,Rangarajan S,Nair S

更新日期：2001-02-01 00:00:00

abstract：:The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...

journal_title：Canadian journal of microbiology

authors： Zhao JS,Greer CW,Thiboutot S,Ampleman G,Hawari J

更新日期：2004-02-01 00:00:00

abstract：:Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were red...

journal_title：Canadian journal of microbiology

authors： Beam HW,Curl EA,Rodriguez-Kabana R

更新日期：1977-05-01 00:00:00

abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...

journal_title：Canadian journal of microbiology

authors： Fliermans CB,Hazen TC

更新日期：1980-02-01 00:00:00

abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...

journal_title：Canadian journal of microbiology

authors： Abelovska L,Bujdos M,Kubova J,Petrezselyova S,Nosek J,Tomaska L

更新日期：2007-04-01 00:00:00

abstract：:Melanin biosynthesis in Verticillium dahliae Kleb, was studied with mutants deficient for normal black melanin or for production of microsclerotia. Seven genetically different mutants had apparent blocks in melanin biosynthesis. Four mutants (brm-1 to -4) produced brown microsclerotia and extruded pigments into media;...

journal_title：Canadian journal of microbiology

authors： Bell AA,Puhalla JE,Tolmsoff WJ,Stipanovic RD

更新日期：1976-06-01 00:00:00

abstract：:Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

journal_title：Canadian journal of microbiology

authors： Wang W,Sun M,Liu W,Zhang B

更新日期：2008-10-01 00:00:00

abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...

journal_title：Canadian journal of microbiology

authors： Johnson KG,McDonald IJ

更新日期：1976-03-01 00:00:00

abstract：:The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysi...

journal_title：Canadian journal of microbiology

authors： Kou X,Chen Q,Ju X,Liu H,Chen W,Xue Z

更新日期：2014-11-01 00:00:00

abstract：:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...

journal_title：Canadian journal of microbiology

authors： Obaid NA,Jacobson GA,Tristram S

更新日期：2015-03-01 00:00:00

abstract：:Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular pho...

journal_title：Canadian journal of microbiology

authors： Poirier TP,Holt SC

更新日期：1983-10-01 00:00:00

abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...

journal_title：Canadian journal of microbiology

authors： Pelchat M,Lacoste L,Yang F,Lapointe J

更新日期：1998-04-01 00:00:00

abstract：:A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...

journal_title：Canadian journal of microbiology

authors： Farinha MA,Kropinski AM

更新日期：1997-03-01 00:00:00

abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...

journal_title：Canadian journal of microbiology

authors： Holowenko FM,MacKinnon MD,Fedorak PM

更新日期：2000-10-01 00:00:00

abstract：:Dense populations of anaerobic bacteria were found sequentially layered below the thermocline in two eutrophic lakes in southwest Michigan. Phase and electron microscopy of whole cells and thin sections were used to reveal the in situ morphology of the dominant members of the community. The predominant chlorophyll-con...

journal_title：Canadian journal of microbiology

authors： Caldwell DE,Tiedje JM

更新日期：1975-03-01 00:00:00

abstract：:New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...

journal_title：Canadian journal of microbiology

authors： Gauvreau D,Waring MJ

更新日期：1984-06-01 00:00:00

abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...

journal_title：Canadian journal of microbiology

authors： Nomoto K,Yokokura T,Nomoto K

更新日期：1992-08-01 00:00:00

abstract：:Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...

journal_title：Canadian journal of microbiology

authors： Syed MA,Lévesque CM

更新日期：2012-05-01 00:00:00

abstract：:A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective ...

journal_title：Canadian journal of microbiology

authors： Beuchemin-Newhouse N,Cedergen RJ

更新日期：1978-03-01 00:00:00

abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...

journal_title：Canadian journal of microbiology

authors： Monteiro RA,Souza EM,Yates MG,Pedrosa FO,Chubatsu LS

更新日期：2000-11-01 00:00:00

abstract：:Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...

journal_title：Canadian journal of microbiology

authors： Hofherr L

更新日期：1978-10-01 00:00:00

abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...

journal_title：Canadian journal of microbiology

authors： Moore M,Trevors J,Lee H,Leung KT

更新日期：2005-03-01 00:00:00

abstract：:Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...

journal_title：Canadian journal of microbiology

authors： Wenman WM,Meuser RU,Wallis PM

更新日期：1986-12-01 00:00:00

abstract：:The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...

journal_title：Canadian journal of microbiology

authors： Zhang XB,Ohta Y

更新日期：1992-07-01 00:00:00

abstract：:The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...

journal_title：Canadian journal of microbiology

authors： Jendrossek D,Backhaus M,Andermann M

更新日期：1995-01-01 00:00:00

abstract：:Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...

journal_title：Canadian journal of microbiology

authors： Sly LM,Worobec EA,Perkins RE,Phibbs PV Jr

更新日期：1993-11-01 00:00:00

abstract：:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...

journal_title：Canadian journal of microbiology

authors： Duarte M,Cottenceau G,Portrait V,Pons AM

更新日期：2001-09-01 00:00:00

abstract：:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...

journal_title：Canadian journal of microbiology

authors： Lawler GC,Weber DJ

更新日期：1977-08-01 00:00:00

abstract：:Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...

journal_title：Canadian journal of microbiology

authors： Stoll D

更新日期：2001-12-01 00:00:00

abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...

journal_title：Canadian journal of microbiology

authors： Laguerre M,Turcotte R

更新日期：1975-12-01 00:00:00