Purification and characterization of tuberculin-active components from BCG.

Abstract:

:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the starting material for each of the following fractionation steps. The physicochemical properties and the antigenic activity of the biologically active fractions have shown that a single component, or only a few ones with similar properties, possessed high tuberculin activity. These active components were proteins having relatively high molecular weights (about 72,000) and could behave as antigens.

journal_name

Can J Microbiol

authors

Laguerre M,Turcotte R

doi

10.1139/m75-290

subject

Has Abstract

pub_date

1975-12-01 00:00:00

pages

2019-27

issue

12

eissn

0008-4166

issn

1480-3275

journal_volume

21

pub_type

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