Abstract:
:The multi-drug resistant pathogen Acinetobacter baumannii displays unusual control of its SOS mutagenesis genes, as it does not encode a LexA repressor, but instead employs the UmuDAb repressor and a small DdrR protein that is uniquely found in Acinetobacter species. We used bacterial adenylate cyclase two-hybrid analyses to determine if UmuDAb and DdrR coregulation might involve physical interactions. Neither quantitative nor qualitative assays showed UmuDAb interaction with DdrR. DdrR hybrid proteins, however, demonstrated modest head-to-tail interactions in a qualitative assay. The similarity of UmuDAb to the homodimer-forming polymerase manager UmuD and LexA repressor proteins suggested that it may form dimers, which we observed. UmuDAb homodimerization required a free C-terminus, and either small truncations or addition of a histidine tag at the C-terminus abolished this homodimerization. Amino acid N100, crucial for UmuD dimer formation, was dispensable if both C-termini were free to interact. However, mutation of G124, necessary for LexA dimerization, yielded significantly less UmuDAb dimerization, even if both C-termini were free. This suggests that UmuDAb forms dimers like LexA, but may not co-regulate gene expression involving a physical association with DdrR. The homodimerization of these coregulators provides insight into a LexA-independent, coregulatory process of controlling a conserved bacterial action such as the mutagenic DNA damage response.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Cook D,Carrington J,Johnson K,Hare Jdoi
10.1139/cjm-2020-0219subject
Has Abstractpub_date
2020-11-12 00:00:00eissn
0008-4166issn
1480-3275pub_type
杂志文章abstract::Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0254
更新日期:2020-12-10 00:00:00
abstract::Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-016
更新日期:1983-01-01 00:00:00
abstract::For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-047
更新日期:2003-05-01 00:00:00
abstract::Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen up...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-228
更新日期:1977-11-01 00:00:00
abstract::Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-021
更新日期:1989-01-01 00:00:00
abstract::Among nine eluents tested, 0.5% (w/v) isoelectric casein at pH 9.0 and 0.5% (w/v) non-fat dry milk (pH 9.0) were the most efficient in eluting poliovirus type 1 (Sabin) from Eustis fine sand. However, no significant difference was found between the overall (elution followed by concentration) virus recoveries by non-fa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-130
更新日期:1979-08-01 00:00:00
abstract::Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-046
更新日期:1979-03-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was express...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0240
更新日期:2019-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0630
更新日期:2015-02-01 00:00:00
abstract::Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-043
更新日期:2006-09-01 00:00:00
abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-032
更新日期:1983-02-01 00:00:00
abstract::From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-111
更新日期:1979-06-01 00:00:00
abstract::This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0465
更新日期:2013-01-01 00:00:00
abstract::The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0239
更新日期:2014-07-01 00:00:00
abstract::Lyme disease is a tick-borne disease that is emerging in Canada. The disease is caused by spirochetes of the Lyme borreliosis group, which is expanding as new species are discovered. In Canada, Lyme disease risk has so far been assessed primarily by detection of Borrelia burgdorferi sensu stricto. Of Ixodes scapularis...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0376
更新日期:2019-02-01 00:00:00
abstract::The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-110
更新日期:2002-12-01 00:00:00
abstract::Mucor alternans(van Tieghem) was found to be a diphasic species in that it grew exclusively in the yeast-like budding phase under anaerobic conditions in the complex medium yeast extract-peptone-glucose broth and in tissue-culture medium 199. In the latter medium this growth form occurred also at 37C, at an initial pH...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-010
更新日期:1975-01-01 00:00:00
abstract::Bacterial diversity was assessed in water samples collected from several uranium mining wastes in Ger many and in the United States by using 16S rDNA and ribosomal intergenic spacer amplification retrievals. The results obtained using the 16S rDNA retrieval showed that the samples collected from the uranium mill taili...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-082
更新日期:2005-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-061
更新日期:2005-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-080
更新日期:2001-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-088
更新日期:1997-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-112
更新日期:1984-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-180
更新日期:1979-10-01 00:00:00
abstract::Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-019
更新日期:2010-04-01 00:00:00
abstract::Diversity of actinomycetes and their antifungal activities against some plant pathogenic fungi were examined in various vegetative soils from 14 different sites in the western part of Korea. Actinomycete counts ranged from 1.17 x 10(6) to 4.20 x 10(6) cfu x g(-1) dried soil. A total of 1510 actinomycetes were isolated...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-025
更新日期:2002-05-01 00:00:00
abstract::The ruminal microbiota plays major roles in feed digestion. The composition and fermentation of the bacterial communities in 3 important ruminant species have been studied previously. Here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in Tarim red deer (Cervu...
journal_title:Canadian journal of microbiology
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abstract::The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-039
更新日期:1980-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
abstract::The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00