Abstract:
:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is mediated by heme since expression was higher in a mutant defective in heme biosynthesis. KlFBA1 transcription is not induced by calcium-shortage, low temperature, or at stationary phase. These data suggest that KlFBA1 plays a role in the balance between oxidative and fermentative metabolism and that this gene is differentially regulated in K. lactis and Saccharomyces cerevisiae, i.e., a respiratory vs. fermentative yeast.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Prado SM,Cerdán ME,González Siso MIdoi
10.1139/w04-038subject
Has Abstractpub_date
2004-08-01 00:00:00pages
645-52issue
8eissn
0008-4166issn
1480-3275pii
w04-038journal_volume
50pub_type
杂志文章abstract::For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-047
更新日期:2003-05-01 00:00:00
abstract::Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-032
更新日期:2010-06-01 00:00:00
abstract::Fine hair-like appendages on the cell walls of the another smut Ustilago violacea are described. These hairs are termed fimbriae because of their close similarity to the fimbriae (pili) found on certain Gram-negative bacteria. Cells of U. violacea may carry more than 200 fimbriae varying in length from about 0.5 mum t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-076
更新日期:1975-04-01 00:00:00
abstract::Fluorescent antibody was prepared against a temperate-soil isolate of Beijerinckia obtained from a rhizosphere of rice growing in Camargue (France). The antibody did not cross-react with any of 6 species of Azotobacter, 4 species of Beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizosphere...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-023
更新日期:1977-02-01 00:00:00
abstract::Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-219
更新日期:1980-11-01 00:00:00
abstract::Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-241
更新日期:1980-12-01 00:00:00
abstract::The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-127
更新日期:1978-06-01 00:00:00
abstract::To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-197
更新日期:1977-09-01 00:00:00
abstract::Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-251
更新日期:1976-12-01 00:00:00
abstract::Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-236
更新日期:1978-12-01 00:00:00
abstract::An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-024
更新日期:1978-02-01 00:00:00
abstract::The altitudinal distribution of microfungi associated with Betula ermanii Cham. leaf litter was investigated on Mt. Rishiri (1719 m), northern Japan. Fallen leaves were collected at five sites of different altitudes (approximately 300, 600, 900, 1200, and 1500 m above sea level) along a transect on the northern slope ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-030
更新日期:2009-07-01 00:00:00
abstract::The introduction of the 7-valent pneumococcal vaccine (PCV7) in Canada was very effective in reducing invasive pneumococcal disease (IPD) in children; however, increases of non-PCV7 serotypes have subsequently offset some of these reductions. A 13-valent pneumococcal vaccine (PCV13) targeting additional serotypes was ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0614
更新日期:2013-12-01 00:00:00
abstract::This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-059
更新日期:2004-09-01 00:00:00
abstract::The multi-drug resistant pathogen Acinetobacter baumannii displays unusual control of its SOS mutagenesis genes, as it does not encode a LexA repressor, but instead employs the UmuDAb repressor and a small DdrR protein that is uniquely found in Acinetobacter species. We used bacterial adenylate cyclase t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0219
更新日期:2020-11-12 00:00:00
abstract::Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0624
更新日期:2019-09-01 00:00:00
abstract::DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-170
更新日期:1985-10-01 00:00:00
abstract::Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-020
更新日期:1988-02-01 00:00:00
abstract::The effects of water activity (aw, 0.994-0.90 identical to 0.4-14.0 (-)MPa water potential), temperature (4-45 degrees C), and pH (3.6, 5.5, 7.0), and their interactions on growth of isolates of Fusarium moniliforme and Fusarium proliferatum were determined in vitro on a maize extract agar medium. Growth of two isolat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-149
更新日期:1995-12-01 00:00:00
abstract::A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-190
更新日期:1981-11-01 00:00:00
abstract::The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-039
更新日期:1980-02-01 00:00:00
abstract::A study has been undertaken to account for the presence of Thiobacillus acidophilus in iron-grown cultures of Thiobacillus ferrooxidans. Attempts to adapt T. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of T. ferrooxidans and was able to gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-178
更新日期:1980-09-01 00:00:00
abstract::The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-105
更新日期:2007-12-01 00:00:00
abstract::The types of bacteria and fungi present in the air of a municipal solid waste recovery system have been characterized and the population densities estimated. Conventional methods were successful in enumerating coagulase-positive staphylococci, Klebsiella spp., gram-negative bacteria, fungi, and common indicator organi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-038
更新日期:1985-03-01 00:00:00
abstract::The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-021
更新日期:2007-05-01 00:00:00
abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-093
更新日期:2009-11-01 00:00:00
abstract::This study examined the phylogenetic structure of serotype a Haemophilus influenzae (Hia) isolates recovered from patients in Canada. Hia isolates from 490 separate patients and an American Type Culture Collection (ATCC) strain were analyzed by multilocus sequence typing (MLST), with 18 different sequence types (STs) ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0406
更新日期:2020-02-01 00:00:00
abstract::The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-224
更新日期:1999-03-01 00:00:00
abstract::Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-177
更新日期:1983-09-01 00:00:00