Abstract:
:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for bacterial growth. Washed (intact) cells, harvested at different periods of bacterial growth on cyanide, consumed oxygen in presence of cyanide. These findings suggest that metabolism of cyanide involved a dioxygenase enzyme that converted cyanide directly to ammonia, without the formation of cyanate.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Figueira MM,Ciminelli VS,de Andrade MC,Linardi VRdoi
10.1139/m96-070subject
Has Abstractpub_date
1996-05-01 00:00:00pages
519-23issue
5eissn
0008-4166issn
1480-3275journal_volume
42pub_type
杂志文章abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-279
更新日期:1975-12-01 00:00:00
abstract::Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-231
更新日期:1975-10-01 00:00:00
abstract::The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-290
更新日期:1975-12-01 00:00:00
abstract::Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-241
更新日期:1980-12-01 00:00:00
abstract::The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-043
更新日期:1989-02-01 00:00:00
abstract::In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-023
更新日期:2010-05-01 00:00:00
abstract::Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-050
更新日期:2005-08-01 00:00:00
abstract::Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W10-087
更新日期:2010-12-01 00:00:00
abstract::Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-099
更新日期:1991-08-01 00:00:00
abstract::Pili are essential adhesive determinants for many bacterial pathogens. A suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative "hockey-puck" colony phenotype was designed to identify novel regulators of type I pili in Serratia marcescens. Mutations that decreased pilus biosynthesis mappe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0566
更新日期:2016-01-01 00:00:00
abstract::To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-096
更新日期:2005-12-01 00:00:00
abstract::Seven tk- mutants of herpes simplex virus, type 2 (HSV-2), and three tk- mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-063
更新日期:1983-04-01 00:00:00
abstract::Diversity of actinomycetes and their antifungal activities against some plant pathogenic fungi were examined in various vegetative soils from 14 different sites in the western part of Korea. Actinomycete counts ranged from 1.17 x 10(6) to 4.20 x 10(6) cfu x g(-1) dried soil. A total of 1510 actinomycetes were isolated...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-025
更新日期:2002-05-01 00:00:00
abstract::Isolated toluene-degrading Pseudomonas stutzeri ST-9 bacteria were grown in a minimal medium containing toluene (100 mg·L(-1)) (MMT) or glucose (MMG) as the sole carbon source, with specific growth rates of 0.019 h(-1) and 0.042 h(-1), respectively. Scanning (SEM) as well as transmission (TEM) electron microscope anal...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0099
更新日期:2016-08-01 00:00:00
abstract::The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-204
更新日期:1975-09-01 00:00:00
abstract::The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0481
更新日期:2015-04-01 00:00:00
abstract::Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensifo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-078
更新日期:1980-04-01 00:00:00
abstract::The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W06-124
更新日期:2007-03-01 00:00:00
abstract::A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-055
更新日期:1978-03-01 00:00:00
abstract::Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-098
更新日期:2008-12-01 00:00:00
abstract::The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-018
更新日期:2009-06-01 00:00:00
abstract::Effects of temperature and anaerobic conditions on the replication of two bacteriophages, nt-1 and nt-6, of the estuarine bacterium Vibrio natriegens were studied. Reduction in temperature resulted in longer latent periods and reduced burst sizes for both phages. Replication under anaerobic conditions resulted in long...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-053
更新日期:1978-03-01 00:00:00
abstract::Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-182
更新日期:1978-09-01 00:00:00
abstract::Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-123
更新日期:2009-03-01 00:00:00
abstract::Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-005
更新日期:2006-06-01 00:00:00
abstract::In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0007
更新日期:2015-06-01 00:00:00
abstract::To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-189
更新日期:1992-11-01 00:00:00
abstract::Morphological differentiation has commanded attention for its putative impact on the pathogenesis of invasive fungal infections. We evaluated in vitro and in vivo the dimorphism from mycelial to yeast-phase of Sporothrix schenckii, Blastomyces dermatitidis and Paracoccidioides brasiliensis isolates, two strains for ea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-026
更新日期:2004-06-01 00:00:00
abstract::The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude ex...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W09-101
更新日期:2009-12-01 00:00:00
abstract::The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-079
更新日期:2003-10-01 00:00:00