Abstract:
:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for SED only, whereas one MAb was reactive with both SED and SEE when both SEs were used as immunogens. One MAb reacted with SEA, SED, and SEE when both SEA and SED were used as immunogens. A MAb with the highest reactivity to SED was used to prepare an immunosorbent for purification of SED by immunoaffinity chromatography. Approximately 70% of the partially purified SED was recovered in the eluate. The purified SED was electrophoretically and antigenically pure. Immunoaffinity chromatography proved useful in the purification of SED in terms of ease of purification, percent enterotoxin, and enterotoxin purity.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Shinagawa K,Omoe K,Matsusaka N,Sugii Sdoi
10.1139/m91-099subject
Has Abstractpub_date
1991-08-01 00:00:00pages
586-9issue
8eissn
0008-4166issn
1480-3275journal_volume
37pub_type
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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doi:10.1139/m81-144
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journal_title:Canadian journal of microbiology
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更新日期:2009-12-01 00:00:00
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更新日期:1988-09-01 00:00:00
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更新日期:2003-10-01 00:00:00
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journal_title:Canadian journal of microbiology
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pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
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