Attempts to inhibit ruminal methanogenesis by blocking pyruvate oxidative decarboxylation.

Abstract:

:The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythiamin decreased methanogenesis by 23%. In the third experiment, three pyruvate derivatives did not inhibit methanogenesis, although hydroxypyruvate improved organic matter fermentation from 57.8% to 64.2%. The additives did not seem to inhibit pyruvate oxidative decarboxylation.

journal_name

Can J Microbiol

authors

Ungerfeld EM,Rust SR,Burnett R

doi

10.1139/w03-079

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

650-4

issue

10

eissn

0008-4166

issn

1480-3275

pii

w03-079

journal_volume

49

pub_type

杂志文章
  • Cyanide degradation by an Escherichia coli strain.

    abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-070

    authors: Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

    更新日期:1996-05-01 00:00:00

  • Determination of peptidyl dipeptidase activity in 24 bacterial species.

    abstract::Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m90-010

    authors: Stevens J,Fanburg BL,Lanzillo JJ

    更新日期:1990-01-01 00:00:00

  • Effect of subinhibitory concentrations of antimicrobial agents (quinolones and macrolide) on the production of verotoxin by enterohemorrhagic Escherichia coli O157:H7.

    abstract::In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Yoh M,Frimpong EK,Voravuthikunchai SP,Honda T

    更新日期:1999-09-01 00:00:00

  • Design and testing of real-time PCR primers for the quantification of Methanoculleus, Methanosarcina, Methanothermobacter, and a group of uncultured methanogens.

    abstract::In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-157

    authors: Franke-Whittle IH,Goberna M,Insam H

    更新日期:2009-05-01 00:00:00

  • Chromosomal bacterial type II toxin-antitoxin systems.

    abstract::Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.1139/w2012-025

    authors: Syed MA,Lévesque CM

    更新日期:2012-05-01 00:00:00

  • Isolation and transcriptional regulation of the Kluyveromyces lactis FBA1 (fructose-1,6-bisphosphate aldolase) gene.

    abstract::Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-038

    authors: Prado SM,Cerdán ME,González Siso MI

    更新日期:2004-08-01 00:00:00

  • Molecular identification of a polyM-specific alginate lyase from Pseudomonas sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate.

    abstract::An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-106

    authors: Kam N,Park YJ,Lee EY,Kim HS

    更新日期:2011-12-01 00:00:00

  • Bindings of plasma proteins to streptococci of serological group L with special reference to their immunoglobulin G Fc-receptor activity.

    abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-001

    authors: Lämmler C,Schaufuss P,Frede C,Blobel H

    更新日期:1988-01-01 00:00:00

  • Stimulatory effect of aerosil on algal growth.

    abstract::Unicellular green alga represents not only a convenient model for its biochemical and physiological studies but also a sensitive system to test the effects of various environmental factors. Algae cells of two strains, SA-3 strain (exsymbiotic from Paramecium bursaria) and Chlorella vulgaris c-27, were asynchronously c...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w01-143

    authors: Gerashchenko BI,Gerashchenko II,Kosaka T,Hosoya H

    更新日期:2002-02-01 00:00:00

  • Synchronous sporulation during idiophase development in surface cultures of Rhizopus arrhizus (Fischer).

    abstract::A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-151

    authors: Lawler GC,Weber DJ

    更新日期:1977-08-01 00:00:00

  • The use of functional analysis of the ribosome as a tool to determine archaebacterial phylogeny.

    abstract::Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-021

    authors: Amils R,Ramírez L,Sanz JL,Marín I,Pisabarro AG,Ureña D

    更新日期:1989-01-01 00:00:00

  • Immune response and protective efficacy of S9 ribosomal protein of Streptococcus pneumoniae in a model of sepsis.

    abstract::Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-083

    authors: Araújo HF,Campos PC,Camargo DR,Pereira FN,Samuel ML,Oliveira MA,Fortes-Dias CL,Leclercq SY

    更新日期:2012-09-01 00:00:00

  • An electroporation-based system for high-efficiency transformation of germinated conidia of filamentous fungi.

    abstract::A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-147

    authors: Chakraborty BN,Patterson NA,Kapoor M

    更新日期:1991-11-01 00:00:00

  • Polygalacturonase isolated from the culture of the psychrophilic fungus Sclerotinia borealis.

    abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-059

    authors: Takasawa T,Sagisaka K,Yagi K,Uchiyama K,Aoki A,Takaoka K,Yamamato K

    更新日期:1997-05-01 00:00:00

  • The interaction of cholera toxin subunit A with cultured adrenal cells.

    abstract::Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-153

    authors: Knoop FC

    更新日期:1978-08-01 00:00:00

  • Ultraviolet germicidal irradiation disinfection of Stachybotrys chartarum.

    abstract::The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-061

    authors: Green CF,Davidson CS,Scarpino PV,Gibbs SG

    更新日期:2005-09-01 00:00:00

  • Sulfur isotope fractionation by Salmonella heidelberg: inverse isotope effects during growth on high concentrations of Na2SO3.

    abstract::During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-217

    authors: McCready RG,Krouse HR

    更新日期:1979-12-01 00:00:00

  • The effect of temperature and other growth conditions on the fatty acid composition of Escherichia coli.

    abstract::During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-128

    authors: McGarrity JT,Armstrong JB

    更新日期:1981-08-01 00:00:00

  • [Rubella virus. III. Purification of infected or non-infected Vero cell membranes].

    abstract::Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-103

    authors: Payment P,Ajdukovic D,Pavilanis V

    更新日期:1975-05-01 00:00:00

  • Cytokinin production by plant growth promoting rhizobacteria and selected mutants.

    abstract::One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w01-029

    authors: García de Salamone IE,Hynes RK,Nelson LM

    更新日期:2001-05-01 00:00:00

  • Occurrence of Vibrio parahaemolyticus in selected marine invertebrates, sediment, and seawater around Alexandria, Egypt.

    abstract::Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-187

    authors: El-Sahn MA,El-Banna AA,El-Tabey Shehata AM

    更新日期:1982-11-01 00:00:00

  • Growth of group B streptococci in human serum leads to increased cell surface sialic acid and decreased activation of the alternative complement pathway.

    abstract::Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-016

    authors: Platt MW,Correa N Jr,Mold C

    更新日期:1994-02-01 00:00:00

  • [Effect of sunlight penetration on the development of phototrophic sulfur bacteria in marine environment].

    abstract::Bottles of medium were inoculated with both strains of Chromatiacea and Chlorobiaceae (mixed cultures) and immersed at differnt depths in the marine coastal waters of the Marseilles basin (France). After development, the in vivo absorption characteristics of the cells show the effect of the daylight penetration on the...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Matheron R,Baulaigue R

    更新日期:1977-03-01 00:00:00

  • Baculovirus-like particles in the reproductive tracts of female parasitoid wasps.

    abstract::Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-148

    authors: Stoltz DB,Vinson SB,MacKinnon EA

    更新日期:1976-07-01 00:00:00

  • Evaluation of bactericidal assay in serotyping Neisseria meningitidis group A.

    abstract::Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-139

    authors: Quan AL,Sippel JE

    更新日期:1979-08-01 00:00:00

  • Evidence for independent binding domains within a group A streptococcal type IIo IgG-binding protein.

    abstract::The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-149

    authors: Tsivitse M,Boyle MD

    更新日期:1996-11-01 00:00:00

  • Copper stress and filamentous fungus Humicola lutea 103 - ultrastructural changes and activities of key metabolic enzymes.

    abstract::Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-112

    authors: Krumova ETs,Stoitsova SR,Paunova-Krasteva TS,Pashova SB,Angelova MB

    更新日期:2012-12-01 00:00:00

  • Monoclonal IgM antibody protection in mice against infection with an encapsulated strain of Staphylococcus epidermidis.

    abstract::Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-065

    authors: Ichiman Y,Usui Y,Suganuma M,Yoshida K

    更新日期:1991-05-01 00:00:00

  • Biological H2 and CO oxidation activities are sensitive to compositional change of soil microbial communities.

    abstract::Trace gas uptake by microorganisms controls the oxidative capacity of the troposphere, but little is known about how this important function is affected by changes in soil microbial diversity. This article bridges that knowledge gap by examining the response of the microbial community-level physiological profiles (CLP...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0412

    authors: Saavedra-Lavoie J,de la Porte A,Piché-Choquette S,Guertin C,Constant P

    更新日期:2020-04-01 00:00:00

  • Directed biosynthesis of novel derivatives of echinomycin. II. Purification and structure elucidation.

    abstract::New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-112

    authors: Gauvreau D,Waring MJ

    更新日期:1984-06-01 00:00:00