Abstract：
:Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose, ribose, glucose, galactose, and mannose. The wall material that was released contained only 47% amino acids and was enriched in lipid, glucose, and phosphate. These results support data from electron micrographs, showing the localized release of cell wall material by the disulfide bond-breaking reagent at alkaline pH. In amino acid composition the untreated walls did not differ greatly from the material released by dithiothreitol, but differed considerably from the walls of another strain of M. hungatii. The ratios of the amino acids found in the cell wall proteins of several archaebacteria and of Bacillus cereus spore coats were similar.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Sprott GD,McKellar RCdoi
10.1139/m80-017subject
Has Abstractpub_date
1980-02-01 00:00:00pages
115-20issue
2eissn
0008-4166issn
1480-3275journal_volume
26pub_type
杂志文章abstract：:Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-116
更新日期：2008-02-01 00:00:00
abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0755
更新日期：2014-03-01 00:00:00
abstract：:Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/w2012-025
更新日期：2012-05-01 00:00:00
abstract：:A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-222
更新日期：1979-12-01 00:00:00
abstract：:A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-004
更新日期：1981-01-01 00:00:00
abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-033
更新日期：1992-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-093
更新日期：1978-05-01 00:00:00
abstract：:Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-119
更新日期：2005-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-093
更新日期：2007-11-01 00:00:00
abstract：:Heat activation (70 degrees C for 20 min) resulted in alteration in structural proteins and enzymes found in Bacillus cereus spore coats. The three notable changes were increased glycosylation of coat proteins, alteration in polypeptide pattern on sodium dodecyl sulfate - polyacrylamide gels, and an increase in free S...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-062
更新日期：1981-04-01 00:00:00
abstract：:Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-164
更新日期：1984-08-01 00:00:00
abstract：:Legionella pneumophila grows in amoebae and has achieved the ability to grow at various temperatures, although the mechanisms controlling this ability remain poorly understood. The Icm/Dot type IVB secretion system is composed of more than 25 proteins and is known to be essential for intracellular growth. The role of...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-011
更新日期：2012-04-01 00:00:00
abstract：:Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w98-225
更新日期：1999-02-01 00:00:00
abstract：:Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-239
更新日期：1975-10-01 00:00:00
abstract：:The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-114
更新日期：2000-12-01 00:00:00
abstract：:The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-105
更新日期：2007-12-01 00:00:00
abstract：:The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0261
更新日期：2020-01-01 00:00:00
abstract：:The study of diaminopimelic acid (DAP) incorporation and turnover during growth recovery in chloramphenicol-treated (CMP-treated) Bacillus megaterium cells showed that two kinds of turnover occurred. A low acid-soluble turnover appeared as soon as growth resumed in bacteria labeled before the CMP treatment and at the ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-051
更新日期：1980-03-01 00:00:00
abstract：:In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-028
更新日期：1975-02-01 00:00:00
abstract：:Microbial mats that develop in shallow brackish and hyposaline ponds in the rims of two French polynesian atolls (Rangiroa and Tetiaroa) were intensively investigated during the past three years. Comparative assessment of these mats (called kopara in polynesian language) showed remarkable similarities in their composi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-279
更新日期：1975-12-01 00:00:00
abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-002
更新日期：2007-04-01 00:00:00
abstract：:Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-012
更新日期：2006-06-01 00:00:00
abstract：:A proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (NAD) dependent oxidation of proline and the NADH-dependent reduction of delta 1-pyrroline 5-carboxylic acid (PCA) was purified from extracts of Clostridium sporogenes. Following purification, only one protein band was found on analytical po...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-147
更新日期：1981-09-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-041
更新日期：2012-05-01 00:00:00
abstract：:The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-239
更新日期：1976-11-01 00:00:00
abstract：:Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-115
更新日期：2010-02-01 00:00:00
abstract：:Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-112
更新日期：2012-12-01 00:00:00
