Abstract:
:Culture-independent, biomarker-targeted bacterial enumeration and identification strategies were employed to estimate total bacterial burden and diversity within the cabin air of commercial airliners. Samples from each of 4 flights on 2 commercial carriers were collected via air-impingement. The total viable microbial population ranged from below detection limits to 4.1 x 10(6) cells/m(3) of air, as assessed by the ATP assay. A gradual accumulation of microbes was observed from the time of passenger boarding through mid-flight, followed by a sharp decline in bacterial abundance and viability from the initiation of descent through landing. Representatives of the alpha-, beta-, and gamma-Proteobacteria, as well as Gram-positive bacteria, were isolated in varying abundance. Neisseria meningitidis rRNA gene sequences were retrieved in great abundance from Airline A followed by Streptococcus oralis/mitis sequences. Pseudomonas synxantha sequences dominated Airline B clone libraries, followed by those of N. meningitidis and S. oralis/mitis. The cabin air samples examined herein housed low bacterial diversity and were often dominated by a particular subset of bacteria: opportunistic pathogenic inhabitants of the human respiratory tract and oral cavity.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
La Duc MT,Stuecker T,Venkateswaran Kdoi
10.1139/W07-093subject
Has Abstractpub_date
2007-11-01 00:00:00pages
1259-71issue
11eissn
0008-4166issn
1480-3275pii
w07-093journal_volume
53pub_type
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journal_title:Canadian journal of microbiology
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