A diaminopimelic acid auxotrophic Escherichia coli donor provides improved counterselection following intergeneric conjugation with actinomycetes.

abstract：:The gene coding for glycoprotein B2 (gB2) of herpes simplex virus type 2 (HSV-2) strain 333 was mapped and its nucleotide sequence determined. Open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gB1 of HSV type 1. Several predicted features of gB2 are consistent...

journal_title：Canadian journal of microbiology

authors： Zwaagstra JC,Leung WC

更新日期：1987-10-01 00:00:00

abstract：:Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...

journal_title：Canadian journal of microbiology

authors： Emyanitoff RG,Rucinsky TE,Birdsell DC

更新日期：1976-06-01 00:00:00

abstract：:The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...

journal_title：Canadian journal of microbiology

authors： Maki ML,Lawrence JR,Swerhone GD,Leung KT

更新日期：2009-10-01 00:00:00

abstract：:Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...

journal_title：Canadian journal of microbiology

authors： Tershak DR

更新日期：1985-12-01 00:00:00

abstract：:Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...

journal_title：Canadian journal of microbiology

authors： Amils R,Ramírez L,Sanz JL,Marín I,Pisabarro AG,Ureña D

更新日期：1989-01-01 00:00:00

abstract：:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...

journal_title：Canadian journal of microbiology

authors： Duarte M,Cottenceau G,Portrait V,Pons AM

更新日期：2001-09-01 00:00:00

abstract：:Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...

journal_title：Canadian journal of microbiology

authors： Macias EA,Rana F,Blazyk J,Modrzakowski MC

更新日期：1990-08-01 00:00:00

abstract：:A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...

journal_title：Canadian journal of microbiology

authors： Giraudo AT,Raspanti CG,Calzolari A,Nagel R

更新日期：1994-08-01 00:00:00

abstract：:Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...

journal_title：Canadian journal of microbiology

authors： Sprott GD,McKellar RC

更新日期：1980-02-01 00:00:00

abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

journal_title：Canadian journal of microbiology

authors： Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

更新日期：2006-11-01 00:00:00

abstract：:Three strains, Rhodotorula rubra, Rhodotorula glutinis, and Candida zeylanoides, isolated from fish, were tested for the expression of putative tissue colonization factors. All strains were able to bind collagen type I, fibronectin, and laminin to various degrees after growing on various solid and broth media, while t...

journal_title：Canadian journal of microbiology

authors： Vázquez-Juárez R,Ascencio F,Andlid T,Gustafsson L,Wadström T

更新日期：1993-12-01 00:00:00

abstract：:Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...

journal_title：Canadian journal of microbiology

authors： Kishimoto RA,Eveland WC

更新日期：1975-12-01 00:00:00

abstract：:A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...

journal_title：Canadian journal of microbiology

authors： King EJ,Brown MF

更新日期：1983-06-01 00:00:00

abstract：:The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...

journal_title：Canadian journal of microbiology

authors： Gurwith MJ,Langston C,Dunsmore B

更新日期：1982-01-01 00:00:00

abstract：:Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...

journal_title：Canadian journal of microbiology

authors： April TM,Foght JM,Currah RS

更新日期：2000-01-01 00:00:00

abstract：:Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...

journal_title：Canadian journal of microbiology

authors： Pileggi SA,Vieira de Oliveira SF,Andrade CW,Vicente VA,Dalzoto Pdo R,Kniphoff da Cruz G,Gabardo J,Massola NS,Tozze HJ,Pileggi M,Kava-Cordeiro V,Galli-Terasawa LV,Pimentel IC,Stringari D,Glienke C

更新日期：2009-09-01 00:00:00

abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...

journal_title：Canadian journal of microbiology

authors： Van Horn KG,Smibert RM

更新日期：1982-03-01 00:00:00

abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...

journal_title：Canadian journal of microbiology

authors： Abelovska L,Bujdos M,Kubova J,Petrezselyova S,Nosek J,Tomaska L

更新日期：2007-04-01 00:00:00

abstract：:A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...

journal_title：Canadian journal of microbiology

authors： Farinha MA,Kropinski AM

更新日期：1997-03-01 00:00:00

abstract：:Growth rates and cell yields of Mycobacterium convolutum strain R22 grown on a wide range of both odd- and even-numbered carbon solid n-alkanes decreased as the substrate carbon number increased. Cellular lipid was 2.5 times higher following growth on the hydrocarbon substrates. The amount of polar lipid was found to ...

journal_title：Canadian journal of microbiology

authors： Hallas LE,Vestal JR

更新日期：1978-10-01 00:00:00

abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...

journal_title：Canadian journal of microbiology

authors： Stoltz DB,Vinson SB,MacKinnon EA

更新日期：1976-07-01 00:00:00

abstract：:A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...

journal_title：Canadian journal of microbiology

authors： Hawirko RZ,Naccarato CA,Lee RP,Maeba PY

更新日期：1979-04-01 00:00:00

abstract：:Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...

journal_title：Canadian journal of microbiology

authors： Niu YD,Stanford K,Ackermann HW,McAllister TA

更新日期：2012-07-01 00:00:00

abstract：:Six effective Rhizobium leguminosarum bv. phaseoli strains were examined for nodulation competitiveness on common bean (Phaseolus vulgaris L.), using all possible two-strain combinations of inoculum. Nodule occupancy was determined with strain-specific fluorescent antibodies. The strains were divided into three groups...

journal_title：Canadian journal of microbiology

authors： George ML,Robert FM

更新日期：1992-02-01 00:00:00

abstract：:Escherichia coli is capable of producing several virulence factors involved in the pathophysiology of bovine mastitis. Cell surface structures such as flagella, pili, and other outer membrane adhesins are considered essential for biofilm formation on abiotic surfaces. The aim of this study was characterize the molecul...

journal_title：Canadian journal of microbiology

authors： Silva VO,Espeschit IF,Moreira MA

更新日期：2013-05-01 00:00:00

abstract：:Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cell...

journal_title：Canadian journal of microbiology

authors： Gilewicz M,Zacek M,Bertrand JC,Azoulay E

更新日期：1979-02-01 00:00:00

abstract：:Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...

journal_title：Canadian journal of microbiology

authors： Brown EG

更新日期：1987-09-01 00:00:00

abstract：:This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...

journal_title：Canadian journal of microbiology

authors： McFarlane BL,Embree JE,Embil JA,Artsob H,Weste JB,Rozee KR

更新日期：1981-11-01 00:00:00

abstract：:Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...

journal_title：Canadian journal of microbiology

authors： Wheeler MH,Tolmsoff WJ,Bell AA,Mollenhauer HH

更新日期：1978-03-01 00:00:00

abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...

journal_title：Canadian journal of microbiology

authors： Lapierre J,Marsolais G,Pilon P,Descôteaux JP

更新日期：1980-10-01 00:00:00