Abstract:
:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal contents was evaluated to detect the sequence of mcjA, the gene encoding the MccJ25 precursor. The two molecular techniques were compared with the commonly used cross-immunity tests. They generate accurate data with no obvious cross-reactions with other microcins. The results display that the producers of MccJ25 were widely distributed in the poultry intestinal habitat. The applications of these molecular methods will be useful in future studies of microcinogenic populations, and thus contribute to understand the relationships within the complex intestinal microbial ecosystem.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Duarte M,Cottenceau G,Portrait V,Pons AMsubject
Has Abstractpub_date
2001-09-01 00:00:00pages
877-82issue
9eissn
0008-4166issn
1480-3275journal_volume
47pub_type
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journal_title:Canadian journal of microbiology
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