Haemophilus paragallinarum secretes metalloproteases.

Abstract:

:Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine gelatin (0.1%). They were active in a broad pH range (4-9); pH 7.5 being the optimum. Protease activity was inhibited by 20 mmol EDTA/L and reactivated by calcium. The proteolytic activity was heat-stable at 40, 50, and 60 degrees C, but its activity diminished at 70 degrees C or higher. Secreted proteins partially degraded chicken immunoglobulin G (IgG) and cross-reacted with a polyclonal serum against a high molecular mass protease secreted by Actinobacillus pleuropneumoniae. Extracellular proteases could play a role in infectious coryza caused by H. paragallinarum.

journal_name

Can J Microbiol

authors

Rivero-García PC,Cruz CV,Alonso PS,Vaca S,Negrete-Abascal E

doi

10.1139/w05-067

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

893-6

issue

10

eissn

0008-4166

issn

1480-3275

pii

w05-067

journal_volume

51

pub_type

杂志文章
  • Rapid identification of Listeria species and screening for variants by melting curve and high-resolution melting curve analyses of the intergenic spacer region of the rRNA gene.

    abstract::The presence of any Listeria species in food may be an indicator of poor hygiene in food processing facilities. The biochemical identification of Listeria species is laborious and time consuming. Therefore, the development of novel identification methods that are rapid and simple to perform would be an asset. In this ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-054

    authors: Wang J,Yamada S,Ohashi E

    更新日期:2010-08-01 00:00:00

  • Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer.

    abstract::Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-126

    authors: Bruver RM,Ball JS,Tustanoff ER

    更新日期:1975-06-01 00:00:00

  • Effects of Gypsophila saponins on bacterial growth kinetics and on selection of subterranean clover rhizosphere bacteria.

    abstract::Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-052

    authors: Fons F,Amellal N,Leyval C,Saint-Martin N,Henry M

    更新日期:2003-06-01 00:00:00

  • Uneven genotypic diversity of Escherichia coli in fecal sources limits the performance of a library-dependent method of microbial source tracking on the southwestern French Atlantic coast.

    abstract::To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2020-0244

    authors: Garabetian F,Vitte I,Sabourin A,Moussard H,Jouanillou A,Mornet L,Lesne M,Lyautey E

    更新日期:2020-12-01 00:00:00

  • Evidence for independent binding domains within a group A streptococcal type IIo IgG-binding protein.

    abstract::The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-149

    authors: Tsivitse M,Boyle MD

    更新日期:1996-11-01 00:00:00

  • Bindings of plasma proteins to streptococci of serological group L with special reference to their immunoglobulin G Fc-receptor activity.

    abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-001

    authors: Lämmler C,Schaufuss P,Frede C,Blobel H

    更新日期:1988-01-01 00:00:00

  • Activation of proinflammatory response in human intestinal epithelial cells following Vibrio cholerae infection through PI3K/Akt pathway.

    abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-093

    authors: Bandyopadhaya A,Bhowmick S,Chaudhuri K

    更新日期:2009-11-01 00:00:00

  • IAA-producing rhizobacteria from chickpea (Cicer arietinum L.) induce changes in root architecture and increase root biomass.

    abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0399

    authors: Fierro-Coronado RA,Quiroz-Figueroa FR,García-Pérez LM,Ramírez-Chávez E,Molina-Torres J,Maldonado-Mendoza IE

    更新日期:2014-10-01 00:00:00

  • Sulfur isotope fractionation by Salmonella heidelberg: inverse isotope effects during growth on high concentrations of Na2SO3.

    abstract::During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-217

    authors: McCready RG,Krouse HR

    更新日期:1979-12-01 00:00:00

  • Overexpression, purification, and analysis of the c1 repressor protein of Pseudomonas aeruginosa bacteriophage D3.

    abstract::A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-030

    authors: Farinha MA,Kropinski AM

    更新日期:1997-03-01 00:00:00

  • The effect of flavones and flavonols on colonization of tomato plants by arbuscular mycorrhizal fungi of the genera Gigaspora and Glomus.

    abstract::No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-036

    authors: Scervino JM,Ponce MA,Erra-Bassells R,Bompadre J,Vierheilig H,Ocampo JA,Godeas A

    更新日期:2007-06-01 00:00:00

  • Death rates of bacterial spores: nonlinear survivor curves.

    abstract::Nonlinear survivor curves were obtained when spores of Bacillus cereus were heated in physiological saline solution. Curvilinear survivor curves did not appear to be caused by experimental artifacts but by the heterogeneity of spore population with regard to heat resistance. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-217

    authors: Han YW

    更新日期:1975-10-01 00:00:00

  • Lipopolysaccharide changes and cytoplasmic polyphosphate granule accumulation in Pseudomonas aeruginosa during growth on hexadecane.

    abstract::Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-049

    authors: Miguez CB,Beveridge TJ,Ingram JM

    更新日期:1986-03-01 00:00:00

  • Dynamic metabolomic responses of Escherichia coli to nicotine stress.

    abstract::Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0206

    authors: Ding L,Chen J,Zou J,Zhang L,Ye Y

    更新日期:2014-08-01 00:00:00

  • Oxygen toxicity in Treponema pallidum: deoxyribonucleic acid single-stranded breakage induced by low doses of hydrogen peroxide.

    abstract::The effect of hydrogen peroxide on Treponema pallidum was investigated. The in vitro loss of virulence (as measured by rabbit inoculation) of T. pallidum was accelerated by as low as 100 microM hydrogen peroxide in the complex maintenance medium used. Higher doses led to rapidly accelerated death with 500 microM hydro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-234

    authors: Steiner BM,Wong GH,Sutrave P,Graves S

    更新日期:1984-12-01 00:00:00

  • Investigations of fungal diversity in wooden structures and soils at historic sites on the Antarctic Peninsula.

    abstract::Investigations of microbial diversity in Antarctic are important to begin to understand ecosystem functioning and decomposition processes. This study documents fungi at 9 historic sites on the Antarctic Peninsula collected from wooden structures, other organic materials, and soils during a joint National Science Found...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W08-120

    authors: Arenz BE,Blanchette RA

    更新日期:2009-01-01 00:00:00

  • Inhibition of Fusarium moniliforme var. subglutinans, the causal agent of pine pitch canker, by the soil bacterium Arthrobacter sp.

    abstract::A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-004

    authors: Barrows-Broaddus J,Kerr TJ

    更新日期:1981-01-01 00:00:00

  • Immune responses of mice to inactivated rabies vaccine administered orally: potentiation by Quillaja saponin.

    abstract::Administered orally, Quillaja saponin markedly potentiated the humoral immune responses of mice fed inactivated rabies vaccine, and significantly increased their resistance to subsequent intracerebral challenge with live rabies virus. Although mean serum neutralizing antibody titres were generally 8- to 16-fold higher...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-078

    authors: Maharaj I,Froh KJ,Campbell JB

    更新日期:1986-05-01 00:00:00

  • Vacuolar localization of phosphorus in hyphae of Phialocephala fortinii, a dark septate fungal root endophyte.

    abstract::Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features o...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-018

    authors: Saito K,Kuga-Uetake Y,Saito M,Peterson RL

    更新日期:2006-07-01 00:00:00

  • Mapping of a new pan mutation in Escherichia coli K-12.

    abstract::A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-190

    authors: Manch JN

    更新日期:1981-11-01 00:00:00

  • Inhibition of L-lysine uptake by alpha-methyl lysine in Escherichia coli and Bacillus sphaericus.

    abstract::alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-095

    authors: Regenstreif CA,Kelland JG,Pansare SV,Vederas JC,Pickard MA

    更新日期:1986-06-01 00:00:00

  • The occurrence of Candida Albicans in Lake Ontario bathing beaches.

    abstract::There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-159

    authors: Sherry JP,Kuchma SR,Dutka BJ

    更新日期:1979-09-01 00:00:00

  • Bactéries sporulées et biofilms : un problème récurrent dans les lignes de production de lait reconstitué ou recombiné pasteurisé.

    abstract::In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is in...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.1139/cjm-2018-0435

    authors: Malek F

    更新日期:2019-06-01 00:00:00

  • The effect of farnesol on amino acid incorporation by a wild-type and cell-wall variant strain of Candida albicans.

    abstract::Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-050

    authors: Braun PC

    更新日期:2005-08-01 00:00:00

  • Occurrence and identification of microorganisms in compacted clay-based buffer material designed for use in a nuclear fuel waste disposal vault.

    abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-162

    authors: Stroes-Gascoyne S,Pedersen K,Haveman SA,Dekeyser K,Arlinger J,Daumas S,Ekendahl S,Hallbeck L,Hamon CJ,Jahromi N,Delaney TL

    更新日期:1997-12-01 00:00:00

  • Development and application of an indirect enzyme-linked immunosorbent assay using recombinant S1 for serological testing of porcine epidemic diarrhea virus.

    abstract::Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was express...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0240

    authors: Shan Y,Liu Y,Liu Z,Li G,Chen C,Luo H,Chen Y,Guo N,Shi X,Zhang X,Fang W,Li X

    更新日期:2019-05-01 00:00:00

  • Cyanide degradation by an Escherichia coli strain.

    abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-070

    authors: Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

    更新日期:1996-05-01 00:00:00

  • Cell-free, protein-synthesizing system of photosynthetic and heterotrophic Rhodospirillum rubrum.

    abstract::An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-042

    authors: Chow CT

    更新日期:1976-02-01 00:00:00

  • Helicobacter pylori produces unique filaments upon host contact in vitro.

    abstract::Helicobacter pylori exists in 2 distinct morphological states, helicoid and coccoid. Both have been observed in in vitro culture and in gastric biopsies. We visualized H. pylori during AGS cell infections using immunofluorescence microscopy. Anti-H. pylori mouse serum as well as human serum from H. pylori-positive pat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-042

    authors: Couturier MR,Stein M

    更新日期:2008-07-01 00:00:00

  • Soil microbial populations, community composition, and activity as affected by repeated applications of hog and cattle manure in eastern Saskatchewan.

    abstract::A field site near Humboldt, Saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. During each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-069

    authors: De Freitas JR,Schoenau JJ,Boyetchko SM,Cyrenne SA

    更新日期:2003-09-01 00:00:00