Abstract：
:Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features of hyphae, including the nature of the vacuolar system, and whether polyphosphate was present in vacuoles. Both living hyphae and hyphae that had been rapidly frozen and freeze substituted before embedding were studied. A complex system of vacuoles, including a motile tubular vacuolar system, elongated vacuoles, and spherical vacuoles, was demonstrated in living hyphae by the fluorescent probe Oregon Green 488 carboxylic acid diacetate, using laser scanning confocal microscopy. The motile tubular vacuolar system was more prevalent at the hyphal tip than in more distal regions, whereas elongated vacuoles and spherical vacuoles were more abundant distal to the tip. All vacuoles contained polyphosphate as shown by labelling embedded samples with recombinant polyphosphate binding domain of Escherichia coli exopolyphosphatase, containing Xpress tag at the N-terminal end, followed by anti-Xpress antibody and a secondary antibody conjugated either to a fluorescent probe for laser scanning confocal microscopy or colloidal gold for transmission electron microscopy. The polyphosphate was dispersed in vacuoles. This was confirmed by staining embedded samples with 4',6-diamidino-2-phenylindole and viewing with UV light using epifluorescence microscopy. These cytological methods showed that the tubular vacuolar system had lower concentrations of polyphosphate than the spherical vacuoles. Lipid bodies were present around vacuoles.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Saito K,Kuga-Uetake Y,Saito M,Peterson RLdoi
10.1139/w06-018subject
Has Abstractpub_date
2006-07-01 00:00:00pages
643-50issue
7eissn
0008-4166issn
1480-3275pii
w06-018journal_volume
52pub_type
杂志文章abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-155
更新日期：1996-12-01 00:00:00
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更新日期：2000-11-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：2011-08-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：2000-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：2011-12-01 00:00:00
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doi：10.1139/m90-010
更新日期：1990-01-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：1976-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0383
更新日期：2014-11-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：1998-01-01 00:00:00
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更新日期：1986-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0561
更新日期：2016-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-031
更新日期：1979-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
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更新日期：2020-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0874
更新日期：2014-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-158
更新日期：1977-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-018
更新日期：1976-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-143
更新日期：2002-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2001-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
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更新日期：2005-03-01 00:00:00
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更新日期：1976-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1139/m83-106
更新日期：1983-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：1975-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1979-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：1989-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：2003-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1982-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-177
更新日期：1983-09-01 00:00:00
