Rapid identification of Listeria species and screening for variants by melting curve and high-resolution melting curve analyses of the intergenic spacer region of the rRNA gene.

abstract：:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...

journal_title：Canadian journal of microbiology

authors： Langley S,Beveridge TJ

更新日期：1999-07-01 00:00:00

abstract：:Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...

journal_title：Canadian journal of microbiology

authors： Konowalchuk J,Spiers JI

更新日期：1977-09-01 00:00:00

abstract：:Lyme disease is a tick-borne disease that is emerging in Canada. The disease is caused by spirochetes of the Lyme borreliosis group, which is expanding as new species are discovered. In Canada, Lyme disease risk has so far been assessed primarily by detection of Borrelia burgdorferi sensu stricto. Of Ixodes scapularis...

journal_title：Canadian journal of microbiology

authors： Lewis J,Lloyd VK

更新日期：2019-02-01 00:00:00

abstract：:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...

journal_title：Canadian journal of microbiology

authors： Davidson EW,Shellabarger C,Meyer M,Bieber AL

更新日期：1987-11-01 00:00:00

abstract：:A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...

journal_title：Canadian journal of microbiology

authors： Onishi H,Hidaka O

更新日期：1978-09-01 00:00:00

abstract：:Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged t...

journal_title：Canadian journal of microbiology

authors： Hochstein LI,Dalton BP,Pollock G

更新日期：1976-08-01 00:00:00

abstract：:The ability to rapidly identify and quantify a microbial strain in a complex environmental sample has widespread applications in ecology, epidemiology, and industry. In this study, we describe a rapid method to obtain functionally specific genetic markers that can be used in conjunction with standard or real-time poly...

journal_title：Canadian journal of microbiology

authors： Hynes SS,Chaudhry O,Providenti MA,Smith ML

更新日期：2006-05-01 00:00:00

abstract：:This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...

journal_title：Canadian journal of microbiology

authors： McFarlane BL,Embree JE,Embil JA,Artsob H,Weste JB,Rozee KR

更新日期：1981-11-01 00:00:00

abstract：:From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...

journal_title：Canadian journal of microbiology

authors： Sahin F,Karasartova D,Özsan TM,Kiyan M,Karahan CZ,Tekeli A

更新日期：2013-04-01 00:00:00

abstract：:Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...

journal_title：Canadian journal of microbiology

authors： Wang W,An B,Feng L,He C,Luo H

更新日期：2018-11-01 00:00:00

abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

journal_title：Canadian journal of microbiology

authors： Golubev WI,Sampaio JP,Alves L,Golubev NW

更新日期：2004-12-01 00:00:00

abstract：:We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus Trichoderma, and other filamentous fungi (Botrytis cinerea, Aspergillus niger, Fusarium culmorum, and Penicillium purpurogenum). We found that five Trichoderma strains secreted HMMPs into the media after induction with b...

journal_title：Canadian journal of microbiology

authors： Mat'at'a M,Galádová H,Varečka L,Šimkovič M

更新日期：2019-09-01 00:00:00

abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...

journal_title：Canadian journal of microbiology

authors： Pereira EM,Silva-Queiroz SR,Cabrera Gomez JG,Silva LF

更新日期：2009-06-01 00:00:00

abstract：:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...

journal_title：Canadian journal of microbiology

authors： Ncango DM,Swart CW,Goldblatt ME,Pohl CH,Van Wyk PW,Botes PJ,Kock JL

更新日期：2008-06-01 00:00:00

abstract：:Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...

journal_title：Canadian journal of microbiology

authors： Allard N,Garneau D,Poulin-Laprade D,Burrus V,Brzezinski R,Roy S

更新日期：2015-08-01 00:00:00

abstract：:Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...

journal_title：Canadian journal of microbiology

authors： Kishimoto RA,Eveland WC

更新日期：1975-12-01 00:00:00

abstract：:It is important to understand the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils for evaluating the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, t...

journal_title：Canadian journal of microbiology

authors： Kang Y,Hao Y,Xia D,Shen M,Li Q,Hu J

更新日期：2017-07-01 00:00:00

abstract：:Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...

journal_title：Canadian journal of microbiology

authors： Ichiman Y,Usui Y,Suganuma M,Yoshida K

更新日期：1991-05-01 00:00:00

abstract：:An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...

journal_title：Canadian journal of microbiology

authors： Laishley EJ

更新日期：1975-11-01 00:00:00

abstract：:An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...

journal_title：Canadian journal of microbiology

authors： Kam N,Park YJ,Lee EY,Kim HS

更新日期：2011-12-01 00:00:00

abstract：:The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...

journal_title：Canadian journal of microbiology

authors： Miller P,Liu X,McMullen LM

更新日期：2015-02-01 00:00:00

abstract：:Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, derived from in vitro and in vivo cultures (wheat roots) of Pseudomona...

journal_title：Canadian journal of microbiology

authors： Barnett SJ,Alami Y,Singleton I,Ryder MH

更新日期：1999-04-01 00:00:00

abstract：:In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...

journal_title：Canadian journal of microbiology

authors： Roychowdhury HS,Kapoor M

更新日期：1988-02-01 00:00:00

abstract：:The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...

journal_title：Canadian journal of microbiology

authors： Bannatyne RM,Cheung R

更新日期：1980-02-01 00:00:00

abstract：:In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...

journal_title：Canadian journal of microbiology

authors： Ishiguro EE

更新日期：1979-10-01 00:00:00

abstract：:Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was express...

journal_title：Canadian journal of microbiology

authors： Shan Y,Liu Y,Liu Z,Li G,Chen C,Luo H,Chen Y,Guo N,Shi X,Zhang X,Fang W,Li X

更新日期：2019-05-01 00:00:00

abstract：:Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...

journal_title：Canadian journal of microbiology

authors： Cobos-Marín L,Montes-Vargas J,Zumarraga M,Cataldi A,Romano MI,Estrada-Garcia I,Gonzalez-y-Merchand JA

更新日期：2005-11-01 00:00:00

abstract：:Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on ag...

journal_title：Canadian journal of microbiology

authors： Kamp AM,Bidochka MJ

更新日期：2002-09-01 00:00:00

abstract：:Three distinct bacterial populations have been defined in the bovine rumen: the rumen fluid population; the population associated with food particles; and the population adherent to the rumen epithelium. Alkaline phosphatase activity has been reported in cells of the first two populations and here we report that assay...

journal_title：Canadian journal of microbiology

authors： Fay JP,Cheng KJ,Costerton JW

更新日期：1979-08-01 00:00:00

abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...

journal_title：Canadian journal of microbiology

authors： MacNeil L,Kauri T,Robertson W

更新日期：1995-08-01 00:00:00