Abstract:
:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which bound toxin efficiently. The toxin was bound in the cold to sensitive cells and these cells could be rescued from cytotoxicity for ca. 15 min after warming, by which time toxin appeared to be internalized. Binding was saturable. This toxin is apparently internalized by receptor-mediated endocytosis, probably involving a glycoprotein receptor containing N-acetyl-D-glucosamine. Evidence for toxin binding to lipids was not found. Antibody appeared to detect internalized toxin, and high concentrations of sugars inhibited cytotoxicity; these results along with evidence from a recent ultrastructural study suggest that this toxin may form pores in the cell membrane.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Davidson EW,Shellabarger C,Meyer M,Bieber ALdoi
10.1139/m87-173subject
Has Abstractpub_date
1987-11-01 00:00:00pages
982-9issue
11eissn
0008-4166issn
1480-3275journal_volume
33pub_type
杂志文章abstract::There is an increasing movement against use of antibiotic growth promoters in animal feed. Prebiotic supplementation is a potential alternative to enhance the host's natural defense through modulation of gut microbiota. In the present study, the effect of mannan oligosaccharide (MOS) and virginiamycin (VIRG) on cecal ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0899
更新日期:2014-05-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/m93-093
更新日期:1993-07-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2011-06-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/m86-078
更新日期:1986-05-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/w02-062
更新日期:2002-08-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:
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journal_title:Canadian journal of microbiology
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更新日期:2012-04-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/w01-129
更新日期:2002-01-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-043
更新日期:1989-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-170
更新日期:1985-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-049
更新日期:1983-03-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-014
更新日期:1976-01-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-025
更新日期:2005-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0481
更新日期:2015-04-01 00:00:00
abstract::Pinus monticola antimicrobial peptide 1 (Pm-AMP1) was expressed and purified from bacterial cell lysate and its identity and purity confirmed by Western blot analysis using the Pm-AMP1 antibody. Application of Pm-AMP1 resulted in visible hyphal growth inhibition of Cronartium ribicola , Phellinus sulphurascens , O...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-046
更新日期:2011-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-101
更新日期:2007-01-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1979-03-01 00:00:00
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更新日期:1989-01-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2009-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-079
更新日期:1976-04-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2010-11-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2014-01-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-11-01 00:00:00