Binding of the Bacillus sphaericus mosquito larvicidal toxin to cultured insect cells.

Abstract:

:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which bound toxin efficiently. The toxin was bound in the cold to sensitive cells and these cells could be rescued from cytotoxicity for ca. 15 min after warming, by which time toxin appeared to be internalized. Binding was saturable. This toxin is apparently internalized by receptor-mediated endocytosis, probably involving a glycoprotein receptor containing N-acetyl-D-glucosamine. Evidence for toxin binding to lipids was not found. Antibody appeared to detect internalized toxin, and high concentrations of sugars inhibited cytotoxicity; these results along with evidence from a recent ultrastructural study suggest that this toxin may form pores in the cell membrane.

journal_name

Can J Microbiol

authors

Davidson EW,Shellabarger C,Meyer M,Bieber AL

doi

10.1139/m87-173

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

982-9

issue

11

eissn

0008-4166

issn

1480-3275

journal_volume

33

pub_type

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