Abstract:
:Three composts (Ball, dairy, and greenhouse) were tested for the ability to suppress the development of Fusarium root and stem rot (caused by Fusarium oxysporum f. sp. radicis-cucumerinum) on greenhouse cucumber. Dairy and greenhouse composts significantly reduced disease severity (P = 0.05), while Ball compost had no effect. Assessment of total culturable microbes in the composts showed a positive relationship between disease suppressive ability and total population levels of pseudomonads. In vitro antagonism assays between compost-isolated bacterial strains and the pathogen showed that strains of Pseudomonas aeruginosa exhibited the greatest antagonism. In growth room trials, strains of P. aeruginosa and nonantagonistic Pseudomonas maculicola, plus 2 biocontrol strains of Pseudomonas fluorescens, were tested for their ability to reduce (i) survival of F. oxysporum, (ii) colonization of plants by the pathogen, and (iii) disease severity. Cucumber seedlings grown in compost receiving P. aeruginosa and P. fluorescens had reduced disease severity index scores after 8 weeks compared with control plants without bacteria. Internal stem colonization by F. oxysporum was significantly reduced by P. aeruginosa. The bacteria colonized plant roots at 1.9 × 10(6) ± 0.73 × 10(6) CFU·(g root tissue)-1 and survival was >107 CFU·(g compost)-1 after 6 weeks. The locus for 2,4-diacetylphloroglucinol production was detected by Southern blot analysis and confirmed by PCR. The production of the antibiotic 2,4-diacetylphloroglucinol in liquid culture by P. aeruginosa was confirmed by thin layer chromatography. These results demonstrate that composts containing antibiotic-producing P. aeruginosa have the potential to suppress diseases caused by Fusarium species.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Bradley GG,Punja ZKdoi
10.1139/w10-076subject
Has Abstractpub_date
2010-11-01 00:00:00pages
896-905issue
11eissn
0008-4166issn
1480-3275pii
w10-076journal_volume
56pub_type
杂志文章abstract::The fine structure of conidia and immature resting spores of the entomogenous fungus Entomophthora apiculata is described. The two cell types contain comparable organelles and quantities of lipid, and possess a similar cell wall structure. The large quantity of stored lipid and numerous mitochondria in each cell type ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-067
更新日期:1977-04-01 00:00:00
abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-070
更新日期:1996-05-01 00:00:00
abstract::The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...
journal_title:Canadian journal of microbiology
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更新日期:2008-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-131
更新日期:1996-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-131
更新日期:1978-07-01 00:00:00
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更新日期:2017-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-186
更新日期:1988-09-01 00:00:00
abstract::Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-049
更新日期:1983-03-01 00:00:00
abstract::Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-031
更新日期:1996-02-01 00:00:00
abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-014
更新日期:1976-01-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-018
更新日期:1977-02-01 00:00:00
abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::A bacterium C1010, isolated from the rhizospheres of cucumbers in fields in Korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (HHSL), and octadecanoyl homoserine lactone (OHSL). Morphological characteristics and 16S rRNA sequence analysis identified C1010 as Acinetobacter sp. strain C...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-083
更新日期:2004-11-01 00:00:00
abstract::Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular pho...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-212
更新日期:1983-10-01 00:00:00
abstract::Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1-5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [alpha]D +2...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-167
更新日期:1986-12-01 00:00:00
abstract::Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-171
更新日期:1986-12-01 00:00:00
abstract::Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-220
更新日期:1985-12-01 00:00:00
abstract::Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-209
更新日期:1983-10-01 00:00:00
abstract::A stable enrichment culture converting acetic acid to methane was successfully obtained from a pear waste digester, using a synthetic substrate solution with acetic acid as the main carbon source. This enrichment culture converted up to 10 mmol of acetic acid per litre per day at 35 degrees C and did not use hydrogen ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-194
更新日期:1976-09-01 00:00:00
abstract::The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...
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pub_type: 杂志文章
doi:10.1139/m94-089
更新日期:1994-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-139
更新日期:1987-09-01 00:00:00
abstract::Evidence has shown that individuals exposed to indoor toxic molds for extended periods of time have elevated risk of developing numerous respiratory illnesses. It is not clear at the cellular level what impact mold exposure has on the immune system. Herein, we show that 2 fungal volatiles (E)-2-octenal and oct-1-en-3-...
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pub_type: 杂志文章
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abstract::The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-036
更新日期:1976-02-01 00:00:00
abstract::The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-119
更新日期:1990-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-177
更新日期:1978-09-01 00:00:00
abstract::The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1984-01-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-221
更新日期:1979-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-178
更新日期:1980-09-01 00:00:00
abstract::Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1979-05-01 00:00:00