Abstract:
:Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by nucleotide-induced phosphorylation. Nucleotides antagonized the binding of diphtheria toxin to its cell surface receptor in a manner that correlated with the degree of protection. It was concluded that cellular protection from diphtheria toxin by nucleotides results from inhibition of toxin-receptor binding and that nucleotides therefore may serve as valuable research tools for future studies.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Middlebrook JL,Dorland RBdoi
10.1139/m79-046subject
Has Abstractpub_date
1979-03-01 00:00:00pages
285-90issue
3eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract::Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-220
更新日期:1985-12-01 00:00:00
abstract::This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0465
更新日期:2013-01-01 00:00:00
abstract::Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-085
更新日期:1985-05-01 00:00:00
abstract::Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-116
更新日期:2008-02-01 00:00:00
abstract::Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-123
更新日期:2009-03-01 00:00:00
abstract::From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0721
更新日期:2013-04-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-030
更新日期:1997-03-01 00:00:00
abstract::The effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of virulent bacteriophage PL-1 on a Lactobacillus casei S strain isolated from a lactic acid beverage fermentation was investigated. Catechin, caffeic, and gallic acids, commercially produced red, white, and champagne tann...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-162
更新日期:1985-09-01 00:00:00
abstract::A visual micromethod for measuring antifungal effects on germination and growth is described. The antifungal agent griseofulvin and the fungus Trichophyton mentagrophytes were used as materials to compare the micromethod with a standard assay based on dry mycelial weight. The micromethod was more sensitive than the we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-093
更新日期:1978-05-01 00:00:00
abstract::The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0630
更新日期:2015-02-01 00:00:00
abstract::When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-035
更新日期:2008-06-01 00:00:00
abstract::An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-251
更新日期:1975-11-01 00:00:00
abstract::Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-002
更新日期:2007-04-01 00:00:00
abstract::Six effective Rhizobium leguminosarum bv. phaseoli strains were examined for nodulation competitiveness on common bean (Phaseolus vulgaris L.), using all possible two-strain combinations of inoculum. Nodule occupancy was determined with strain-specific fluorescent antibodies. The strains were divided into three groups...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-026
更新日期:1992-02-01 00:00:00
abstract::Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-075
更新日期:1991-06-01 00:00:00
abstract::Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-245
更新日期:1976-11-01 00:00:00
abstract::Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, derived from in vitro and in vivo cultures (wheat roots) of Pseudomona...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-006
更新日期:1999-04-01 00:00:00
abstract::Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-029
更新日期:1979-02-01 00:00:00
abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-014
更新日期:1976-01-01 00:00:00
abstract::Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-083
更新日期:2005-11-01 00:00:00
abstract::Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-068
更新日期:2007-08-01 00:00:00
abstract::This paper reports two different enterotoxigenic classes of the genus Aeromonas isolated from fishes. Strains of A. sobria were isolated from healthy fishes only wheras strains of A. hydrophila were isolated from both healthy and moribund fishes. Aeromonas sobria and hydrophilia strains produced a cytotoxic factor and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-174
更新日期:1977-09-01 00:00:00
abstract::To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-096
更新日期:2005-12-01 00:00:00
abstract::Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0155
更新日期:2019-10-01 00:00:00
abstract::The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-100
更新日期:1989-06-01 00:00:00
abstract::Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-132
更新日期:1995-10-01 00:00:00
abstract::Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-218
更新日期:1977-10-01 00:00:00
abstract::Our freshwater caulobacter collection contains about 40 strains that are morphologically similar to Caulobacter crescentus. All elaborate a crystalline protein surface (S) layer made up of protein monomers 100-193 kDa in size. We conducted a comparative study of S-layer secretion in 6 strains representing 3 size group...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-046
更新日期:2004-09-01 00:00:00