Protection of mammalian cells from diphtheria toxin by exogenous nucleotides.

Abstract:

:Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by nucleotide-induced phosphorylation. Nucleotides antagonized the binding of diphtheria toxin to its cell surface receptor in a manner that correlated with the degree of protection. It was concluded that cellular protection from diphtheria toxin by nucleotides results from inhibition of toxin-receptor binding and that nucleotides therefore may serve as valuable research tools for future studies.

journal_name

Can J Microbiol

authors

Middlebrook JL,Dorland RB

doi

10.1139/m79-046

subject

Has Abstract

pub_date

1979-03-01 00:00:00

pages

285-90

issue

3

eissn

0008-4166

issn

1480-3275

journal_volume

25

pub_type

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