Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter.

abstract：:Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...

journal_title：Canadian journal of microbiology

authors： Higa AI,Massarini E,Cazzulo JJ

更新日期：1978-03-01 00:00:00

abstract：:The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...

journal_title：Canadian journal of microbiology

authors： Tsivitse M,Boyle MD

更新日期：1996-11-01 00:00:00

abstract：:Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...

journal_title：Canadian journal of microbiology

authors： Barberio C,Bianchi L,Pinzauti F,Lodi T,Ferrero I,Polsinelli M,Casalone E

更新日期：2007-02-01 00:00:00

abstract：:The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...

journal_title：Canadian journal of microbiology

authors： Nour SM,Cleyet-Marel JC,Beck D,Effosse A,Fernandez MP

更新日期：1994-05-01 00:00:00

abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...

journal_title：Canadian journal of microbiology

authors： Stroes-Gascoyne S,Pedersen K,Haveman SA,Dekeyser K,Arlinger J,Daumas S,Ekendahl S,Hallbeck L,Hamon CJ,Jahromi N,Delaney TL

更新日期：1997-12-01 00:00:00

abstract：:The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude ex...

journal_title：Canadian journal of microbiology

authors： Vaz AB,Mota RC,Bomfim MR,Vieira ML,Zani CL,Rosa CA,Rosa LH

更新日期：2009-12-01 00:00:00

abstract：:The shikimate metabolites are key precursors to a large number of natural products, including aromatic amino acids. Chorismic acid is an important branch point in the biosynthetic pathway to aromatic amino acids. Chorismic acid is also unique among natural products since it is the only compound known to undergo an enz...

journal_title：Canadian journal of microbiology

authors： Zamir LO,Devor KA,Jensen RA,Tiberio R,Sauriol F,Mamer O

更新日期：1991-04-01 00:00:00

abstract：:When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...

journal_title：Canadian journal of microbiology

authors： Trentini WC,Murray RG

更新日期：1975-02-01 00:00:00

abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...

journal_title：Canadian journal of microbiology

authors： Wicklow DT,Shotwell OL

更新日期：1983-01-01 00:00:00

abstract：:Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...

journal_title：Canadian journal of microbiology

authors： Larouche É,Généreux M,Tremblay MÈ,Rhouma M,Gasser MO,Quessy S,Côté C

更新日期：2020-10-01 00:00:00

abstract：:A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...

journal_title：Canadian journal of microbiology

authors： Giraudo AT,Raspanti CG,Calzolari A,Nagel R

更新日期：1994-08-01 00:00:00

abstract：:The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...

journal_title：Canadian journal of microbiology

authors： Zhang XB,Ohta Y

更新日期：1992-07-01 00:00:00

abstract：:Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...

journal_title：Canadian journal of microbiology

authors： Liao CH,Heberlein GT

更新日期：1979-02-01 00:00:00

abstract：:Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

journal_title：Canadian journal of microbiology

authors： Wang W,Sun M,Liu W,Zhang B

更新日期：2008-10-01 00:00:00

abstract：:Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...

journal_title：Canadian journal of microbiology

authors： Samuelson DA,Bezerra J

更新日期：1977-10-01 00:00:00

abstract：:The effects of seven thiophosphorus pesticides and their degradation products on estuarine ammonium oxidizers were examined. Using an axenic, closed-culture, high cell density assay, the pesticides caused little inhibition of ammonium oxidation. However, the degradation product of methyl parathion, p-aminophenol, sign...

journal_title：Canadian journal of microbiology

authors： Jones RD,Hood MA

更新日期：1980-11-01 00:00:00

abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...

journal_title：Canadian journal of microbiology

authors： Tamplin ML,Jalali R,Ahmed MK,Colwell RR

更新日期：1990-06-01 00:00:00

abstract：:A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...

journal_title：Canadian journal of microbiology

authors： Laszlo A,Eidus L

更新日期：1978-06-01 00:00:00

abstract：:Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...

journal_title：Canadian journal of microbiology

authors： Patriquin DG,Döbereiner J

更新日期：1978-06-01 00:00:00

abstract：:The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysi...

journal_title：Canadian journal of microbiology

authors： Kou X,Chen Q,Ju X,Liu H,Chen W,Xue Z

更新日期：2014-11-01 00:00:00

abstract：:Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...

journal_title：Canadian journal of microbiology

authors： MacInnes JI,Trust TJ,Crosa JH

更新日期：1979-05-01 00:00:00

abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...

journal_title：Canadian journal of microbiology

authors： Fox KF,Brown A

更新日期：1993-05-01 00:00:00

abstract：:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...

journal_title：Canadian journal of microbiology

authors： Langley S,Beveridge TJ

更新日期：1999-07-01 00:00:00

abstract：:Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1-5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [alpha]D +2...

journal_title：Canadian journal of microbiology

authors： Riou JY,Guibourdenche M,Perry MB,MacLean LL,Griffith DW

更新日期：1986-12-01 00:00:00

abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...

journal_title：Canadian journal of microbiology

authors： Boopathy R,Manning JF

更新日期：1996-12-01 00:00:00

abstract：:The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...

journal_title：Canadian journal of microbiology

authors： Semanchek JJ,Golden DA,Williams RC

更新日期：1999-03-01 00:00:00

abstract：:The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...

journal_title：Canadian journal of microbiology

authors： Martin I,Sawatzky P,Liu G,Allen V,Lefebvre B,Hoang L,Lovgren M,Haldane D,Caeseele PV,Horsman G,Garceau R,Ratnam S,Wong T,Gilmour M

更新日期：2013-10-01 00:00:00

abstract：:Numerous ultrastructural and biochemical analyses have been performed to characterize the cell wall composition and structure of Candida albicans. However, little investigation has focused on how subtle differences in cell wall structure influence the intracellular transport of amino acids and monosaccharides. In this...

journal_title：Canadian journal of microbiology

authors： Braun PC

更新日期：1999-05-01 00:00:00

abstract：:The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...

journal_title：Canadian journal of microbiology

authors： Oulé MK,Tano K,Bernier AM,Arul J

更新日期：2006-12-01 00:00:00

abstract：:The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...

journal_title：Canadian journal of microbiology

authors： Heitman TL,Frederick LM,Viste JR,Guselle NJ,Morgan UM,Thompson RC,Olson ME

更新日期：2002-06-01 00:00:00