Characterization of a Tn551-mutant of Staphylococcus aureus defective in the production of several exoproteins.

abstract：:The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rum...

journal_title：Canadian journal of microbiology

authors： Qian W,Li Z,Ao W,Zhao G,Wu J,Li G

更新日期：2017-05-01 00:00:00

abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...

journal_title：Canadian journal of microbiology

authors： Ogg JE,Lee SY,Ogg BJ

更新日期：1979-09-01 00:00:00

abstract：:A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min. ...

journal_title：Canadian journal of microbiology

authors： Manch JN

更新日期：1981-11-01 00:00:00

abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...

journal_title：Canadian journal of microbiology

authors： Regenstreif CA,Kelland JG,Pansare SV,Vederas JC,Pickard MA

更新日期：1986-06-01 00:00:00

abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...

journal_title：Canadian journal of microbiology

authors： Knoop FC

更新日期：1978-08-01 00:00:00

abstract：:Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cell...

journal_title：Canadian journal of microbiology

authors： Gilewicz M,Zacek M,Bertrand JC,Azoulay E

更新日期：1979-02-01 00:00:00

abstract：:Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...

journal_title：Canadian journal of microbiology

authors： Casida LE Jr,Liu KC

更新日期：1979-06-01 00:00:00

abstract：:The course of intranasal infection of Sendai virus in CBA and DBA mice was investigated in animals decomplemented with purified cobra venom factor. The mice were decomplemented either immediately before inoculation or at 4 days postinfection. Depletion of complement after the infection had been established had no appa...

journal_title：Canadian journal of microbiology

authors： Finnie JC,Aston WP,O'Shaughnessy MV,Stewart RB

更新日期：1982-05-01 00:00:00

abstract：:The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...

journal_title：Canadian journal of microbiology

authors： Diena BB,Ashton FE,Ryan A,Wallace R

更新日期：1978-02-01 00:00:00

abstract：:Two potassium (K)-bearing minerals, Nanjing feldspar and Suzhou illite, were used to investigate K mobilization by the wild-type strain NBT of Bacillus edaphicus, also labeled MPs+, selected for high activity in mobilizing potassium from minerals, and by four of its UV + LiCl mutants, MPs++, MPs+1, MPs+2, and MPs-. In...

journal_title：Canadian journal of microbiology

authors： Sheng XF,He LY

更新日期：2006-01-01 00:00:00

abstract：:Trace gas uptake by microorganisms controls the oxidative capacity of the troposphere, but little is known about how this important function is affected by changes in soil microbial diversity. This article bridges that knowledge gap by examining the response of the microbial community-level physiological profiles (CLP...

journal_title：Canadian journal of microbiology

authors： Saavedra-Lavoie J,de la Porte A,Piché-Choquette S,Guertin C,Constant P

更新日期：2020-04-01 00:00:00

abstract：:beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...

journal_title：Canadian journal of microbiology

authors： Venkateshwar M,Chaitanya K,Altaf MD,Hameeda B,Ghopal Reddy M

更新日期：2009-04-01 00:00:00

abstract：:A simplified procedure is presented for releasing and concentrating indigenous microbial cells from soil for viewing by transmission electron microscopy as thin sections or replicas of frozen-etched preparations. This procedure is compared with two others reported earlier, and their relative merits are discussed as co...

journal_title：Canadian journal of microbiology

authors： Balkwill DL,Labeda DP,Casida LE Jr

更新日期：1975-03-01 00:00:00

abstract：:An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...

journal_title：Canadian journal of microbiology

authors： Mehta RJ

更新日期：1977-04-01 00:00:00

abstract：:The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...

journal_title：Canadian journal of microbiology

authors： Tsivitse M,Boyle MD

更新日期：1996-11-01 00:00:00

abstract：:Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...

journal_title：Canadian journal of microbiology

authors： Mohanty SR,Kumar A,Parmar R,Dubey G,Patra AK,Kollah B

更新日期：2020-12-10 00:00:00

abstract：:The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...

journal_title：Canadian journal of microbiology

authors： Wisniewski JP,Delmotte FM

更新日期：1996-03-01 00:00:00

abstract：:The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...

journal_title：Canadian journal of microbiology

authors： Maki ML,Lawrence JR,Swerhone GD,Leung KT

更新日期：2009-10-01 00:00:00

abstract：:The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...

journal_title：Canadian journal of microbiology

authors： Kjosbakken J,Colvin JR

更新日期：1975-02-01 00:00:00

abstract：:Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...

journal_title：Canadian journal of microbiology

authors： Simard C,Mardini A,Bordeleau LM

更新日期：1975-06-01 00:00:00

abstract：:Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well...

journal_title：Canadian journal of microbiology

authors： Lambe DW Jr,Mayberry-Carson KJ,Ferguson KP

更新日期：1984-06-01 00:00:00

abstract：:During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...

journal_title：Canadian journal of microbiology

authors： Willison JH

更新日期：1985-07-01 00:00:00

abstract：:Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensifo...

journal_title：Canadian journal of microbiology

authors： Allen PZ,Connelly MC,Apicella MA

更新日期：1980-04-01 00:00:00

abstract：:In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...

journal_title：Canadian journal of microbiology

authors： Yoh M,Frimpong EK,Voravuthikunchai SP,Honda T

更新日期：1999-09-01 00:00:00

abstract：:Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...

journal_title：Canadian journal of microbiology

authors： Connolly JH,Chen Y,Jellison J

更新日期：1995-04-01 00:00:00

abstract：:A large pool of antibiotic resistant and auxotrophic mutants was isolated from the Staphylococcus aureus phage group 2 strains UT0002-19 and UT0017 by (1) antibiotic gradient plates, (2) trimethoprim selection, and (3) nitrosoguanidine mutagenesis, which sometimes was coupled by enrichment with either penicillin or me...

journal_title：Canadian journal of microbiology

authors： Martin SM,Rogolsky M

更新日期：1980-08-01 00:00:00

abstract：:A baseline serotype distribution was established by age and region for 2058 invasive Streptococcus pneumoniae isolates collected during the implementation period of the 13-valent pneumococcal conjugate vaccine (PCV13) program in many parts of Canada in 2010. Serotypes 19A, 7F, and 3 were the most prevalent in all ag...

journal_title：Canadian journal of microbiology

authors： Demczuk WH,Martin I,Griffith A,Lefebvre B,McGeer A,Shane A,Zhanel GG,Tyrrell GJ,Gilmour MW,Toronto Invasive Bacterial Diseases Network.,Canadian Public Health Laboratory Network.

更新日期：2012-08-01 00:00:00

abstract：:The cultural, morphological, and physiological properties of Thermomonospora fusca (strain 190Th) are described. Its physiological properties show that this species is primarily a carbohydrate-degrading actinomycete which can use a wide range of plant sugars and polymeric carbohydrates as sources of carbon and energy....

journal_title：Canadian journal of microbiology

authors： Crawford DL

更新日期：1975-11-01 00:00:00

abstract：:The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...

journal_title：Canadian journal of microbiology

authors： Lake JA

更新日期：1989-01-01 00:00:00

abstract：:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...

journal_title：Canadian journal of microbiology

authors： Ding L,Chen J,Zou J,Zhang L,Ye Y

更新日期：2014-08-01 00:00:00