Abstract：
:Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from Proteus TPases which differ themselves. The coli group TPases attach 4 mol of pyridoxal phosphate (PLP)/mol of enzyme, independently of the pH in the presence of K(plus) ions, and 9 mol of PLP/mol of enzyme must be reduced to achieve complete inactivation. The Proteus TPases attach 4 mol of PLP/mol of enzyme at PH 6.8, and 3 mol of PLP/mol of enzyme at pH 7.8 in K(plus) buffer. In P. morganii, 7 mol of PLP/mol of enzyme must be reduced to inactivate the enzyme, whereas P. vulgaris TPase cannot be completely inactivated by this method. These five TPases attach only 3 mol of PLP/mol of enzyme in a Na(plus) buffer, independently of the pH.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Simard C,Mardini A,Bordeleau LMsubject
Has Abstractpub_date
1975-06-01 00:00:00pages
834-40issue
6eissn
0008-4166issn
1480-3275journal_volume
21pub_type
杂志文章abstract：:An anaerobic consortium that carboxylated and dehydroxylated phenol to benzoate, and 2-cresol to 3-methylbenzoic acid, under methanogenic conditions was studied. Phenol induced this transformation activity. Addition of 4-hydroxypyridine or an increase in the concentration of proteose peptone to 0.5% (w/v) delayed the ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-093
更新日期：1993-07-01 00:00:00
abstract：:In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-184
更新日期：1983-09-01 00:00:00
abstract：:The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-036
更新日期：1976-02-01 00:00:00
abstract：:The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0239
更新日期：2014-07-01 00:00:00
abstract：:Vesicular-arbuscular mycorrhizae of yellow poplar (Liriodendron tulipifera L.) were examined by scanning electron microscopy. The morphology and spatial distribution of endophytic structures are illustrated. The use of stereoscopic techniques in conjunction with removal of host cytoplasm permits detection of subtle ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-146
更新日期：1975-07-01 00:00:00
abstract：:Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-046
更新日期：1979-03-01 00:00:00
abstract：:Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0254
更新日期：2020-12-10 00:00:00
abstract：:The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-077
更新日期：1989-04-01 00:00:00
abstract：:Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon, and iota) for classifica...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-015
更新日期：2002-04-01 00:00:00
abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-064
更新日期：1989-03-01 00:00:00
abstract：:The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0596
更新日期：2017-05-01 00:00:00
abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-099
更新日期：2004-12-01 00:00:00
abstract：:Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-020
更新日期：1988-02-01 00:00:00
abstract：:Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus tra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-111
更新日期：1996-08-01 00:00:00
abstract：:Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-195
更新日期：1984-10-01 00:00:00
abstract：:The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-009
更新日期：2008-04-01 00:00:00
abstract：:A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-055
更新日期：1999-08-01 00:00:00
abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-001
更新日期：1983-01-01 00:00:00
abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-186
更新日期：1988-09-01 00:00:00
abstract：:The susceptibility of congenitally immunodeficient mice to a nonencapsulated strain of Cryptococcus neoformans (strain M7) was evaluated. Gnotobiotic mice with defined congenital defects in innate immunity (beige) or cell-mediated immunity (athymic) or with combined defects in innate and cellular immunity (beige athym...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-144
更新日期：1991-11-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:A total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in Chesapeake Bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. In addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. Twenty-th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-212
更新日期：1977-10-01 00:00:00
abstract：:A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-027
更新日期：1993-02-01 00:00:00
abstract：:Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-115
更新日期：2008-01-01 00:00:00
abstract：:Enterococci, formerly confounded with faecal streptococci, are recognized since the beginning of the century as being faecal in origin and are generally searched for in waste waters and food products; their detection may in fact indicate the presence of enteropathogenic organisms. Although nearly ubiquitous, their pre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:The aim of the present study was to examine soil samples from various vegetation zones in terms of physicochemical properties, microbial communities, and isolation and identification (by polymerase chain reaction and transmission electron microscopy) of bacteria producing poly-β-hydroxybutyrates (PHBs). Soil samples w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-037
更新日期：2012-05-01 00:00:00
abstract：:The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-119
更新日期：1990-10-01 00:00:00
abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-112
更新日期：2004-02-01 00:00:00
abstract：:Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0464
更新日期：2019-06-01 00:00:00
