Abstract：
:The aim of the present study was to examine soil samples from various vegetation zones in terms of physicochemical properties, microbial communities, and isolation and identification (by polymerase chain reaction and transmission electron microscopy) of bacteria producing poly-β-hydroxybutyrates (PHBs). Soil samples were analysed originating from zones with heterogeneous environmental conditions from the Romanian Carpathian Mountains (mountain zone with alpine meadow, karstic zone with limestone meadow, hill zone with xerophilous meadow, and flood plain zone with hygrophilic meadow). Different bacterial groups involved in the nitrogen cycle (aerobic mesophilic heterotrophs, ammonifiers, denitrifiers, nitrifiers, and free nitrogen-fixing bacteria from Azotobacter genus) were analysed. Soil biological quality was assessed by the bacterial indicator of soil quality, which varied between 4.3 and 4.7. A colony polymerase chain reaction technique was used for screening PHB producers. With different primers, specific bands were obtained in all the soil samples. Some wild types of Azotobacter species were isolated from the 4 studied sites. Biodegradable polymers of PHB were assessed by negative staining in transmission electron microscopy. The maximum PHB granules density was obtained in the strains isolated from the xerophilous meadow (10-18 granules/cell), which was the most stressful environment from all the studied sites, as the physicochemical and microbiological tests proved.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Carpa R,Butiuc-Keul A,Lupan I,Barbu-Tudoran L,Muntean V,Dobrotă Cdoi
10.1139/w2012-037subject
Has Abstractpub_date
2012-05-01 00:00:00pages
660-7issue
5eissn
0008-4166issn
1480-3275journal_volume
58pub_type
杂志文章abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-162
更新日期：1997-12-01 00:00:00
abstract：:Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0612
更新日期：2013-06-01 00:00:00
abstract：:Nonmotile and motility-altered mutants of Myxococcus xanthus have been obtained by the use of chemical mutagens, ultraviolet irradiation, and a procedure for selective spontaneous mutants. As judged by their behaviour on a variety of growth media, in both plate and slide culture, the mutants were divided into four gro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-190
更新日期：1976-09-01 00:00:00
abstract：:During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-128
更新日期：1981-08-01 00:00:00
abstract：:Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w98-225
更新日期：1999-02-01 00:00:00
abstract：:Neisseria gonorrhoeae exist in transparent (Tr) and opaque (Op) colony forms. Op forms are recovered from patients early in the menstrual cycle; Tr colonies predominate late in the cycle. The mechanism for this colonial variation was examined by determining the influence of gonodal hormones on growth inhibition of Op ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-044
更新日期：1982-03-01 00:00:00
abstract：:Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-066
更新日期：2008-10-01 00:00:00
abstract：:Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-083
更新日期：1979-05-01 00:00:00
abstract：:Dense populations of anaerobic bacteria were found sequentially layered below the thermocline in two eutrophic lakes in southwest Michigan. Phase and electron microscopy of whole cells and thin sections were used to reveal the in situ morphology of the dominant members of the community. The predominant chlorophyll-con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-051
更新日期：1975-03-01 00:00:00
abstract：:Helicobacter pylori exists in 2 distinct morphological states, helicoid and coccoid. Both have been observed in in vitro culture and in gastric biopsies. We visualized H. pylori during AGS cell infections using immunofluorescence microscopy. Anti-H. pylori mouse serum as well as human serum from H. pylori-positive pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-042
更新日期：2008-07-01 00:00:00
abstract：:Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-132
更新日期：2007-02-01 00:00:00
abstract：:Enterococci, formerly confounded with faecal streptococci, are recognized since the beginning of the century as being faecal in origin and are generally searched for in waste waters and food products; their detection may in fact indicate the presence of enteropathogenic organisms. Although nearly ubiquitous, their pre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-073
更新日期：1997-06-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:The physiological characteristics and fatty acid composition of 40 strains of Yarrowia lipolytica isolated from irradiated poultry meat, commercial chilled foods, the superficial water of lagoons of an Italian river delta, and commercial light butter were compared. Discriminant analysis of the results permitted the 40...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-008
更新日期：1994-01-01 00:00:00
abstract：:The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-022
更新日期：1978-02-01 00:00:00
abstract：:Effects of temperature and anaerobic conditions on the replication of two bacteriophages, nt-1 and nt-6, of the estuarine bacterium Vibrio natriegens were studied. Reduction in temperature resulted in longer latent periods and reduced burst sizes for both phages. Replication under anaerobic conditions resulted in long...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-053
更新日期：1978-03-01 00:00:00
abstract：:Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus tra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-111
更新日期：1996-08-01 00:00:00
abstract：:In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water sampl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0618
更新日期：2013-03-01 00:00:00
abstract：:Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consorti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0323
更新日期：2017-01-01 00:00:00
abstract：:Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic C4 grass, that are commercially important forage grasses in tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-127
更新日期：2001-01-01 00:00:00
abstract：:The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-126
更新日期：1992-08-01 00:00:00
abstract：:The range of polysaccharide-degrading enzymes formed by three anaerobic rumen fungi (Neocallimastix patriciarum, Piromonas communis, and an unidentified isolate (F] was monitored following growth on seven mono-, di-, and poly-saccharide carbohydrate substrates. Enzymes capable of degrading a variety of alpha- and beta...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-071
更新日期：1987-05-01 00:00:00
abstract：:Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0528
更新日期：2015-12-01 00:00:00
abstract：:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W08-035
更新日期：2008-06-01 00:00:00
abstract：:In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-034
更新日期：2011-07-01 00:00:00
abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-201
更新日期：1980-10-01 00:00:00
abstract：:Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-058
更新日期：1995-04-01 00:00:00
abstract：:The use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. A 600-bp partial hsp60 gene was amplified by PCR and sequenced from 29 strains representing 15 Vibrio species within the family Vibrionaceae. Sequence comparison of the amplified partial hsp60 gene ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-089
更新日期：2002-10-01 00:00:00
