Abstract:
:The use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. A 600-bp partial hsp60 gene was amplified by PCR and sequenced from 29 strains representing 15 Vibrio species within the family Vibrionaceae. Sequence comparison of the amplified partial hsp60 gene revealed 71-82% sequence identity among different Vibrio species and 96-100% sequence identity among epidemiologically distinct strains with the same species designation. This degree of discrimination allows unambiguous differentiation of all Vibrio species included in the current study from each other, as well as from Aeromonas hydrophila and Plesiomonas shigelloides, which are often misidentified as Vibrio species by conventional biochemical methods. Based on the hsp60 gene sequences, two previously unidentified shrimp isolates were found to be more closely related to Vibrio alginolyticus (93-94% sequence identity) than to Vibrio parahaemolyticus (89% sequence identity), whereas 16S rRNA gene analysis was unable to differentiate among these closely related species (95-97% sequence identity). Our results indicate that the hsp60 gene may be a useful alternative target for phylogenetic analysis and species identification of marine Vibrios to complement more conventional identification systems.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Kwok AY,Wilson JT,Coulthart M,Ng LK,Mutharia L,Chow AWdoi
10.1139/w02-089subject
Has Abstractpub_date
2002-10-01 00:00:00pages
903-10issue
10eissn
0008-4166issn
1480-3275journal_volume
48pub_type
杂志文章abstract::Nitrogen-fixing, field-obtained root nodules of the silky green alder were studied by transmission electron microscopy. The nodule endophyte exhibited a prokaryotic cytology and was present in two forms: the hypha(0.3-1.0mum), which was branched and septate, and the vesicle (3-5mum), which was also septate and develo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-157
更新日期:1975-07-01 00:00:00
abstract::Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0612
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journal_title:Canadian journal of microbiology
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doi:
更新日期:2001-07-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
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abstract::Cultures of Bacillus subtilis lysogenic for the temperature bacteriophage SP beta release "betacin", a bacteriocinlike substance that inhibits B. subtilis strains which do not carry this phage. Production of betacin is blocked by mutations in the bet gene on the prophage and a second phage gene, tol, is apparently inv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-220
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abstract::The techniques in current use for detection of pathogens in environmental samples are restricted to those organisms whose replication in either culture media or cell culture is feasible. These methods lack the selectivity and sensitivity necessary for their unequivocal detection and identification. We have developed a...
journal_title:Canadian journal of microbiology
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doi:10.1139/m92-062
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abstract::The effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of virulent bacteriophage PL-1 on a Lactobacillus casei S strain isolated from a lactic acid beverage fermentation was investigated. Catechin, caffeic, and gallic acids, commercially produced red, white, and champagne tann...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-162
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-067
更新日期:1977-04-01 00:00:00
abstract::An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escheri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-020
更新日期:1993-01-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/w02-098
更新日期:2002-11-01 00:00:00
abstract::HPr is a protein of the phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In gram-positive bacteria, HPr can be phosphorylated on Ser-46 by the kinase/phosphorylase HprK/P and on His-15 by phospho-enzyme I (EI~P) of the PTS. In vitro studies with purified HPrs from Bacillus subtilis, Enterococcus fa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-085
更新日期:2008-11-01 00:00:00
abstract::The bacterial bioluminescence assay is one of the novel means for toxicity detection. The bioluminescence response of 2 marine bioluminescent bacteria was tested upon their long-term exposure to 9 different reverse osmosis (RO) rejects with varying chemical composition sampled from various dye industries. Bioluminesce...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0378
更新日期:2014-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-102
更新日期:2007-01-01 00:00:00
abstract::Several strains of Rhizobium japonicum have been reported to consist of mixtures of stable derivativess having distinct colony morphologies and physiological characteristics. We isolated derivatives from strains of R. japonicum and systematically compared them with previously isolated derivatives with respect to the u...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-106
更新日期:1980-05-01 00:00:00
abstract::Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-303
更新日期:1975-12-01 00:00:00
abstract::Morphological differentiation has commanded attention for its putative impact on the pathogenesis of invasive fungal infections. We evaluated in vitro and in vivo the dimorphism from mycelial to yeast-phase of Sporothrix schenckii, Blastomyces dermatitidis and Paracoccidioides brasiliensis isolates, two strains for ea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-026
更新日期:2004-06-01 00:00:00
abstract::A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-186
更新日期:1975-08-01 00:00:00
abstract::Mutants of Trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. These included strains whose activity in a Fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. Characteris...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-112
更新日期:1991-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1139/cjm-2014-0747
更新日期:2015-05-01 00:00:00
abstract::The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-039
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-124
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abstract::Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-064
更新日期:1976-03-01 00:00:00
abstract::Trace gas uptake by microorganisms controls the oxidative capacity of the troposphere, but little is known about how this important function is affected by changes in soil microbial diversity. This article bridges that knowledge gap by examining the response of the microbial community-level physiological profiles (CLP...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0412
更新日期:2020-04-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-106
更新日期:2007-12-01 00:00:00
abstract::Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-020
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abstract::Group B Neisseria meningitidis (SD1C) was grown on defined medium supplemented with each of a variety of sulphur compounds as the sole source of sulphur. The organism grew on sulphate, sulphite, bisulphite, thiosulphate, dithionite, hydrosulphide, thiocyanate, L-cysteine, L-cystine, reduced glutathione, methionine, me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-232
更新日期:1984-12-01 00:00:00
abstract::The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-130
更新日期:1976-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0493
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-174
更新日期:1976-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00